New insights into the genetic and metabolic diversity of thiocyanate-degrading microbial consortia

Watts, Mathew P.; Moreau, John W.

doi:10.1007/s00253-015-7161-5

Cited by 32 publications

(25 citation statements)

References 54 publications

(107 reference statements)

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“…Microbial decomposition of organic sulfur compounds can also produce COS through a range of different metabolic pathways, including the oxidation of carbon disulphide and thiosulfate [28,29], thiocyanate (SCN − ; [30]), and isothiocyanate [31]. Currently, the SCN − pathway is the most popular hypothesis, as it has been demonstrated in a number of different microorganisms [30,[66][67][68][69][70]. In addition, Kelly et al [71] showed that bacteria oxidising SCN − can use NO 3 − as an oxidant for their N supply, whilst Broman et al [72] further demonstrated that the consumption of SCN − was correlated to NO 3 − consumption in continuous culture bioreactors.…”

Section: Mechanisms Promoting the Production Of Cosmentioning

confidence: 99%

Nitrogen Fertilization Reduces the Capacity of Soils to Take up Atmospheric Carbonyl Sulphide

et al. 2018

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Soils are an important carbonyl sulphide (COS) sink. However, they can also act as sources of COS to the atmosphere. Here we demonstrate that variability in the soil COS sink and source strength is strongly linked to the available soil inorganic nitrogen (N) content across a diverse range of biomes in Europe. We revealed in controlled laboratory experiments that a one-off addition of ammonium nitrate systematically decreased the COS uptake rate whilst simultaneously increasing the COS production rate of soils from boreal and temperate sites in Europe. Furthermore, we found strong links between variations in the two gross COS fluxes, microbial biomass, and nitrate and ammonium contents, providing new insights into the mechanisms involved. Our findings provide evidence for how the soil–atmosphere exchange of COS is likely to vary spatially and temporally, a necessary step for constraining the role of soils and land use in the COS mass budget.

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Section: Mechanisms Promoting the Production Of Cosmentioning

confidence: 99%

Nitrogen Fertilization Reduces the Capacity of Soils to Take up Atmospheric Carbonyl Sulphide

et al. 2018

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“…Microbial decomposition of organic sulfur compounds can also produce COS through a range of different metabolic pathways, including the oxidation of carbon disulphide and thiosulfate [28,29], thiocyanate (SCN -; [30]) and isothiocyanate [31]. Currently, the SCNpathway is the most popular hypothesis as it has been demonstrated in a number of different microorganisms [30,[66][67][68][69][70]. In addition, Kelly et al [71] showed that bacteria oxidising SCNcan use NO3 -as an oxidant for their N supply whilst Broman et al [72] further demonstrated that the consumption of SCNwas correlated to NO3 -consumption in continuous culture bioreactors.…”

Section: Mechanisms Promoting the Production Of Cosmentioning

confidence: 99%

Nitrogen Fertilization Reduces the Capacity of Soils to take up Atmospheric Carbonyl Sulphide

Kaisermann¹,

Jones²,

Wohl³

et al. 2018

Preprint

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Soils are an important COS sink. However they can also act as sources of COS to the atmosphere. Here we demonstrate that variability in the soil COS sink and source strength is strongly linked to available soil inorganic nitrogen (N) content across a diverse range of biomes in Europe. We revealed in controlled laboratory experiments that N fertilisation simultaneously decreases the COS sink strength of soils while increasing the COS production rate. Furthermore, we found strong links between variations in the two gross COS fluxes, microbial biomass and nitrate and ammonium contents, providing new insights into the mechanisms involved. Our findings provide evidence for how soil-atmosphere exchange of COS is likely to vary spatially and temporally, a necessary step for constraining the role of soils and land use in the COS mass budget.

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“…In another recent study, laboratory bioreactors were inoculated from mine tailings and the autotrophically supported consortia found to breakdown SCN - [10]. The currently known mechanisms for thiocyanate degradation are reviewed by Watts and Moreau [11].…”

Section: Introductionmentioning

confidence: 99%

Thiocyanate and organic carbon inputs drive convergent selection for specific autotrophicAfipiaandThiobacillusstrains within complex microbiomes

Huddy

Sachdeva

Kadzinga

et al. 2020

Preprint

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Thiocyanate (SCN -) contamination threatens aquatic ecosystems and pollutes vital fresh water supplies. SCNdegrading microbial consortia are commercially deployed for remediation, but the impact of organic amendments on selection within SCNdegrading microbial communities has not been investigated. Here, we tested whether specific strains capable of degrading SCNcould be reproducibly selected for based on SCNloading and the presence or absence of added organic carbon. Complex microbial communities derived from those used to treat SCNcontaminated water were exposed to systematically increased input SCN concentrations in molasses-amended and -unamended reactors and in reactors switched to unamended conditions after establishing the active SCNdegrading consortium. Five experiments were conducted over 790 days and genomeresolved metagenomics was used to resolve community composition at the strain level. A single Thiobacillus strain proliferated in all reactors at high loadings. Despite the presence of many Rhizobiales strains, a single Afipia variant dominated the molasses-free reactor at moderately high loadings. This strain is predicted to breakdown SCNusing a novel thiocyanate dehydrogenase, oxidize resulting reduced sulfur, degrade product cyanate (OCN − ) to ammonia and CO2 via cyanase, and fix CO2 via the Calvin-Benson-Bassham cycle. Removal of molasses from input feed solutions reproducibly led to dominance of this strain. Neither this Afipia strain nor the thiobacilli have the capacity to produce cobalamin, a function detected in low abundance community members. Although sustained by autotrophy, reactors without molasses did not stably degrade SCNat high loading rates, perhaps due to loss of biofilm-associated niche diversity. Overall, convergence in environmental conditions led to convergence in the strain composition, although reactor history also impacted the trajectory of community compositional change.

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New insights into the genetic and metabolic diversity of thiocyanate-degrading microbial consortia

Cited by 32 publications

References 54 publications

Nitrogen Fertilization Reduces the Capacity of Soils to Take up Atmospheric Carbonyl Sulphide

Nitrogen Fertilization Reduces the Capacity of Soils to Take up Atmospheric Carbonyl Sulphide

Nitrogen Fertilization Reduces the Capacity of Soils to take up Atmospheric Carbonyl Sulphide

Thiocyanate and organic carbon inputs drive convergent selection for specific autotrophicAfipiaandThiobacillusstrains within complex microbiomes

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