New method for investigation of cells and other biological objects in analytical cytology

Bilyi, Olexander I.; Getman, Vasyl B.; Kostyukevych, Sergey A.

doi:10.1117/12.426773

Cited by 5 publications

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“…Similarity of refractive indexes of bacterial cells' cytoplasm and surrounding environment, which respond the criterion of using Reley-Gans'es theory allow to claim that this model is suitable for description of light scattering processes by the microbiological objects. For the estimation of particle sizes distribution a method, which is based on the registration of dissipated light intensity changes is offered 13,14,15 . This method includes sounding of flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding radiation with the explored microbiological objects by detects amplitudes and durations of scattered light impulses.…”

Section: Methodsmentioning

confidence: 99%

The influence of different metal ions on light scattering properties of pattern microbial fuel cells' bacteria Desulfuromonas acetoxidans

et al. 2011

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Microbial fuel cell (MFC) technologies represent the newest approach for generating electricity -bioelectricity generation from biomass using bacteria. Desulfuromonas acetoxidans are aquatic obligatory anaerobic sulfur-reducing bacteria that possess an ability to produce electric current in the processes of organic matter oxidation and Fe 3+ -or Mn 4+ -reduction. These are pattern objects for MFC systems. They could be applied as a highly effective and self-sustaining model of wastewater treatment which contains energy in the form of biodegradable organic matter. But wastewaters contain high concentrations of xenobiotics, such as different heavy metals that have a detrimental effect towards all living organisms. The influence of different concentrations of MnCl 2 ×4H 2 O, FeSO 4 CuSO 4 , CdSO 4 , ZnSO 4 and PbNO 3 on light scattering properties of aquatic D. acetoxidans bacteria on the base of their cells' size distribution and relative content has been investigated by the new method of measurement. The cell distribution curve was in the range of 0.4 -1.4 µm. The most crucial changes of cell concentration dependences, compared with other investigated metal ions, have been observed under the influence of copper ions. The ability of D. acetoxidans bacteria to produce electric current upon the specific cultivation conditions and the influence of Fe 2+ and Mn 2+ has been verified.

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Section: Methodsmentioning

confidence: 99%

The influence of different metal ions on light scattering properties of pattern microbial fuel cells' bacteria Desulfuromonas acetoxidans

et al. 2011

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“…In this work a new method for the estimation of light scattering properties of bacterial cells is offered 6,7,8 . This method includes the sounding of flow suspended bacterial cells by monochromatic coherent light, the registration of cooperative signals of sounding radiation and the explored microbiological objects by detecting amplitudes and duration of scattered light impulses.…”

Section: Methodsmentioning

confidence: 99%

The changes of spectroscopic characteristics of sulfurreducing bacteria Desulfuromonas acetoxidans under the influence of different metal ions

et al. 2011

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Desulfuromonas acetoxidans, which is regarded to the oldest microorganisms that exist in the Earth, are uncoloured gram-negative obligatory anaerobic bacteria that have an ability to reduce S 0 to Н 2 S. This process supports bacteria with sufficient amount of energy which they need for growth. At the same time high concentrations of hydrogen sulfide are very toxic towards all living organisms. Different metal ions that exist in surrounding environment in small concentrations are essential for microorganisms because they support normal functionality of them. But in high concentrations they have a detrimental influence on cell structure and it functions. Srains of D. acetoxidans bacteria that have high toxic metals resistance can neutralize the toxicity of hydrogen sulfide, which is the final product of dissimilative sulfurreduction, and these metals as the result of their particular binding and forming the insoluble precipitations. Light scattering changes and metals accumulation ability of D. acetoxidans bacterial cells under the influence of CuSO 4 , PbNO 3 , ZnSO 4 and CdSO 4 have been investigated. The changes of light scattering characteristics of bacterial D. acetoxidans cells on the base of their size distribution and relative content under the influence of investigated metal salts have been observed by the new method of measurement. Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/26/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx Proc. of SPIE Vol. 8152 81520B-2 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/26/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx Proc. of SPIE Vol. 8152 81520B-6 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/26/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx

