New insights in Russula subsect. Rubrinae: phylogeny and the quest for synapomorphic characters

Caboň, Miroslav; Eberhardt, Ursula; Looney, Brian P.; Hampe, Felix; Kolařík, Miroslav; Jančovičová, Soňa; Verbeken, Annemieke; Adamčík, Slavomír

doi:10.1007/s11557-017-1322-0

Cited by 35 publications

(21 citation statements)

References 22 publications

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“…The mtSSU region was amplified using the primer pair MS1 and MS2 (White et al 1990). The rpb2 was amplified using the primers A-Russ-F-frpb2-7CR (Matheny 2005, Caboň et al 2017. All three molecular markers were amplified with Hot Start Firepol Polymerase (Solis Biodyne, Tartu, Estonia) using cycling protocols according to Caboň et al (2017).…”

Section: Molecular Analysismentioning

confidence: 99%

“…The rpb2 was amplified using the primers A-Russ-F-frpb2-7CR (Matheny 2005, Caboň et al 2017. All three molecular markers were amplified with Hot Start Firepol Polymerase (Solis Biodyne, Tartu, Estonia) using cycling protocols according to Caboň et al (2017). The PCR products were purified using Exo-Sap enzymes (Thermo Fisher Scientific, Wilmington, DE) or the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany).…”

Section: Molecular Analysismentioning

confidence: 99%

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Phylogenetic study documents different speciation mechanisms within the Russula globispora lineage in boreal and arctic environments of the Northern Hemisphere

Caboň

Saba

et al. 2019

IMA Fungus

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The Russula globispora lineage is a morphologically and phylogenetically well-defined group of ectomycorrhizal fungi occurring in various climatic areas. In this study we performed a multi-locus phylogenetic study based on collections from boreal, alpine and arctic habitats of Europe and Western North America, subalpine collections from the southeast Himalayas and collections from subtropical coniferous forests of Pakistan. European and North American collections are nearly identical and probably represent a single species named R. dryadicola distributed from the Alps to the Rocky Mountains. Collections from the southeast Himalayas belong to two distinct species: R. abbottabadensis sp. nov. from subtropical monodominant forests of Pinus roxburghii and R. tengii sp. nov. from subalpine mixed forests of Abies and Betula. The results suggest that speciation in this group is driven by a climate disjunction and adaptation rather than a host switch and geographical distance.

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Section: Molecular Analysismentioning

confidence: 99%

Section: Molecular Analysismentioning

confidence: 99%

Phylogenetic study documents different speciation mechanisms within the Russula globispora lineage in boreal and arctic environments of the Northern Hemisphere

Caboň

Saba

et al. 2019

IMA Fungus

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“…We targeted five DNA regions: (1) the internal transcribed spacer regions of nuclear ribosomal DNA (ITS); (2) partial large subunit of nuclear ribosomal DNA (LSU); (3) partial mitochondrial small subunit of ribosomal DNA (mtSSU); (4) the region between domains six and seven of the nuclear gene encoding the second largest subunit of RNA polymerase II ( rpb2 ); (5) part of the translation elongation factor 1-alpha ( tef1α ). The following primer pairs were used for the DNA amplifications: ITS1F–ITS4 for ITS [ 22 , 23 ], LR0R–LR5 for LSU [ 24 ], MS1–MS2 for mtSSU [ 22 ], A-Russ-F–frpb2-7CR for rpb2 [ 25 , 26 ], EF1-983F–EF1-1567R for tef1α [ 27 ]. All molecular markers were amplified with 5× HOT FIREPol ® Blend Master Mix (Solis BioDyne, Tartu, Estonia) applying PCR conditions of Caboň et al [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…The following primer pairs were used for the DNA amplifications: ITS1F–ITS4 for ITS [ 22 , 23 ], LR0R–LR5 for LSU [ 24 ], MS1–MS2 for mtSSU [ 22 ], A-Russ-F–frpb2-7CR for rpb2 [ 25 , 26 ], EF1-983F–EF1-1567R for tef1α [ 27 ]. All molecular markers were amplified with 5× HOT FIREPol ® Blend Master Mix (Solis BioDyne, Tartu, Estonia) applying PCR conditions of Caboň et al [ 26 ]. The PCR products were purified using Exo-Sap enzymes (Thermo Fisher Scientific, Wilmington, Germany) and sequenced at SeqMe sequencing company (Dobříš, Czech Republic).…”

