New Method for Immobilization of Microbial Cells and its Industrial Application

Chibata, Ichiro; Tosa, Tetsuya; Sato, Tatsuya; Yamamoto, Kenrou; Takata, Isao; Nishida, Yoshihumi

doi:10.1007/978-1-4684-6985-1_66

Cited by 12 publications

(2 citation statements)

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“…Chibata et al (1974dChibata et al ( , 1975cChibata et al ( , 1978a entrapped Streptomyces aureus, Streptomyces griseus, Streptomyces olivaceus, andß. More recently Chibata and co-workers (Chibata et al, 1978c; entrapped Streptomyces phaechromogenes cells in /c-carrageenan gel beads. Some activity loss is likely during immobilization as a result of the cytotoxicity of the polymerizing catalyst and the denaturing of enzyme caused by the monomer (Bagnail, 1978).…”

Section: A Glucose Isomerization In the Starch And Sugar Industrymentioning

confidence: 99%

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Applications of Immobilized Microbial Cells

Linko¹,

Linko²

1983

Immobilized Microbial Cells

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Section: A Glucose Isomerization In the Starch And Sugar Industrymentioning

confidence: 99%

“…Recently an improved process based on the entrapment of E. colt and other microbial cells in κ-carrageenan has been developed (Chibata et al, 1978cSato et al, 1979;Nishida et al, 1979). Stability was improved by treatment with glutaraldehyde.…”

Section: B Biosynthetic Production Of L-aspartic Acidmentioning

confidence: 99%

Applications of Immobilized Microbial Cells

Linko¹,

Linko²

1983

Immobilized Microbial Cells

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Immobilisierung mikrobieller Zellen und deren Nutzung zur Substratwandlung – Eine Literaturstudie. 1. Fortsetzung

Berger¹

1982

Acta Biotechnologica

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Preparation of L‐aspartic acid by means of immobilized Alcaligenes metalcaligenes cells

Vojtšek¹,

Guttrnann²,

Bárta³

et al. 1986

Biotech & Bioengineering

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Two types of biocatalysts based on immobilized cells of Alcaligenes metalcaligenes exhibiting aspartate ammonia-lyase activity (EC 4.3.1.1) were developed for the enzymic preparation of L-aspartic acid from ammonium fumarate. The first type of the biocatalyst consists in individual covalently crosslinked and permeabilized cells(I), while the second type is represented by cell aggregates (II). For the above preparation, biocatalyst I can be used only discontinuously in a mixed reactor. After termination of the reaction between individual cycles of its use, the biocatalyst is returned to the reactor in the form of a highly concentrated cell suspension or paste. Biocatalyst II can be used discontinuously or continuously in a fixed-bed column of the catalyst. The effects of pH, substrate concentration and temperature on the reaction velocity and effectivity of enzymic conversion was investigated. Optimal parameters of the reaction are as follows: pH 8.5, initial substrate concentration, 1.35 mol/L, temperature for discontinuous process, 37 degrees C, and temperature for continuous process, 25 degrees C. Under these conditions the enzymic conversion of substrate to product is quantitative. Under optimal toring conditions, the specific activity of both catalysts does not change within a period of one year. The operational half-life of the biocatalyst II during continuous use in a fixed-bed column of the catalyst under standard reaction conditions depends on the quality of the substrate. The discontinuous preparation of L-asparatic acid with the aid of biocatalyst I and continuous preparation of this product with the aid of biocatalyst II have been verified under pilot-plant conditions.

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New Method for Immobilization of Microbial Cells and its Industrial Application

Cited by 12 publications

References 0 publications

Applications of Immobilized Microbial Cells

Applications of Immobilized Microbial Cells

Immobilisierung mikrobieller Zellen und deren Nutzung zur Substratwandlung – Eine Literaturstudie. 1. Fortsetzung

Preparation of L‐aspartic acid by means of immobilized Alcaligenes metalcaligenes cells

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