New insights into auxin metabolism in Bradyrhizobium japonicum

Torres, Daniela; Benavidez, Iliana; Donadío, Florencia; Mongiardini, Elías J.; Rosas, Susana B.; Spaepen, Stijn; Vanderleyden, Jozef; Pěnčík, Aleš; Novák, Ondřej; Strnad, Miroslav; Frébortová, Jitka; Cassán, Fabricio

doi:10.1016/j.resmic.2018.04.002

Cited by 36 publications

(34 citation statements)

References 43 publications

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“…; Torres et al . ). IAA degradation in B. phytofirmans PsJN plays an important role in promoting plant growth and might affect efficient colonization in the rhizosphere (Zúñiga et al .…”

Section: Discussionmentioning

confidence: 97%

“…; Torres et al . ) for which the understanding of the whole IAA metabolism, besides to the IAA biosynthesis, clearly allowed to establish a more detailed model. Therefore, our main objective was to analyse the global metabolism of IAA in three strains of A. brasilense and propose a renewed model to explain IAA metabolism in one of the most studied phytostimulatory bacteria around the world.…”

Section: Introductionmentioning

confidence: 99%

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New insights into indole-3-acetic acid metabolism inAzospirillum brasilense

et al. 2018

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“…; Torres et al . ). IAA degradation in B. phytofirmans PsJN plays an important role in promoting plant growth and might affect efficient colonization in the rhizosphere (Zúñiga et al .…”

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

New insights into indole-3-acetic acid metabolism inAzospirillum brasilense

et al. 2018

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“…The predicted and annotated gene sequences obtained from B. pyrrocinia JK-SH007 genome were analyzed by similarity analysis with KEGG (Kyoto Encyclopedia of Genes and Genomes) (Kanehisa et al, 2012) and NCBI (National Center for Biotechnology Information), and then each gene was allocated to the KEGG pathway maps of Trp metabolism and IAA biosynthesis. Based on this analysis, the whole IAA biochemical pathways was proposed and compared with the metabolite spectrum obtained by B. pyrrocinia JK-SH007 in vitro (Torres et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Indole-3-Acetic Acid in Burkholderia pyrrocinia JK-SH007: Enzymatic Identification of the Indole-3-Acetamide Synthesis Pathway

et al. 2019

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Burkholderia pyrrocinia JK-SH007 is a plant growth-promoting bacteria (PGPB), that can promote the growth of poplar and other trees, and, production of the plant hormone indole-3-acetic acid (IAA) is one of the reasons for this effect. Therefore, the aims of this study were to evaluate the effect of the external environment on the synthesis of IAA by B. pyrrocinia JK-SH007 and to perform a functional analysis of its IAA synthesis pathway. In this study, IAA and its synthetic intermediates indole-3-acetamide (IAM), indole-3-pyruvic acid (IPyA), tryptamine (TAM), and indole-3-acetonitrile (IAN) were detected in B. pyrrocinia JK-SH007 fermentation broth by high-performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS), and these indolic compounds were also found in the cell-free extraction of B. pyrrocinia JK-SH007, but the genomic analysis of B. pyrrocinia JK-SH007 indicated that IAA biosynthesis was mainly through the IAM and TAM pathways. The effects of L-tryptophan (L-Trp), temperature and pH on the synthesis of IAA were investigated, and the results showed that L-Trp exerted a significant effect on IAA synthesis and that 37°C and pH 7 were the optimal conditions IAA production by B. pyrrocinia JK-SH007. In addition, the protein expression of tryptophan 2-monooxygenase and indoleacetamide hydrolase, which are the key enzymes of the indole acetamide-mediated IAA synthesis pathway, was analyzed, and their activity was verified by substrate feeding experiments. The results revealed the existence of an IAA synthesis pathway mediated by IAM and indicated that this pathway plays a role in B. pyrrocinia JK-SH007. This study lays the foundation for further exploration of the specific pathway and mechanism of IAA synthesis in B. pyrrocinia JK-SH007.

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“…Since improved root growth also has positive effects on plant growth, many efforts have been made to discover auxin-producing capacity in rhizosbacteria. Many previous studies have reported various types of bacterial species with this ability, such as Azospirillum [ 5 ], Agrobacterium [ 6 ], Bacillus [ 7 ], Bradyrhizobium [ 8 ], Enterobacter [ 9 ], Erwinia [ 10 ], Pseudomonas [ 11 ], and Rhizobium [ 12 ]. Other bacteria can stimulate plants to produce more auxins [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

A highly efficient auxin-producing bacterial strain and its effect on plant growth

Park¹,

Kim²,

Hong³

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Various bacteria promote plant root growth in the rhizosphere, as a measure of securing and enlarging their ecological niche. These interactions are mediated by plant growth regulators (PGRs) such as auxin, and indole-3-acetic acid (IAA) is one of the physiologically active auxin. In this study, we isolated an unusual bacterial strain from food process waste with high efficiency and demonstrated its effects on plant rooting and early-stage growth. Results The efficiency of this bacterial strain in producing IAA was 16.6 mg/L/h in Luria-Bertani broth containing 0.05% l-tryptophan (Trp) at room temperature (24 ± 2 °C). Its IAA production was highly dependent on the presence of precursor, Trp. This bacterium was identified as Ignatzschineria sp. by 16S rDNA sequencing. Its bacterial culture supernatant (BCS) enhanced plant root initiation, root growth, and plant growth in the early stages. The root mass formed BCS-treated in apple mint cuttings was twofold of that formed in the control. The root number and length were 46% and 18% higher, respectively, in BCS-treated chrysanthemum cuttings than in the control. Conclusions These results show that the BCS of Ignatzschineria sp. CG20001 isolate obtained in this study can be used for agricultural applications. In addition, the novelty of this strain makes it a valuable genetic resource for biotechnological applications.

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New insights into auxin metabolism in Bradyrhizobium japonicum

Cited by 36 publications

References 43 publications

New insights into indole-3-acetic acid metabolism inAzospirillum brasilense

New insights into indole-3-acetic acid metabolism inAzospirillum brasilense

Indole-3-Acetic Acid in Burkholderia pyrrocinia JK-SH007: Enzymatic Identification of the Indole-3-Acetamide Synthesis Pathway

A highly efficient auxin-producing bacterial strain and its effect on plant growth

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