New micro-turbidimetric method for determination of protein in cerebrospinal fluid and urine.

Iwata, Jihei; Nishikaze, O

doi:10.1093/clinchem/25.7.1317

Cited by 105 publications

(18 citation statements)

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“…In clinical laboratories, turbidimetric methods such as the benzethonium chloride method (12), and dyebinding methods utilizing pigments such as CBB and PRM (2,3) are commonly used for measurement of urinary total protein. Because pigment methods have a higher sensitivity than turbidimetric methods (13), and the AV6B method is a pigment method, we compared the AV6B method with two other pigment methods, the CBB and PRM methods.…”

Section: Discussionmentioning

confidence: 99%

Development of a new method for measuring total urinary protein using acid violet 6B pigment

Iijima¹,

Cho²,

Sakai³

et al. 2003

Clinical Laboratory Analysis

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We have developed a new method for measuring total urinary protein using acid violet 6B (AV6B) pigment. Nine purified components of human urinary proteins and urine samples collected randomly from 123 diabetic outpatients were used. There were 62, 36, and 25 cases of prenephropathy, early nephropathy, and overt nephropathy, respectively. All samples were measured by Coomassie brilliant blue G 250 (CBB), pyrogallol red-molybdate (PRM), and AV6B methods using an optical photometer. In healthy subjects, the major components of urinary proteins, such as gamma-globulins, IgG, IgA Tamm-Horsfall protein, and transferrin, the reactivity values of the AV6B and PRM methods were similar. The CBB method was the least sensitive of the three methods. In the urine samples from diabetic patients, the urinary protein values measured by the AV6B method were higher than those measured by the CBB method in the prenephropathy stage. The values obtained by the AV6B method (y) correlated well with those from the CBB method (x) (y=1.243x+3.61, r=0.904). When the values from the AV6B method (y) were compared to those from the PRM method (x), correlation was low (y=1.406x-29.15, r=0.786). In conclusion, the AV6B method was more useful than the CBB and PRM methods for low levels of urinary protein.

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Section: Discussionmentioning

confidence: 99%

Development of a new method for measuring total urinary protein using acid violet 6B pigment

Iijima¹,

Cho²,

Sakai³

et al. 2003

Clinical Laboratory Analysis

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“…* and in a limited 3-week study in mice and rats by Suurmeijer et u I .~ However, except for the monitoring of circulating serum iron level^,^ no metabolic investigations were performed in these studies and no examination was made of the possibility of endo- Several investigators have demonstrated that a high blood level of tracer is required to saturate the systemic mononuclear phagocytic system before significant uptake by the mesangium is observed. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]22 The large intravenous dose of Imposil required to produce significant mesangial uptake in this study produced a toxic reaction which may have been associated with a redistribution of renal blood flow, resulting in disappearance of tracer from some glomerular capillaries whilst it was still visible in vasa rectae (Fig. I).…”

Section: Discussionmentioning

confidence: 99%

Persistence of inert macromolecules (imposil) in the rat mesangium and glomerular functional disturbance

Goode

Davison

Gowland

et al. 1984

The Journal of Pathology

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Imposil iron-dextran is an inert tracer that has been used to study mesangial uptake and clearance of macromolecular material from the glomerular circulation. Such a tracer may be a useful marker of altered mesangial function in animals with some forms of glomerulonephritis. We have studied mesangial handling of intravenously injected Imposil (50 mg/100 g body weight) in normal rats by light, immunofluorescence and electron microscopy for up to 3 months. Mesangial cell uptake was maximal at 48-54 h. Extrusion and drainage of tracer to the vascular pole and distal tubule was evident at 3 days but iron was still present in mesangial cells at 3 months. Possible functional renal impairment resulting from persistent mesangially sequestered tracer was examined by measuring daily urine protein and iron excretion. A possible relationship between failure of mesangial cells to eliminate inert tracer and increasing glomerular permeability is demonstrated, suggesting that Imposil and similar inert macromolecules cannot be used for long-term studies of mesangial function.

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“…Within 30 minutes of adding blood to the urine samples, the urine protein and creatinine concentrations were assessed using a benzethonium chloride assay (Urine/CSF protein method, Roche Diagnostics) (Iwata and Nishikaze 1979;Luxton et al 1989), and the Jaffe method (Creatinine Method, Roche Diagnostics) ( Bartels et al 1972), respectively.…”

Section: Methodsmentioning

confidence: 99%

Does blood contamination of urine compromise interpretation of the urine protein to creatinine ratio in dogs?

Jillings

Squires

Azarpeykan

et al. 2019

New Zealand Veterinary Journal

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AIMS: To determine the effect of contamination of urine with 0-5% blood, varying in haematocrit and protein concentrations, on the urine protein to creatinine ratio (UPC) in dogs, and to determine whether the colour of urine can be used to aid interpretation of UPC results. METHODS: Urine samples were collected by free catch from 18 dogs, all of which had UPC <0.2. Venous blood samples were also collected from each dog, and the blood from each dog was added to its own urine to produce serial concentrations of 0.125-5% blood. The colour of each urine sample was recorded by two observers scoring them as either yellow, peach, orange, orange/red or red. Protein and creatinine concentrations were determined, and dipstick analysis and sediment examination was carried out on each sample. Based on colour and dipstick analysis, samples were categorised as either having microscopic, macroscopic or gross haematuria. A linear mixed model was used to examine the effect of blood contamination on UPC. RESULTS: The uncontaminated urine of all 18 dogs had a UPC <0.2. Adding blood to the urine samples resulted in an increase in UPC at all contamination concentrations compared to the noncontaminated urine (p<0.001). None of the 54 samples with microscopic haematuria had UPC >0.5. For 108 samples with macroscopic haematuria the UPC was >0.5 in 21 samples (19.4 (95% CI=13.1-27.9)%), and for 54 samples with gross haematuria 39 (72 (CI=59.1-82.4)%) had a UPC >0.5. No samples had a UPC >2.0 unless the blood contamination was 5% and only 3/18 (17%) samples at this blood contamination concentration had a UPC >2.0.

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New micro-turbidimetric method for determination of protein in cerebrospinal fluid and urine.

Cited by 105 publications

References 0 publications

Development of a new method for measuring total urinary protein using acid violet 6B pigment

Development of a new method for measuring total urinary protein using acid violet 6B pigment

Persistence of inert macromolecules (imposil) in the rat mesangium and glomerular functional disturbance

Does blood contamination of urine compromise interpretation of the urine protein to creatinine ratio in dogs?

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