Section: Methodsmentioning

confidence: 99%

Description of the Fifth New Species of Russula subsect. Maculatinae from Pakistan Indicates Local Diversity Hotspot of Ectomycorrhizal Fungi in Southwestern Himalayas

Kiran

Caboň

Senko

et al. 2021

Life

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Russula subsect. Maculatinae is morphologically and phylogenetically well-defined lineage of ectomycorrhizal fungi associated with arctic, boreal, temperate and Mediterranean habitats of Northern Hemisphere. Based on phylogenetic distance among species, it seems that this group diversified relatively recently. Russula ayubiana sp. nov., described in this study, is the fifth in the group known from relatively small area of northern Pakistan situated in southwestern Himalayas. This is the highest known number of agaric lineage members from a single area in the world. This study uses available data about phylogeny, ecology, and climate to trace phylogenetic origin and ecological preferences of Maculatinae in southwestern Himalayas. Our results suggest that the area has been recently colonised by Maculatinae members migrating from various geographical areas and adapting to local conditions. We also discuss the perspectives and obstacles in research of biogeography and ecology, and we propose improvements that would facilitate the integration of ecological and biogeographical metadata from the future taxonomic studies of fungi in the region.

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“…There are relatively fewer researches in which multiple genes were analysed, e.g. ITS, mtSSU, nLSU and rpb2 in Li et al (2010b), ITS, nLSU and rpb2 in Park et al (2013), ITS and nLSU in Park et al (2014), ITS, rpb2, atp6, cox3 and chsi in Cao et al (2013) and ITS, mtSSU and rpb2 in Caboň et al (2017). In the present study, six genes, namely nLSU (28S), ITS, tef-1α, mtSSU, rpb1, and rpb2, which have been widely analysed in molecular phylogeny, were selected as candidate biomarkers.…”

Section: Introductionmentioning

confidence: 99%

A preliminary DNA barcode selection for the genus Russula (Russulales, Basidiomycota)

Zhao

Zhang

et al. 2018

Mycology

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Russula is a worldwid genus which has a high species diversity . Aiming accurate and rapid species identification, candidate genes nLSU (28S), ITS, tef-1α, mtSSU, rpb1, and rpb2, were analysed as potential DNA barcodes. This analysis included 433 sequences from 38 well-circumscribed Russula species of eight subgenera. Two vital standards were analysed for success species identification using DNA barcodes, specifically inter-and intra-specific variations together with the success rates of PCR amplification and sequencing. Although the gap between inter-and intra-specific variations was narrow, ITS met the qualification standards for a target DNA barcode. Overlapping inter-and intra-specific pairwise distances were observed in nLSU, tef-1α, mtSSU, and rpb2. The success rates of PCR amplification and sequencing in mtSSU and rpb1 were lower than those of others. Gene combinations were also investigated for resolution of species recognition. ITS-rpb2 was suggested as the likely target DNA barcode for Russula, owing to the two viatal standards above. Since nLSU has the lowest minimum of inter-specific variation, and tef-1α has the highest overlap between intra-and inter-species variations among the candidate genes, they are disqualified from the selection for DNA barcode of Russula. ARTICLE HISTORY

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New insights in Russula subsect. Rubrinae: phylogeny and the quest for synapomorphic characters

Cited by 35 publications

References 22 publications

Phylogenetic study documents different speciation mechanisms within the Russula globispora lineage in boreal and arctic environments of the Northern Hemisphere

Phylogenetic study documents different speciation mechanisms within the Russula globispora lineage in boreal and arctic environments of the Northern Hemisphere

Description of the Fifth New Species of Russula subsect. Maculatinae from Pakistan Indicates Local Diversity Hotspot of Ectomycorrhizal Fungi in Southwestern Himalayas

A preliminary DNA barcode selection for the genus Russula (Russulales, Basidiomycota)

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