New insights on the regulatory network of drought-responsive key genes in Arabidopsis thaliana

Arjmand, Maryam Pasandideh; Lahiji, Habibollah Samizadeh; Golfazani, Mohammad Mohsenzadeh; Biglouei, Mohammad Hassan

doi:10.1007/s10709-022-00177-3

Cited by 12 publications

(5 citation statements)

References 82 publications

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“…We found by comparison that 19 of 87 BR-downregulated but PLT-activated genes were upregulated by drought, while 68 were downregulated by drought; in addition, 87 of 143 BR-upregulated but PLT-suppressed genes were upregulated by drought, while 56 were downregulated by drought ( Figure 6D ; Supplementary Figure S3 , and Supplementary Table S4 ). Among these were several genes that are known to regulate the plant drought response, such as CIPK3 ( Sanyal et al., 2017 ), RCI2A ( Medina et al., 2005 ), PIP1;5 ( Alexandersson et al., 2010 ), ERF61 ( Bano et al., 2022 ), and WRKY60 ( Chen et al., 2010 ; Arjmand et al., 2023 ). Through qRT−PCR, these genes were indeed found to be repressed in PLT1-OX and PLT2-OX ( Figure 7 ).…”

Section: Resultsmentioning

confidence: 99%

The BR signaling pathway regulates primary root development and drought stress response by suppressing the expression of PLT1 and PLT2 in Arabidopsis thaliana

Zhao

Gao

et al. 2023

Front. Plant Sci.

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IntroductionWith the warming global climate, drought stress has become an important abiotic stress factor limiting plant growth and crop yield. As the most rapidly drought-sensing organs of plants, roots undergo a series of changes to enhance their ability to absorb water, but the molecular mechanism is unclear.Results and methodsIn this study, we found that PLT1 and PLT2, two important transcription factors of root development in Arabidopsis thaliana, are involved in the plant response to drought and are inhibited by BR signaling. PLT1- and PLT2-overexpressing plants showed greater drought tolerance than wild-type plants. Furthermore, we found that BZR1 could bind to the promoter of PLT1 and inhibit its transcriptional activity in vitro and in vivo. PLT1 and PLT2 were regulated by BR signaling in root development and PLT2 could partially rescue the drought sensitivity of bes1-D. In addition, RNA-seq data analysis showed that BR-regulated root genes and PLT1/2 target genes were also regulated by drought; for example, CIPK3, RCI2A, PCaP1, PIP1;5, ERF61 were downregulated by drought and PLT1/2 but upregulated by BR treatment; AAP4, WRKY60, and AT5G19970 were downregulated by PLT1/2 but upregulated by drought and BR treatment; and RGL2 was upregulated by drought and PLT1/2 but downregulated by BR treatment.DiscussionOur findings not only reveal the mechanism by which BR signaling coordinates root growth and drought tolerance by suppressing the expression of PLT1 and PLT2 but also elucidates the relationship between drought and root development. The current study thus provides an important theoretical basis for the improvement of crop yield under drought conditions.

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Section: Resultsmentioning

confidence: 99%

The BR signaling pathway regulates primary root development and drought stress response by suppressing the expression of PLT1 and PLT2 in Arabidopsis thaliana

Zhao

Gao

et al. 2023

Front. Plant Sci.

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“…The WRKY9 and WRKY40 TFs are known as central repressors of ABA signaling during A. thaliana seed germination and seedling growth (Wang et al, 2021) and of ROS and stress signaling (Shin and Schachtman, 2004;Van Aken et al, 2013;Arjmand et al, 2023). In Ae.…”

Section: Resetting Of Maturation and Dormancy Genes Tfs And Seed-seed...mentioning

confidence: 99%

Aethionema arabicum dimorphic seed trait resetting during transition to seedlings

Arshad,

Steinbrecher,

Wilhelmsson

et al. 2024

Front. Plant Sci.

