New Insights in Bladder Cancer Diagnosis: Urinary miRNAs and Proteins

Amuran, Gökçe Gūllū; Eyüboğlu, İrem Peker; Tınay, İlker; Akkiprik, Mustafa

doi:10.3390/medsci6040113

Cited by 19 publications

(17 citation statements)

References 86 publications

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“…Other miRNAs with an altered expression level in bladder cancer involved in EMT and invasion is miR-141. In multiple other pathologies [30], not only bladder cancer [31], miR-200c and miR-141 were considered prognostic markers [32]. It should be noted that for the range of bladder cancer samples studied, the expression levels of miR-205 and miR-200c are highly disputed [22, 33, 34], often reported as both downregulated [35] and overexpressed [17].…”

Section: Discussionmentioning

confidence: 99%

Connecting the dots between different networks: miRNAs associated with bladder cancer risk and progression

Braicu

Buiga

Cojocneanu

et al. 2019

J Exp Clin Cancer Res

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BackgroundBladder cancer (BC) is a common urothelial malignancy, characterized by a high recurrence rate. The biology of bladder cancer is complex and needs to be deciphered. The latest evidence reveals the critical role of the non-coding RNAs, particularly microRNAs (miRNAs), as vital regulatory elements in cancer.MethodWe performed a miRNAs microarray using paired tissues (tumor and adjacent normal bladder tissue), followed by the validation with qRT-PCR of five selected transcripts. Additional next-generation sequencing investigation established the interconnection among the altered miRNAs and mutated genes. Based on the overlapping between TCGA data and data obtained in the study, we focused on the systematic identification of altered miRNAs and genes mutated involved in bladder cancer tumorigenesis and progression.ResultsBy overlapping the miRNAs expression data, the two patient cohorts, we identified 18 miRNAs downregulated and, 187 miRNAs upregulated. qRT-PCR validation was completed using a selected panel of two downregulated (miR-139-5p and miR-143-5p) and three up-regulated miRNAs (miR-141b, miR-200 s or miR-205). Altered miRNAs patterns are interrelated to bladder tumorigenesis, allowing them to be used for the development of novel diagnostic and prognostic biomarkers. Three EMT-related upregulated miRNAs have an essential role in the molecular mechanisms, specifically key processes underlying tumorigenesis, invasion and metastasis. Using the Ampliseq Cancer Panel kit and Ion Torrent PGM Next-Generation Sequencing an increased mutation rate for TP53, FGFR3, KDR, PIK3CA and ATM were observed, but the mutational status for only TP53 was correlated to the survival rate. The miRNAs pattern, along with the gene mutation pattern attained, can assist for better patient diagnosis.ConclusionThis study thereby incorporates miRNAs as critical players in bladder cancer prognosis, where their altered gene expression profiles have a critical biological function in relationship with tumor molecular phenotype. The miRNA-mRNA regulatory networks identified in BC are ripe for exploitation as biomarkers or targeted therapeutic strategies.

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Section: Discussionmentioning

confidence: 99%

Connecting the dots between different networks: miRNAs associated with bladder cancer risk and progression

Braicu

Buiga

Cojocneanu

et al. 2019

J Exp Clin Cancer Res

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“…Along with the aging population and the increasing age of surgical patients, the issue of perioperative complications and its costs gains importance (15). Despite the developing technologies, follow-up, and treatment of patients with invasive methods, bladder cancer still continues to be one of the expensive malignancies (16). There are relatively few articles on bibliometric analysis of urologic disorders; and one in bladder cancer.…”

Section: Discussionmentioning

confidence: 99%

Bibliometric Analysis of the Bladder Cancer Publications Between 1975-2018

Sungur

Çalışkan²,

Lokman

et al. 2020

Harran Üniversitesi Tıp Fakültesi Dergisi

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Background: Bladder cancer is one of the major health problems studied over the years. Emerging technologies on follow-up and treatment of bladder cancer increased scientific literature worldwide. Despite this, there is limited data about the bibliometric analysis of bladder cancer. Materials and Methods: The bibliometric analysis of bladder cancer publications between 1975-2018 was performed by using the Thomson Reuters Web of Science database. The VOSviewer software tool was used for arranging and setting bibliometric networks. Results: The United States of America ranked first in this field with 7963 publications and 28.3% of the world generation, followed by the Peoples Republic of China and Germany. The University of Texas System is the leading institute in published documents with 1081 papers and 3.8% of the total literature. 15.9% of the papers were printed in The Journal of Urology with 4483 manuscripts, followed by European Urology (n=867, 3.0%) and Urology (n=810, 2.8%). Conclusions: There are relatively few articles on bibliometric analysis of urologic disorders and bladder cancer. Advanced evaluation parameters have to be defined for a more confident assessment of scientific work. Bibliometric analyses can guide young clinicians in investigating new studies.

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“…These last two techniques are routinely used as follow-up methods. As a consequence, novel non-invasive biomarkers are being explored to early and accurately diagnose and monitor BC patients in order to avoid the performance of this aggressive technique while reaching or even exceeding the sensitivity and specificity of cystoscopy [6]. Urine represents the most accessible source of biological markers for the analysis of BC and other urological tumors since it is rapid and easily obtained by the patient, avoids patient discomfort and potential complications from an invasive procedure, is available in copious amounts and re-sampling is easily achievable [7].…”

Section: Introductionmentioning

confidence: 99%

Identification of miR-29c-3p as a Robust Normalizer for Urine microRNA Studies in Bladder Cancer

et al. 2020

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Bladder cancer (BC) is among the most frequent malignancies worldwide, being the most expensive cancer to treat and monitor and the most lethal urological cancer. Urine microRNAs (miRNAs) have been proposed as novel non-invasive biomarkers to early diagnose and monitor BC patients in order to avoid the performance of current aggressive diagnostic techniques. However, huge discrepancies arise among studies mainly due to the lack of standardization in the normalization, a crucial step in all miRNA studies. Our aim was to identify the best miRNA normalizer for miRNA studies in urine of BC patients. We evaluated the performance of 110 candidate miRNAs in urine of 35 BC patients and 15 healthy controls by Real Time quantitative Polymerase Chain Reaction (RT-qPCR) followed by a stability analysis with RefFinder. In this screening stage, miR-29c-3p arose as the most stably expressed miRNA in BC and controls, with a good expression level. Stability of miR-29c-3p expression was validated in an independent cohort of 153 BC patients and 57 controls. Finally, we evaluated the robustness of miR-29c-3p as normalizer in the expression study of miR-200c-3p, a potential diagnostic marker for BC. We propose miR-29c-3p as a normalizer for miRNA studies in BC urine. This is the first study that characterizes a reliable normalizer that may allow the comparison of future urine miRNA studies as non-invasive biomarkers for BC diagnosis and monitoring.

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New Insights in Bladder Cancer Diagnosis: Urinary miRNAs and Proteins

Cited by 19 publications

References 86 publications

Connecting the dots between different networks: miRNAs associated with bladder cancer risk and progression

Connecting the dots between different networks: miRNAs associated with bladder cancer risk and progression

Bibliometric Analysis of the Bladder Cancer Publications Between 1975-2018

Identification of miR-29c-3p as a Robust Normalizer for Urine microRNA Studies in Bladder Cancer

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