New Method for Differentiating Chronic Prostatitis/Chronic Pelvic Pain Syndrome IIIA From IIIB Involving Seminal Macrophages and Monocytes

Aghazarian, Artin; Plas, Eugen; Stancik, Igor; Pflüger, Heinz; Lackner, Jakob

doi:10.1016/j.urology.2011.06.028

Cited by 9 publications

(17 citation statements)

References 25 publications

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“…Evidence has been produced that macrophages play an active role in the inflammatory processes within the male genital tract. High levels of chemokines that induce macrophage chemotaxis are found in ejaculates of patients with chronic inflammatory prostatitis (Penna et al ., ); semen concentrations of macrophages (Aghazarian et al ., ) and macrophages‐derived inflammatory cytokines, such as IL‐6 and IL‐8, are increased in chronic prostatitis (Orhan et al ., ; Penna et al ., ). High seminal concentrations of macrophages‐derived cytokines (IL‐8 and TNF‐α) have been especially detected when leukocytospermia was associated with the presence of pathogens in the ejaculate (Martínez‐Prado & Bermúdez, ).…”

Section: Introductionmentioning

confidence: 99%

2‐arachidonoylglycerol levels are increased in leukocytospermia and correlate with seminal macrophages

et al. 2016

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SUMMARYEvidence has been produced that macrophages can actively generate endocannabinoids (eCBs) in response to inflammatory stimuli. As eCBs are involved in the control of several physiological processes, including reproduction, here, we explored whether seminal levels of the eCBs, N-arachidonoylethanolamine (AEA), and 2-arachidonoylglycerol (2-AG), were higher in the presence of leukocytospermia, and were correlated with semen concentration of macrophages. The content of AEA and 2-AG was measured by high-performance liquid chromatography/mass spectrometry in seminal plasma of ejaculates from 18 leukocytospermic patients (>1 9 10 6 leukocytes/mL) and 21 normozoospermic controls. In the same ejaculates, round cells were phenotyped by flow-cytometry as leukocytes (CD45+), macrophages (CD14+), and activated macrophages (CD14+, HLA-DR+). The levels of 2-AG, but not of AEA, were significantly higher in ejaculates from leukocytospermic patients than in controls and exhibited a significant correlation with semen concentration of macrophages and activated macrophages. Significant associations of 2-AG with macrophages and activated macrophages persisted after adjustment for semen volume and sperm concentration. In conclusion, here we provide evidence that seminal plasma levels of 2-AG are higher in the presence of leukocytospermia, as a marker of macrophages activation. Further studies are warranted to elucidate possible clinical implications.

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Section: Introductionmentioning

confidence: 99%

2‐arachidonoylglycerol levels are increased in leukocytospermia and correlate with seminal macrophages

et al. 2016

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“…Studies have shown that inflammatory cytokines in seminal plasma of CP/CPPS patients are increased significantly, including IL-1, IL-6, IL-8, IL-10, and TNF-a (36). Polymorph nuclear (PMN) elastase in EPS was significantly higher in IIIa compared to IIIb (37,38). Other pathogens than bacteria, including chlamydia, are associated with increased WBC counts and pain severity in men with CP/CPPS.…”

Section: Discussionmentioning

confidence: 99%

A urine-based biomarker for chronic prostatitis/chronic pelvic pain syndrome: a retrospective multi-center study

Liang¹,

Wu²,

Zhang³

et al. 2020

Transl Androl Urol

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Background: Chronic prostatitis (CP) or chronic pelvic pain syndrome (CPPS) is one of the most common diseases in young and middle-aged men, accounting for 30% of outpatient men in urology OPD. There are no definitive diagnostic criteria for CP or CPPS and no accepted therapies that cure the disease.Methods: We identified 372 patients with CP diagnosed from 2015 to 2018 and collect the information of age, routine urinary test, express prostatic secretion (EPS), and NIH-Chronic Prostatitis Symptom Index (NIH-CPSI).Results: Our study proved a correlation between the increase of prostatic exosomal proteins (PSEPs) level and NIH-CPSI scores. Spearman's correlation coefficient showed a significant level correlation between NIH-CPSI and PSEP level (rs=0.194, P=0.0035). In the meantime, the correlation was found between the PSEP level and EPS-white blood cells. Spearman's correlation coefficient showed that there was a significant hierarchical correlation between EPS-white blood cells and PSEP level (rs=0.183, P=0.001).Conclusions: These findings highlight the potential of PSEP is a practical indicator of the symptomatic progression of CP/CPPS. Applications of PSEP assay may guide drug discovery and lead to better treatment to improve the patient's quality of life.All in all, PSEP detection in urine is safe and effective, and it is worthy of further promotion and application in clinical practice.

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“…21 Polymorph nuclear (PMN) elastase in EPS was also shown to be signi cantly higher in IIIa in comparison to that of IIIb. 22,23 The presence of other pathogens than bacteria, such as Chlamydia, is associated with increased WBC counts and pain severity in men with CP/CPPS. Perhaps the most thorough survey of the protein biomarkers came from mass spectrometry of seminal plasma proteome of prostatitis patients.…”

Section: Discussionmentioning

confidence: 99%

A Urine-Based Biomarker for Chronic Prostatitis/Chronic Pelvic Pain Syndrome: A Retrospective Multi-Center Study

Xj¹,

Gw²,

Hu³

et al. 2020

Preprint

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Background There are currently no objective diagnostic criteria for chronic prostatitis or chronic pelvic pain syndrome and no accepted therapies that cure the disease.Methods We identified 372 patients with chronic prostatitis diagnosed from 2015 to 2018 and collect the information of age, routine urinary test, EPS and NIH-CPSI.Results Our study demonstrated a correlation between the increase of PSEP level and NIH-CPSI scores. At meantime, the correlation was found between the PSEP level and EPS-white blood cells.Conclusions These findings highlight the potential of PSEP as a viable indicator of symptomatic progression of CP/CPPS. Applications of PSEP assay may guide drug discovery and lead to the better treatment to improve patient’s quality of life.

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New Method for Differentiating Chronic Prostatitis/Chronic Pelvic Pain Syndrome IIIA From IIIB Involving Seminal Macrophages and Monocytes

Cited by 9 publications

References 25 publications

2‐arachidonoylglycerol levels are increased in leukocytospermia and correlate with seminal macrophages

2‐arachidonoylglycerol levels are increased in leukocytospermia and correlate with seminal macrophages

A urine-based biomarker for chronic prostatitis/chronic pelvic pain syndrome: a retrospective multi-center study

A Urine-Based Biomarker for Chronic Prostatitis/Chronic Pelvic Pain Syndrome: A Retrospective Multi-Center Study

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