2014
DOI: 10.1146/annurev-virology-031413-085437
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New Methods in Tissue Engineering: Improved Models for Viral Infection

Abstract: New insights in the study of virus and host biology in the context of viral infection are made possible by the development of model systems that faithfully recapitulate the in vivo viral life cycle. Standard tissue culture models lack critical emergent properties driven by cellular organization and in vivo–like function, whereas animal models suffer from limited susceptibility to relevant human viruses and make it difficult to perform detailed molecular manipulation and analysis. Tissue engineering techniques … Show more

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Cited by 23 publications
(12 citation statements)
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References 215 publications
(243 reference statements)
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“…Moreover, because the human hepatitis virus cannot infect mice or rats, a rodent model of virus induced hepatitis is currently unavailable. Furthermore, the cell culture models of virus infection, including infection by hepatotropic viruses, cannot be satisfactorily manipulated [ 20 ]. Because both in vitro and in vivo models of liver cancer pathogenesis are limited by their restricted host range and hepatotropism, LPS-induced inflammation is an acceptable alternative for investigating the mechanisms of inflammation-induced hepatopathogenesis [ 21 ].…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, because the human hepatitis virus cannot infect mice or rats, a rodent model of virus induced hepatitis is currently unavailable. Furthermore, the cell culture models of virus infection, including infection by hepatotropic viruses, cannot be satisfactorily manipulated [ 20 ]. Because both in vitro and in vivo models of liver cancer pathogenesis are limited by their restricted host range and hepatotropism, LPS-induced inflammation is an acceptable alternative for investigating the mechanisms of inflammation-induced hepatopathogenesis [ 21 ].…”
Section: Discussionmentioning
confidence: 99%
“…However, the majority of hepatoma cell lines do not elicit a strong immune response and IFNL expression. Novel model systems including stem cell derived hepatocytes [106][107][108] and tissue engineering systems [109] might in the future allow to more faithfully mimic host responses to hepatotropic virus infection.…”
Section: Ifnl In In Vitro Models Of Hepatitis C In Line With Observamentioning
confidence: 99%
“…While this can certainly also be seen as an advantage by allowing the study of epithelial interactions without possible confounding effects from other cell types, it can also be seen as a limitation. Co-cultures and tissue engineering approaches will generate more complex stem cell-derived tissues which will allow the study of interactions with immune cells or other cell types (reviewed in Ramanan et al, 2014). For example, recently an exciting 3D model of human colon has been published, repopulating decellularized colonic mucosa with modified organoidderived epithelial cells, endothelial cells and myofibroblasts (Chen et al, 2016).…”
Section: Anaerobic Bacteriamentioning
confidence: 99%