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“…Âè-çíà÷åííÿ îñòàííüîãî îïòè÷íèìè ìåòîäàìè, çà ë³òåðàòóðíèìè äàíèìè, ìîaeëèâå â ðàìêàõ òåîð³¿ Ðåëåÿ [5], òåîð³¿ ðîçñ³ÿííÿ Ì³ [6] ³ òåîð³¿ Ðåëåÿ-Ãàíñà [7,8]. Ïîä³áí³ñòü çà çíà÷åííÿì ïî-êàçíèê³â çàëîìëåííÿ öèòîïëàçìè áàêòåð³éíèõ êë³òèí òà íàâêîëèøíüîãî ñåðåäîâèùà, ùî â³ä-ïîâ³äàº êðèòåð³þ çàñòîñîâóâàííÿ òåîð³¿ Ðåëåÿ- , ð³âíå 25,7 õ 10 -5 [10,11]. Öåé ðåçóëüòàò ñâ³ä÷èòü, ùî âåëè÷èíà ñèãíàëó ñâ³òëà, ùî ðîçñ³þºòüñÿ áàêòåð³ÿìè, º íà ï'ÿòü ïîðÿäê³â íèae÷à, çà ³íòåíñèâí³ñòü ïàäàþ÷îãî ïðîìåíÿ, à òîìó ìåòîä ñòàòè÷íîãî ðîçñ³ÿííÿ ñâ³òëà äëÿ äîñë³äaeóâàííÿ áàêòåð³éíèõ êë³òèí ïðàêòè÷íî íå çàñòîñîâóºòüñÿ.…”

Section: âñòóïunclassified

The Influence of Heavy Metals on Light Scattering Properties of Sulfur Cycle Bacteria Desulfuromonas Acetoxidans

Білий¹,

Василів²,

Getman³

et al. 2011

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á-Ëüâ³âñüêèé íàö³îíàëüíèé óí³âåðñèòåò ³ìåí³ ²âàíà Ôðàíêà, á³îëîã³÷íèé ôàêóëüòåò, êàôåäðà ì³êðîá³îëîã³¿, âóë Ãðóøåâñüêîãî 4 ì Ëüâ³â 79005, Óêðà¿íà ÂÏËÈÂ ÂÀAEÊÈÕ ÌÅÒÀË²Â ÍÀ ÑÂ²ÒËÎÐÎÇÑ²ÞÞ×² ÂËÀÑÒÈÂÎÑÒ² ÁÀÊÒÅÐ²É ÖÈÊËÓ Ñ²ÐÊÈ DESULFUROMONAS ACETOXIDANS Î. ². Á³ëèé, Î. Ì. Âàñèë³â, Â. Á. Ãåòüìàí, Ñ. Î. Ãíàòóø Àíîòàö³ÿ. Ó ðîáîò³ äîñë³äaeåíî âïëèâ äåÿêèõ ñîëåé âàaeêèõ ìåòàë³â íà ñâ³òëîðîçñ³þþ÷³ âëàñòèâîñò³ ñ³ðêîâ³äíîâëþâàëüíèõ áàêòåð³é Desulfuromonas acetoxidans. Âñòàíîâëåíî çàëåae-í³ñòü ì³ae êîíöåíòðàö³éíèìè çì³íàìè òà â³äíîñíèì âì³ñòîì êë³òèí ó âèáðàíîìó ä³àïàçîí³ ðîçì³ð³â çà âíåñåííÿ êàäì³é ñóëüôàòó, êóïðóì ñóëüôàòó, öèíê ñóëüôàòó òà ïëþìáóì í³òðàòó.

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New method for investigation of cells and other biological objects in analytical cytology

Cited by 5 publications

References 4 publications

The influence of different metal ions on light scattering properties of pattern microbial fuel cells' bacteria Desulfuromonas acetoxidans

The influence of different metal ions on light scattering properties of pattern microbial fuel cells' bacteria Desulfuromonas acetoxidans

The changes of spectroscopic characteristics of sulfurreducing bacteria Desulfuromonas acetoxidans under the influence of different metal ions

The Influence of Heavy Metals on Light Scattering Properties of Sulfur Cycle Bacteria Desulfuromonas Acetoxidans

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