The changes of spectroscopic characteristics of sulfurreducing bacteria Desulfuromonas acetoxidans under the influence of different metal ions

Vasyliv, Oresta M.; Bilyy, Olexandr I.; Getman, Vasyl B.; Kushkevych, Ivan; Hnatush, S. О.

doi:10.1117/12.892307

Cited by 5 publications

(5 citation statements)

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“…Сульфідогенні бактерії у нішах із низьким окисно-відновним потенціалом, збагачених органічними сполуками, здійснюють відновну трансформацію окиснених сполук сульфуру з утворенням значних кількостей гідроген сульфіду (Richter et al, 2012). Гідроген сульфід, утворений бактеріями, взаємодіє з іонами важких металів (Fe (II), Cu (II), Cd (II), Ni (II), Pb (II), Zn (II)), осаджуючи їх у формі сульфідів, або є сильним відновлюючим агентом, який сприяє редукції металів до більш відновлених форм (White et al, 2000;Frank & Lushnikov, 2006;Wang et al, 2008;Kozlova et al, 2008;Gudz et al, 2011;Vasyliv et al, 2011;Moroz, 2013;Kiran et al, 2017). Осадження сульфідів може здійснюватися сульфідогенною мікробіотою у відстійниках, лагунах, ставках, анаеробних реакторах і відбуватися поза клітинами мікроорганізмів, у клітинах або на поверхні клітин за рН 3-9 (Kuznetsov et al, 2015).…”

Section: вступunclassified

Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds

et al. 2018

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Moroz, O. M., Hnatush, S. O., Tarabas, O. V., Bohoslavets, C. I., Yavorska, G. V., & Borsukevych, B. M. (2018). Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds. Biosystems Diversity, 26(1), 3-10.Due to their high content in natural environments, heavy metals exhibit toxic effects on living organisms, which leads to a decrease in the biological diversity and productivity of ecosystems. In niches with low oxidation reducing potential, sulfate and sulfur reducing bacteria carry out the reducing transformation of oxidized sulfur compounds with the formation of significant amounts of hydrogen sulfide. H 2 S produced by bacteria interacts with metal ions, precipitating them in the form of sulfides. The aim of this work was to investigate the influence of lead, cuprum (II), iron (II) and manganese (II) salts on the production of hydrogen sulfide by bacteria of the Desulfovibrio and Desulfuromonas genera, isolated from Yavorivske Lake, and to evaluate the efficiency of their use for purifying media, enriched with organic compounds, from hydrogen sulfide and heavy metals. The content of heavy metal ions in the water of Yavorivske Lake was determined by the spectrophotometric method. The bacteria were grown for 10 days at 30 °C in the Kravtsov-Sorokin medium under anaerobic conditions. To study the influence of metal ions on bacteria growth and their H 2 S production, cells were incubated with metal salts (0.5-4.0 mM), washed and grown in media with SO 4 2or S 0 . To determine the level of metal ions binding by H 2 S, produced by bacteria, cells were grown in media with metal compounds (0.5-4.0 mM), SO 4 2or S 0 . Biomass was determined by turbidimetric method. In the cultural liquid the content of H 2 S was determined quantitatively by spectrophotometric method, and qualitatively by the presence of metal cations. The content of metal sulfides in the growth medium was determined by weight method. Sulfate and sulfur-reducing bacteria were resistant to 2.0 mM Pb(NO 3 ) 2 , 2.5 mM CuCl 2 , 2.5 mM FeCl 2 × 4H 2 O and 2.0 mM MnCl 2 × 4H 2 O, therefore they are promising for the development of biotechnologies for the purification of water resources contaminated by sulfur and metal compounds. When present in a medium with sulfates or sulfur of 1.0-1.5 mM lead, cuprum (II), iron (II) or manganese (II) ions, they almost completely bind with the H 2 S produced by bacteria in the form of insoluble sulfides, which confirms the negative results of qualitative reactions to their presence in the cultural liquid.

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Section: вступunclassified

Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds

et al. 2018

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“…Biomass accumulation of D. acetoxidans IMV B-7384, its cell size distribution and relative content changes have been carried out [14,15]. Investigated bacterium has been shown to accumulate biomass the most intensively during third-fourth days of its growth.…”

Section: Particularities Of D Acetoxidans Imv B-7384 Growth Physiologymentioning

confidence: 99%

“…Previous research has shown that gram-negative bacterium Desulfuromonas acetoxidans possesses resistance to copper, cadmium, lead, zinc, manganese, iron, etc. [14,15]. Bacteria of Desulfuromonas genus are also shown to be highly effective in term of electricity generation [2,16,17].…”

Section: Introductionmentioning

confidence: 99%

The Anode Biocatalyst with Simultaneous Transition Metals Pollution Control

Bilyy¹,

Vasyliv²,

Hnatush³

2014

Technology and Application of Microbial Fuel Cells

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“…Three-, tetraand deca-heme c-type cytochromes in Shevanella frigidimarina, Desulfovibrio vulgaris, Desulfuromonas acetoxidans are localized between the inner and outer membranes and in the periplasm, through which electrons from the cytoplasm from the reactions of organic compounds oxidation are transferred to the outside of the cells, where the metal ions are reduced (Gescher & Kappler, 2012). Bacteria of the Desulfuromonas genus oxidize organic substrates using metals with variable valence as electron acceptors (Moroz et al, 2014;Moroz et al, 2016), reduce and transform them into non-toxic or less toxic for living organisms forms (Vasyliv et al, 2011;Bilyy et al, 2014;Maslovska et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Influence of potassium dichromate on the reduction of sulfur, nitrate and nitrite ions by bacteria Desulfuromonas sp.

