New isocratic high-performance liquid chromatographic procedure to assay the anti-sickling compound hydroxyurea in plasma with ultraviolet detection

Iyamu, Efemwonkiekie W.; Roa, P. D.; Kopsombut, Prapaporn; Aguinaga, Maria del Pilar; Turner, Edward L.

doi:10.1016/s0378-4347(98)00020-6

Cited by 19 publications

(15 citation statements)

References 9 publications

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“…As a result, a recovery of over 95% of hydroxyurea was obtained. This recovery was consistent with either freshly prepared samples containing plasma and standard solutions of hydroxyurea or samples stored for months at 4 • C. The analytical method, therefore, was never adversely affected with time by the possible degrading action of urease on hydroxyurea as reported by Iyamu et al [19].…”

Section: Sample Preparationsupporting

confidence: 87%

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Quantitative analysis of trimethylsilyl derivative of hydroxyurea in plasma by gas chromatography–mass spectrometry

James

Nahavandi

Wyche

et al. 2006

Journal of Chromatography B

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Section: Sample Preparationsupporting

confidence: 87%

“…Unfortunately, the analytical method was not designed for measuring hydroxyurea in biological samples. In contrast, the HPLC method of Iyamu et al [19] measured hydroxyurea in human and mouse plasma. The retention time of hydroxyurea was 12.6 min.…”

Section: Introductionmentioning

confidence: 98%

Quantitative analysis of trimethylsilyl derivative of hydroxyurea in plasma by gas chromatography–mass spectrometry

James

Nahavandi

Wyche

et al. 2006

Journal of Chromatography B

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“…The results of these studies indicate that HU is fairly well absorbed through the gastrointestinal tract of humans, as the plasma HU concentration reached a similar level after intravenous and oral administrations. Previously, we observed that upon intraperitoneal administration of 100 mg/kg HU to normal ICR mice, HU distributed rapidly throughout the circulation and the plasma concentration of HU fell rapidly with a T ½ of 15 min [15]. However, we could not accurately measure the plasma level of HU after an intraperitoneal dose of 10 mg/kg BW to ICR mice due to the lack of an adequate method of analysis.…”

Section: Discussionmentioning

confidence: 91%

“…They reported that upon intraperitoneal administration of 25 mg/kg body weight (BW) of HU, the plasma level of HU was below the limit of detection by the colorimetric method. We previously reported the results of a pharmacokinetic study of HU in ICR (wild-type) mice after a single intraperitoneal administration of HU at 100 mg/kg BW [15]. The plasma HU level was determined by high performance liquid chromatography (HPLC) with an ultraviolet detection system.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic Profile of the Anti-Sickling Hydroxyurea in Wild-Type and Transgenic Sickle Cell Mice

2001

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The pharmacokinetic profile of hydroxyurea (HU) was investigated by measuring the rate of drug disappearance from the plasma in wild-type and transgenic (Tg) sickle cell mice. The absorption and elimination processes of HU exhibited first-order kinetics after intraperitoneal administration of HU at 10, 50, 100 or 200 mg/kg body weight (BW). The dosage had a marked effect on the pharmacokinetics of HU in the Tg sickle cell mice. Although the area under the plasma concentration curve (AUC) increased in direct proportion with the HU dose in the wild-type mice, the AUC increased to a much greater extent at higher doses in the Tg sickle cell mice. In the Tg sickle cell mice, there was a considerable increase in the mean residence time (MRT) and a significant reduction in the apparent clearance (CL/F) at HU dose ≧100 mg/kg BW, when compared to the lower doses. At an HU dose of 200 mg/kg BW, the CL/F in the Tg sickle cell mice was reduced by about 50% of the value obtained at a dose of 10 mg/kg BW. This phenomenon was not noticeable in the wild-type mice. The MRT value in the wild-type mice at all doses was relatively constant. The steady-state distribution volume of HU in both the wild-type and Tg sickle cell mice was relatively constant at all doses of the drug. The AUC, CL/F, MRT, and terminal half-life values at any given HU dose showed significant differences between the wild-type and Tg sickle cell mice. Following intraperitoneal administration of HU at a dose of 10, 50, 100, or 200 mg/kg BW, the mean percentage of HU excreted in the urine of the wild-type and Tg sickle cell mice over 120 min was 84 ± 6.4% and 50 ± 8.2%, respectively, indicating a significant difference in the amount of HU excreted in urine in the two kinds of mice. The results obtained in this study may be useful in establishing an optimal dose of HU in the treatment and management of patients with sickle cell disease and other hemoglobinopathies.

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“…A more widespread determination of HU is by high-performance liquid chromatography (HPLC) methods. At the exception of the method of Iyamu et al [14] who used ionexclusion, HU is chromatographied on a reversed phase column. The drug is detected underivatized by ultraviolet detection [14] or by electrochemical detection [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Plasma hydroxyurea determined by gas chromatography–mass spectrometry

Kettani¹,

Cotton²,

Gulbis³

et al. 2009

Journal of Chromatography B

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New isocratic high-performance liquid chromatographic procedure to assay the anti-sickling compound hydroxyurea in plasma with ultraviolet detection

Cited by 19 publications

References 9 publications

Quantitative analysis of trimethylsilyl derivative of hydroxyurea in plasma by gas chromatography–mass spectrometry

Quantitative analysis of trimethylsilyl derivative of hydroxyurea in plasma by gas chromatography–mass spectrometry

Pharmacokinetic Profile of the Anti-Sickling Hydroxyurea in Wild-Type and Transgenic Sickle Cell Mice

Plasma hydroxyurea determined by gas chromatography–mass spectrometry

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