New Method for Simultaneous Arsenic and Selenium Speciation Analysis in Seafood and Onion Samples

Abstract

This paper presents a new method for the simultaneous speciation analysis of arsenic (As(III)-arsenite, As(V)-arsenate, DMA-dimethylarsinic acid, MMA-methylarsonic acid, and AsB-arsenobetaine) and selenium (Se(IV)-selenite, Se(VI)-selenate, Se-Methionine, and Se-Cystine), which was applied to a variety of seafood and onion samples. The determination of the forms of arsenic and selenium was undertaken using the High-Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC–ICP–MS) analytical technique. The separation of both organic and inorganic forms of arsenic and selenium was performed using two analytical columns: an anion exchange column, Dionex IonPac AS22, containing an alkanol quaternary ammonium ion, and a double bed cation–anion exchange guard column, Dionex Ion Pac CG5A, containing, as a first layer, fully sulfonated latex for cation exchange and a fully aminated layer for anion exchange as the second layer. The ammonium nitrate, at pH = 9.0, was used as a mobile phase. The method presented here allowed us to separate the As and Se species within 10 min with a suitable resolution. The applicability was presented with different sample matrix types: seafood and onion.

Atomic spectrometry update: review of advances in elemental speciation

“…Two papers cover the simultaneous determination of metal/ metalloids, including Se, in foodstuffs by employing HPLC-ICP-MS. One describes the determination of As and Se in onions and seafood samples. 83 A complete and detailed description on the conditions affecting separation is given aer eight chromatographic columns and various mobile phases were critically evaluated. The separation of both inorganic and organic forms of As and Se was completed using two analytical separation modes: AEC and double bed CEC with a mobile phase of NH 4 NO 3 at pH 9.0 used as mobile phase under gradient elution owing at 1.0 mL min À1 and 0.1% (v/v) MeOH was added to enhance the signal response of As.…”

Atomic Spectrometry Update: review of advances in elemental speciation

“…Two papers cover the simultaneous determination of metal/metalloids, including Se , in foodstuffs by employing HPLC-ICP-MS. One describes the determination of As and Se in onions and seafood samples. 83 A complete and detailed description on the conditions affecting separation is given after eight chromatographic columns and various mobile phases were critically evaluated. The separation of both inorganic and organic forms of As and Se was completed using two analytical separation modes: AEC and double bed CEC with a mobile phase of NH 4 NO 3 at pH 9.0 used as mobile phase under gradient elution flowing at 1.0 mL min −1 and 0.1% (v/v) MeOH was added to enhance the signal response of As.…”

Aptamer and Electrochemical Aptasensor towards Selenate Ions (SeO₄^2-)

“…For the speciation analysis and determination of anionic selenium forms in water so far, many spectroscopic techniques have been developed, including inductively coupled plasma-massspectrometry (ICP-MS) [6][7][8], inductively coupled plasma-optical emission spectroscopy (ICP-OES) [9], hydride generation atomic absorption spectrometry (HGAAS) [10,11], nuclear magnetic resonance Imaging (NMRI) [12], neutron activation analysis [13] or fluorometry [14]. Also, separation techniques such as high-performance liquid chromatography (HPLC) [15], solid-phase extraction (SPE) [16], ion-exchange chromatography (IEC) [17] or capillary electrophoresis [18] have been employed to improve the performance of the existing selenium detection methods. Unfortunately, a laboratory equipped with expensive analytical devices is indispensable for carrying out such analyses Disclaimer/Publisher's Note: The statements, opinions, and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s).…”