New LC-MS/MS method with single-step pretreatment analyzes fat-soluble vitamins in plasma and amniotic fluid

Le, Juan; Yuan, Tengfei; Zhang, Yan; Wang, Shao-Ting; Li, Yan

doi:10.1194/jlr.d087569

Cited by 27 publications

(7 citation statements)

References 25 publications

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“…The retinoid analytic methods described in the literature include (ultra) high-performance liquid chromatography ((U)HPLC) coupled with spectrophotometric (UV-Vis/DAD), spectrofluorimetric (FLD), electrochemical (ECD) detection, mass spectrometry (MS and MS/MS), and also supercritical fluid chromatography (SFC) and immunoassay-based ones, featuring different sensitivities, effectiveness towards different biological matrices, benefits, and limitations. A comparison of these methodologies is provided in Table 2 [ 279 , 280 , 281 , 282 , 283 , 284 , 285 , 286 , 287 , 288 , 289 , 290 , 291 , 292 , 293 , 294 , 295 , 296 , 297 , 298 , 299 , 300 , 301 , 302 , 303 , 304 , 305 , 306 , 307 , 308 , 309 , 310 , 311 , 312 , 313 , 314 , 315 , 316 , 317 , 318 , 319 , 320 , 321 , 322 , 323 , 324 ].…”

Section: Analytical Approaches For Measuring Vitamin a Levelsmentioning

confidence: 99%

“…The sensitivity and background reduction advantage of MS/MS allows for performing analyses of small amounts of tissue and biological samples with more reliable qualitative–quantitative data. Information obtained from MS/MS fragmentation can also identify unknown molecules [ 298 , 299 , 300 , 301 , 302 , 303 ].…”

Section: Analytical Approaches For Measuring Vitamin a Levelsmentioning

confidence: 99%

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Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity

et al. 2021

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Vitamin A is a group of vital micronutrients widely present in the human diet. Animal-based products are a rich source of the retinyl ester form of the vitamin, while vegetables and fruits contain carotenoids, most of which are provitamin A. Vitamin A plays a key role in the correct functioning of multiple physiological functions. The human organism can metabolize natural forms of vitamin A and provitamin A into biologically active forms (retinol, retinal, retinoic acid), which interact with multiple molecular targets, including nuclear receptors, opsin in the retina and, according to the latest research, also some enzymes. In this review, we aim to provide a complex view on the present knowledge about vitamin A ranging from its sources through its physiological functions to consequences of its deficiency and metabolic fate up to possible pharmacological administration and potential toxicity. Current analytical methods used for its detection in real samples are included as well.

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Section: Analytical Approaches For Measuring Vitamin a Levelsmentioning

confidence: 99%

Section: Analytical Approaches For Measuring Vitamin a Levelsmentioning

confidence: 99%

Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity

et al. 2021

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“…The stability of 25(OH)D in plasma was comprehensively studied in our previous work (4). The stability of 25(OH)D-INC was tested with medium-level QC samples.…”

Section: Methods Validationmentioning

confidence: 99%

Acylation derivatization based LC-MS analysis of 25-hydroxyvitamin D from finger-prick blood

Le娟乐¹,

Yuan飞袁腾²,

Geng庆耿嘉³

et al. 2019

Journal of Lipid Research

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Vitamin D metabolite analysis possessed significant clinical value for the pediatric department. However, invasive venipuncture sampling and high blood consumption inflicted much suffering on patients. For alleviation, we carried out a LC-MS method for 25-hydroxyvitamin D quantification in only 3 μl of plasma from the considerably less invasive finger-prick blood samples. To improve sensitivity, acylation on C3-hydroxyl (by isonicotinoyl chloride) rather than Diels-Alder adduction on s-cis-diene structure was for the very first time introduced into vitamin D metabolite derivatization. Compared with the existing derivatization approaches, this novel strategy not only prevented isomer interference, but also exhibited higher reacting throughput. For certification, the methodology was systematically validated and showed satisfying consistency with SRM927a. During clinical application, we found a convincing correlation between 25-hydroxyvitamin D and indirect/total bilirubin in jaundiced newborns. Such an observation indicated that vitamin D supplementation may help to achieve optimal outcomes in neonatal jaundice.

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“…Though the measurement of vitamin D is predominantly performed on blood samples obtained by venipuncture in clinical practice (for diagnostic/therapeutic purposes), for research purposes, vitamin D is measured in other biological matrices, such as urine [ 41 ], tissues [ 52 , 53 ], tissue culture cells, umbilical cord blood [ 54 , 55 , 56 ], finger-prick blood [ 57 ], amniotic fluid [ 58 , 59 ], breastmilk [ 38 ], and synovial fluid [ 19 ]. Table 1 lists vitamin D metabolites that have been detected in different biological matrices in a wide concentration range from a few picograms to dozens of nanograms per milliliter of liquid sample.…”

Section: Vitamin D Determinationmentioning

confidence: 99%

Vitamin D: Current Challenges between the Laboratory and Clinical Practice

Máčová

Bičı́ková

2021

Nutrients

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Vitamin D is a micronutrient with pleiotropic effects in humans. Due to sedentary lifestyles and increasing time spent indoors, a growing body of research is revealing that vitamin D deficiency is a global problem. Despite the routine measurement of vitamin D in clinical laboratories and many years of efforts, methods of vitamin D analysis have yet to be standardized and are burdened with significant difficulties. This review summarizes several key analytical and clinical challenges that accompany the current methods for measuring vitamin D. According to an external quality assessment, methods and laboratories still produce a high degree of variability. Structurally similar metabolites are a source of significant interference. Furthermore, there is still no consensus on the normal values of vitamin D in a healthy population. These and other problems discussed herein can be a source of inconsistency in the results of research studies.

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New LC-MS/MS method with single-step pretreatment analyzes fat-soluble vitamins in plasma and amniotic fluid

Cited by 27 publications

References 25 publications

Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity

Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity

Acylation derivatization based LC-MS analysis of 25-hydroxyvitamin D from finger-prick blood

Vitamin D: Current Challenges between the Laboratory and Clinical Practice

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