New method for fabricating an α‐fetoprotein affinity monolithic polymer array

Abstract

Bisphenol A based epoxy acrylate (BABEA), a commercial UV-curable material, was introduced as a crosslinker for the fabrication of an epoxy-functionalized monolithic polymer array through UV-initiated copolymerization with glycidyl methacrylate as the functional monomer and poly(ethylene glycol) 200 as the porogen. Scanning electron microscopy images showed that the monolithic poly(bisphenol A based epoxy acrylate-co-glycidyl methacrylate) [poly(BABEA-co-GMA)] exhibited a well-controlled skeletal and well-distributed porous structure. The a-fetoprotein (AFP) immunoaffinity monolithic polymer array prepared by the immobilization of AFP on epoxy-functionalized monolithic arrays was used as an immunosensor for chemiluminescent AFP detection. X-ray photoelectron spectroscopy results indicate that the AFP antibody was successfully immobilized on the monolithic poly(BABEA-co-GMA) array. With a noncompetitive immune-response format, the proposed AFP immunoaffinity array was demonstrated as a lowcost, flexible, homogeneous, and stable array for AFP detection.

Immune‐affinity monolithic array with chemiluminescent detection for mycotoxins in barley

“…PPMs have been extensively used as matrices for separating and purifying molecules, owing to the unique characterisation of simple preparation, easy modification, large surface area to volume ratio, and convective mass transmission . An affinity monolith obtained usually by immobilisation of antibodies or aptamers on the surface of the monolith has been used for separating and purifying proteins and cells from complicated samples.…”