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The transition from germinating seeds to emerging seedlings is one of the most vulnerable plant life cycle stages. Heteromorphic diaspores (seed and fruit dispersal units) are an adaptive bet-hedging strategy to cope with spatiotemporally variable environments. While the roles and mechanisms of seedling traits have been studied in monomorphic species, which produce one type of diaspore, very little is known about seedlings in heteromorphic species. Using the dimorphic diaspore model Aethionema arabicum (Brassicaceae), we identified contrasting mechanisms in the germination responses to different temperatures of the mucilaginous seeds (M+ seed morphs), the dispersed indehiscent fruits (IND fruit morphs), and the bare non-mucilaginous M− seeds obtained from IND fruits by pericarp (fruit coat) removal. What follows the completion of germination is the pre-emergence seedling growth phase, which we investigated by comparative growth assays of early seedlings derived from the M+ seeds, bare M− seeds, and IND fruits. The dimorphic seedlings derived from M+ and M− seeds did not differ in their responses to ambient temperature and water potential. The phenotype of seedlings derived from IND fruits differed in that they had bent hypocotyls and their shoot and root growth was slower, but the biomechanical hypocotyl properties of 15-day-old seedlings did not differ between seedlings derived from germinated M+ seeds, M− seeds, or IND fruits. Comparison of the transcriptomes of the natural dimorphic diaspores, M+ seeds and IND fruits, identified 2,682 differentially expressed genes (DEGs) during late germination. During the subsequent 3 days of seedling pre-emergence growth, the number of DEGs was reduced 10-fold to 277 root DEGs and 16-fold to 164 shoot DEGs. Among the DEGs in early seedlings were hormonal regulators, in particular for auxin, ethylene, and gibberellins. Furthermore, DEGs were identified for water and ion transporters, nitrate transporter and assimilation enzymes, and cell wall remodeling protein genes encoding enzymes targeting xyloglucan and pectin. We conclude that the transcriptomes of seedlings derived from the dimorphic diaspores, M+ seeds and IND fruits, undergo transcriptional resetting during the post-germination pre-emergence growth transition phase from germinated diaspores to growing seedlings.

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“…Among them, some proteins were reported to have unique functions, including the MEE5 gene, which was associated with embryo development and the development of the female gametophyte [50,51]. In addition, LSM2 [52] and RAP74 [53] regulate transcript splicing and thus influence plant development, and STZ [54] was related to drought stress resistance. These results suggested that the expression bias of the key TFs in the hybrid might be an important factor influencing the global state of transcription and the phenotype.…”

Section: Transcription Factor Analysis Of Non-additive Genesmentioning

confidence: 99%

Transcriptome Shock in Developing Embryos of a Brassica napus and Brassica rapa Hybrid

Zhou,

Zhang,

et al. 2023

IJMS

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Interspecific crosses that fuse the genomes of two different species may result in overall gene expression changes in the hybrid progeny, called ‘transcriptome shock’. To better understand the expression pattern after genome merging during the early stages of allopolyploid formation, we performed RNA sequencing analysis on developing embryos of Brassica rapa, B. napus, and their synthesized allotriploid hybrids. Here, we show that the transcriptome shock occurs in the developing seeds of the hybrids. Of the homoeologous gene pairs, 17.1% exhibit expression bias, with an overall expression bias toward B. rapa. The expression level dominance also biases toward B. rapa, mainly induced by the expression change in homoeologous genes from B. napus. Functional enrichment analysis revealed significant differences in differentially expressed genes (DEGs) related to photosynthesis, hormone synthesis, and other pathways. Further study showed that significant changes in the expression levels of the key transcription factors (TFs) could regulate the overall interaction network in the developing embryo, which might be an essential cause of phenotype change. In conclusion, the present results have revealed the global changes in gene expression patterns in developing seeds of the hybrid between B. rapa and B. napus, and provided novel insights into the occurrence of transcriptome shock for harnessing heterosis.

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New insights on the regulatory network of drought-responsive key genes in Arabidopsis thaliana

Cited by 12 publications

References 82 publications

The BR signaling pathway regulates primary root development and drought stress response by suppressing the expression of PLT1 and PLT2 in Arabidopsis thaliana

The BR signaling pathway regulates primary root development and drought stress response by suppressing the expression of PLT1 and PLT2 in Arabidopsis thaliana

Aethionema arabicum dimorphic seed trait resetting during transition to seedlings

Transcriptome Shock in Developing Embryos of a Brassica napus and Brassica rapa Hybrid

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