New immobilisation method for oligonucleotides on electrodes enables highly-sensitive, electrochemical label-free gene sensing

Aydemir, Nihan; Chan, Edward; Baek, Paul; Barker, David; Williams, David E.; Travaš-Sejdić, Jadranka

doi:10.1016/j.bios.2017.05.049

Cited by 26 publications

(21 citation statements)

References 64 publications

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“…Aydemir et al [93] reported an alternative immobilization method for capturing oligonucleotides. An amine-terminated captured DNA was covalently attached to carboxylic acid-functionalized pyrrole phenylene or thiophene phenylene monomers via carbodiimide chemistry.…”

Section: Conducting Polymer-based Electrochemical Sensorsmentioning

confidence: 99%

Biomedical Application of Electroactive Polymers in Electrochemical Sensors: A Review

2019

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Conducting polymers are of interest due to their unique behavior on exposure to electric fields, which has led to their use in flexible electronics, sensors, and biomaterials. The unique electroactive properties of conducting polymers allow them to be used to prepare biosensors that enable real time, point of care (POC) testing. Potential advantages of these devices include their low cost and low detection limit, ultimately resulting in increased access to treatment. This article presents a review of the characteristics of conducting polymer-based biosensors and the recent advances in their application in the recognition of disease biomarkers.

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Section: Conducting Polymer-based Electrochemical Sensorsmentioning

confidence: 99%

Biomedical Application of Electroactive Polymers in Electrochemical Sensors: A Review

2019

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“…Urease was covalently linked to the surface carboxyl group of the P(3HT-3TAA) film and the resultant urease-immobilized P(3HT-3TAA) film electrode had a detectable concentration range of about 1‒5 mM (the normal level of urea in blood serum is 1.3–3.5 mM). Travas-Sejdic used pyrrole and thiophene derivative monomers, such as carboxylic acid-bearing pyrrole and thiophene phenylenes, to immobilize receptors on the resulting polymer film [31]. Oligonucleotides were readily attached to the pyrrole-/thiophene-based termonomers using carbodiimide chemistry, and subsequent electrochemical polymerization resulted in conductive polymer thin films, which allowed selective DNA detection.…”

Section: Nanostructure: Nanoparticlesmentioning

confidence: 99%

Chemical Design of Functional Polymer Structures for Biosensors: From Nanoscale to Macroscale

et al. 2018

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Over the past decades, biosensors, a class of physicochemical detectors sensitive to biological analytes, have drawn increasing interest, particularly in light of growing concerns about human health. Functional polymeric materials have been widely researched for sensing applications because of their structural versatility and significant progress that has been made concerning their chemistry, as well as in the field of nanotechnology. Polymeric nanoparticles are conventionally used in sensing applications due to large surface area, which allows rapid and sensitive detection. On the macroscale, hydrogels are crucial materials for biosensing applications, being used in many wearable or implantable devices as a biocompatible platform. The performance of both hydrogels and nanoparticles, including sensitivity, response time, or reversibility, can be significantly altered and optimized by changing their chemical structures; this has encouraged us to overview and classify chemical design strategies. Here, we have organized this review into two main sections concerning the use of nanoparticles and hydrogels (as polymeric structures) for biosensors and described chemical approaches in relevant subcategories, which act as a guide for general synthetic strategies.

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“…Despite the rapid research growth in flexible biosensors and bioelectronics, there is still a need for better integration of electrically active materials with biological systems, including the better matching of the mechanical properties of these materials with the biological entities and the improvement of the robustness of the interfaces. Consequently, recent research has focused on creating CP materials and their composites with enhanced properties and functionality …”

Section: Introductionmentioning

confidence: 99%

Flexible and Stretchable PEDOT‐Embedded Hybrid Substrates for Bioengineering and Sensory Applications

et al. 2019

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Herein, we introduce a flexible, biocompatible, robust and conductive electrospun fiber mat as a substrate for flexible and stretchable electronic devices for various biomedical applications. To impart the electrospun fiber mats with electrical conductivity, poly(3,4‐ethylenedioxythiophene) (PEDOT), a conductive polymer, was interpenetrated into nitrile butadiene rubber (NBR) and poly(ethylene glycol)dimethacrylate (PEGDM) crosslinked electrospun fiber mats. The mats were fabricated with tunable fiber orientation, random and aligned, and displayed elastomeric mechanical properties and high conductivity. In addition, bending the mats caused a reversible change in their resistance. The cytotoxicity studies confirmed that the elastomeric and conductive electrospun fiber mats support cardiac cell growth, and thus are adaptable to a wide range of applications, including tissue engineering, implantable sensors and wearable bioelectronics.

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New immobilisation method for oligonucleotides on electrodes enables highly-sensitive, electrochemical label-free gene sensing

Cited by 26 publications

References 64 publications

Biomedical Application of Electroactive Polymers in Electrochemical Sensors: A Review

Biomedical Application of Electroactive Polymers in Electrochemical Sensors: A Review

Chemical Design of Functional Polymer Structures for Biosensors: From Nanoscale to Macroscale

Flexible and Stretchable PEDOT‐Embedded Hybrid Substrates for Bioengineering and Sensory Applications

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