New Method for Determining the Sugar Composition of Glycoproteins, Glycolipids, and Oligosaccharides by High-performance Liquid Chromatography

Yasuno, Shoichi; Kokubo, Kazuko; Kamei, Masugu

doi:10.1271/bbb.63.1353

Cited by 74 publications

(52 citation statements)

References 16 publications

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“…It is known that PS from YIT 9018, the parental strain of YIT 9029 which was produced by removing bacteriophage FSW from the YIT 9018 genome (33), contains Rha, Glc, Gal, N-acetylglucosamine (GlcNAc), and Nacetylgalactosamine (GalNAc) (28). We also confirmed the sugar composition of YIT 9029 PS using the ABEE labeling kit (44) and found the presence of Rha, Glc, Gal, and others (data not shown), consistent with the results of Nagaoka et al (28). On the basis of our results, the binding profiles of the strains analyzed are not just determined by the sugar composition of the cell wall PSs, but they are also partly determined by the specificity of lectin binding.…”

Section: Resultssupporting

confidence: 64%

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Lectin Microarray Reveals Binding Profiles of Lactobacillus casei Strains in a Comprehensive Analysis of Bacterial Cell Wall Polysaccharides

Yasuda

Tateno

Hirabayashi

et al. 2011

Appl Environ Microbiol

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The patterns of lectin-binding affinity of most strains were found to be unique. There appears to be two types of lectin-binding profiles: the first is characterized by a few lectins, and the other is characterized by multiple lectins with different specificities. We also showed a dramatic change in the lectin-binding profile of a YIT 9029 derivative with a mutation in the cps1C gene, encoding a putative glycosyltransferase. In conclusion, the developed technique provided a novel strategy for rapid profiling and, more importantly, differentiating numerous bacterial strains with relevance to the biological functions of PS.

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Section: Resultssupporting

confidence: 64%

“…The carbohydrate composition of the PS-PG was analyzed by labeling the sample using an ABEE labeling kit (J-Oil Mills Co. Ltd., Tokyo, Japan), followed by high-performance liquid chromatography analysis using a Honenpak C 18 column (75 mm by 4.6 mm [inner diameter]) (44).…”

Section: Ps-pgmentioning

confidence: 99%

Lectin Microarray Reveals Binding Profiles of Lactobacillus casei Strains in a Comprehensive Analysis of Bacterial Cell Wall Polysaccharides

Yasuda

Tateno

Hirabayashi

et al. 2011

Appl Environ Microbiol

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“…A chromatographic analysis [15][16][17] of sugar components after hydrolysis was performed for qniumucin extracted from different types of jellyfish. As shown in Table 2, the results were rather complicated because the sugar components fluctuated depending on the species of jellyfishes, as well as each fraction of the ion-exchange chromatography.…”

Section: Resultsmentioning

confidence: 99%

“…× 75 mm) from J-Oil Mills Inc., 0.2 M potassium borate buffer (pH 8.9) containing 7% CH 3CN, and a standard kit from J-Oil Mills Inc. containing 11 monosaccharides. 15,16 …”

Section: Methodsmentioning

confidence: 99%

Mucin (Qniumucin), a Glycoprotein from Jellyfish, and Determination of Its Main Chain Structure

et al. 2007

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We extracted a novel glycoprotein, a member of the mucin family, from five species of jellyfish with high yields (1%-3% dry weight, 0.02%-0.1% wet weight) and determined its main chain structure and molecular mass. The glycoprotein contains unique tandem repeats of eight amino acids, of which two threonine residues are probably glycosylated by N-acetyl-D-galactosamine (GalNAc). We named this substance, which is common in jellyfish and similar to the human mucin MUC5AC, "qniumucin" and suggested the utilization of this compound as a new marine resource.

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“…Carbohydrate compositional analysis-Glucose (Glc), galactose (Gal), L-glycero-Dmanno-heptose (Hep), and N-acetylgalactosamine (GalNAc) were analyzed by the method of Fuji et al [18,20]. Briefly, the purified oligosaccharide fraction was hydrolyzed in 2 N HCl and 2 M trifluoroacetic acid for 4 h at 100°C, dried down, and converted to p-aminobenzoic acid ethyl ester (ABEE)-labeled monosaccharides.…”

Section: Structural Analysis Of Lsmentioning

confidence: 99%

Chemical validation of molecular mimicry: interaction of cholera toxin with Campylobacter lipooligosaccharides

et al. 2007

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It is generally believed that molecular mimicry between bacterial lipooligosaccharide (LOS) and nerve glycolipids may play an important pathogenic role in immune-mediated peripheral neuropathy. One of the putative infectious agents is Campylobacter jejuni (C. jejuni). To elucidate the structural basis for the molecular mimicry, we investigated the structure of the lipooligosaccharide (LOS) fraction of C. jejuni, strain HS19, and found that it includes at least two components, characterized as fast-and slow-moving bands (LF and LS) by thin-layer chromatography as revealed by cholera toxin B subunit (Ctxb) overlay. Structural analysis of the oligosaccharide portion of LS established that it had the following structure: Gal-GalNAc-(NeuAc)Gal-Hep-(Glc;PO 3 H)Hep-Kdo. The GM1-like epitope was validated by a terminal tetrasaccharide unit within this structure. On the other hand, analysis of LF revealed an entirely different structure: 1, 4′-bisphosphoryl glucosamine disaccharide N, N'-acylated by 3-(2-hydroxytetracosanoyloxy)octadecanoic acid at 2-and 2′-positions, which is consistent with that of lipid A. No GM1-like epitope was observed in LF. Both LS and LF interacted with Ctxb as demonstrated by TLC-overlay and sucrose density gradient centrifugation. Surprisingly, LF does not have the basic GM1 structure for interacting with Ctxb. Instead, the affinity of LF to Ctxb required that one or both of the phosphate groups be present in the glucosamine disaccharide residue because after alkaline phosphatase treatment the dephosphorylated LF was unable to bind to Ctxb. We conclude that LS is likely the component contributing to GM1-mimicry in autoimmune peripheral neuropathy and that the role of LF is not clear but may be associated with the initial activation of autoreactive T cells.

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New Method for Determining the Sugar Composition of Glycoproteins, Glycolipids, and Oligosaccharides by High-performance Liquid Chromatography

Cited by 74 publications

References 16 publications

Lectin Microarray Reveals Binding Profiles of Lactobacillus casei Strains in a Comprehensive Analysis of Bacterial Cell Wall Polysaccharides

Lectin Microarray Reveals Binding Profiles of Lactobacillus casei Strains in a Comprehensive Analysis of Bacterial Cell Wall Polysaccharides

Mucin (Qniumucin), a Glycoprotein from Jellyfish, and Determination of Its Main Chain Structure

Chemical validation of molecular mimicry: interaction of cholera toxin with Campylobacter lipooligosaccharides

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