Moroz

Hnatush

Yavorska

et al. 2022

Regul. Mech. Biosyst.

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This article presents the regularities of reduction of sulfur, nitrate and nitrite ions by sulfur reducing bacteria Desulfuromonas sp., which were isolated from the water of the man-made Yavorivske Lake (Lviv Region, Ukraine), under the influence of potassium dichromate. This bacteria in the process of anaerobic respiration can use and reduce different electron acceptors, such as sulfur, nitrates, nitrites, oxidized forms of heavy metals, in particular, hexavalent chromium. Technogenically altered ecotopes are characterized by complex pollution, so several electron acceptors are available to bacteria at the same time. Strains of microorganisms isolated from such ecotopes are adapted to unfavourable conditions and therefore have high biotechnological potential. The purpose of this work was to investigate the regularities of elemental sulfur, nitrate or nitrite ion usage by sulfidogenic bacteria of Desulfuromonas genus in conditions of simultaneous presence in the medium of another electron acceptor – Cr(VI), to establish the succession of reduction of electron acceptors by strains of these bacteria and to evaluate the efficiency of their possible application in technologies of complex purification of the environment from metal compounds and other inorganic toxicants. Bacteria were grown under anaerobic conditions in Kravtsov-Sorokin medium without SO42– and without Mohr’s salt for 10 days. To study the efficiency of sulfur, nitrate or nitrite ions’ reduction at simultaneous presence in the medium of Cr(VI) bacteria were sown in media with elemental sulfur, NaNO3, NaNO2 or K2Cr2O7 to final S0, NO3–, NO2–or Cr(VI) concentration in the medium of 3.47 (concentration of SO42– in medium of standard composition) or 1.74, 3.47, 5.21, 6.94 and 10.41 mM. Biomass was determined by the turbidimetric method, and the concentrations of nitrate, nitrite, ammonium ions, hydrogen sulfide, Cr(VI), Cr(ІІІ) in cultural liquid were determined spectrophotometrically. It has been established that Cr(VI) inhibits the biomass accumulation and hydrogen sulfide production by bacteria of Desulfuromonas sp. after simultaneous addition into the medium of 3.47 mM S0 and 1.74–10.41 mM Cr(VI). In the medium with the same initial content (3.47 mM) of S0 and Cr(VI) bacteria produced Cr(III) at concentrations 3.3–3.4 times higher than that of hydrogen sulfide. It has been shown that K2Cr2O7 inhibits biomass accumulation, nitrate ions’ reduction and ammonium ions’ production by bacteria after simultaneous addition into the medium of 3.47 mM NO3– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO3– and 3.47 mM Cr(VI). In the medium with the same initial content (3.47 mM) of NO3– and Cr(VI) bacteria reduced up to 1.2 times more nitrate ions than Cr(VI) with the production of ammonium ions at concentrations the same times higher than those of Cr(III). It has been established that K2Cr2O7 inhibits biomass accumulation, nitrite ions’ reduction and ammonium ions’ production by bacteria after simultaneous addition into the medium of 3.47 mM NO2– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO2– and 3.47 mM Cr(VI). In the medium with the same initial content of (3.47 mM) NO2– and Cr(VI) the reduction of Cr(VI) by bacteria was only slightly, up to 1.1 times, lower than the reduction of nitrite ions, almost the same concentrations of trivalent chromium and ammonium ions were detected in the cultural liquid. The processes of nitrate and nitride reduction carried out by bacteria of Desulfuromonas genus were revealed to be less sensitive to the negative influence of sodium dichromate, as compared with the process of sulfur reduction, because in the media with the same initial content (3.47 mM) of NO3– or NO2– and Cr(VI) bacteria produced 1.1–1.2 times more NH4+ than Cr(III), but in the medium with the same initial content (3.47 mM) of S0 and Cr(VI) ) bacteria produced over than three times more Cr(III) than hydrogen sulfide. Our data allow us to conclude that bacteria of Desulfuromonas genus, the investigated strains of which are adapted to high concentrations (up to 10.41 mM) of inorganic toxicants, play an important role in the geochemical cycles of sulfur, nitrogen and chromium in aquatic environments that have been under anthropogenic influence.

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The changes of spectroscopic characteristics of sulfurreducing bacteria Desulfuromonas acetoxidans under the influence of different metal ions

Cited by 5 publications

References 3 publications

Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds

Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds

The Anode Biocatalyst with Simultaneous Transition Metals Pollution Control

Influence of potassium dichromate on the reduction of sulfur, nitrate and nitrite ions by bacteria Desulfuromonas sp.

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