New Micropeptins with Anti-Neuroinflammatory Activity Isolated from a Cyanobacterial Bloom

Kirk, Riley D.; He, Haiyin; Wahome, Paul G.; Wu, Shi‐Biao; Carter, Guy T.; Bertin, Matthew J.

doi:10.1021/acsomega.1c02025

Cited by 5 publications

(8 citation statements)

References 29 publications

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“…The material in this library was derived from a cyanobacterial bloom in Muskegon, MI . This library was used by our laboratory previously to isolate new antineuroinflammatory micropeptins . In addition, to these antineuroinflammatories there were also chromatography fractions that showed strong cytotoxicity to murine BV-2 microglial cells .…”

Section: Methodsmentioning

confidence: 99%

“…8 This library was used by our laboratory previously to isolate new antineuroinflammatory micropeptins. 31 In addition, to these antineuroin-flammatories there were also chromatography fractions that showed strong cytotoxicity to murine BV-2 microglial cells. 31 We purchased preparative amounts of these fractions (ca.…”

Section: New Toxin Isolationmentioning

confidence: 99%

“…31 In addition, to these antineuroin-flammatories there were also chromatography fractions that showed strong cytotoxicity to murine BV-2 microglial cells. 31 We purchased preparative amounts of these fractions (ca. 3− 15 mg/fraction) with the intent of isolating new cyanotoxins.…”

Section: New Toxin Isolationmentioning

confidence: 99%

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Temporal Dynamics of Cyanobacterial Bloom Community Composition and Toxin Production from Urban Lakes

Maurer,

Xia,

Kim

et al. 2024

ACS EST Water

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With a long evolutionary history and a need to adapt to a changing environment, cyanobacteria in freshwater systems use specialized metabolites for communication, defense, and physiological processes. Furthermore, many cyanobacterial specialized metabolites and toxins present significant human health concerns due to their liver toxicity and their potential impact to drinking water. Gaps in knowledge exist with respect to changes in species diversity and toxin production during a cyanobacterial bloom (cyanoHAB) event; addressing these gaps will improve understanding of impacts to public and ecological health. In the current report we detail community and toxin composition dynamics during a late bloom period. Species diversity decreased at all study sites over the course of the bloom event, and toxin production reached a maximum at the midpoint of the event. We also isolated three new microcystins from a Microcystis dominated bloom (1−3), two of which contained unusual doubly homologated tyrosine residues (1 and 2). This work provokes intriguing questions with respect to the use of allelopathy by organisms in these systems and the presence of emerging toxic compounds that can impact public health.

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Section: Methodsmentioning

confidence: 99%

Section: New Toxin Isolationmentioning

confidence: 99%

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Temporal Dynamics of Cyanobacterial Bloom Community Composition and Toxin Production from Urban Lakes

Maurer,

Xia,

Kim

et al. 2024

ACS EST Water

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“…Finally, photosynthetic prokaryotes are still largely unexplored biomanufacturing platforms, despite producing a vast repertoire of unique compounds [338], including molecules with antioxidant and anti-inflammatory properties [339,340] like the pigment-protein complex phycocyanin, which is a selective inhibitor of pro-inflammatory oxylipin synthesis [341,342], and various polysaccharides [343][344][345] and peptides [346][347][348]. Last but not least, an increasing number of genetic engineering and synthetic biology tools [349] can be employed to produce recombinant therapeutics in cyanoprokaryotes [350], as already reported for immunomodulatory proteins [351].…”

Section: A Road Map For Clinical Uses Of Microalgae In Chronic Inflam...mentioning

confidence: 99%

The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics

Cutolo,

Caferri,

Campitiello

et al. 2023

Marine Drugs

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Rheumatoid arthritis (RA) is an invalidating chronic autoimmune disorder characterized by joint inflammation and progressive bone damage. Dietary intervention is an important component in the treatment of RA to mitigate oxidative stress, a major pathogenic driver of the disease. Alongside traditional sources of antioxidants, microalgae—a diverse group of photosynthetic prokaryotes and eukaryotes—are emerging as anti-inflammatory and immunomodulatory food supplements. Several species accumulate therapeutic metabolites—mainly lipids and pigments—which interfere in the pro-inflammatory pathways involved in RA and other chronic inflammatory conditions. The advancement of the clinical uses of microalgae requires the continuous exploration of phytoplankton biodiversity and chemodiversity, followed by the domestication of wild strains into reliable producers of said metabolites. In addition, the tractability of microalgal genomes offers unprecedented possibilities to establish photosynthetic microbes as light-driven biofactories of heterologous immunotherapeutics. Here, we review the evidence-based anti-inflammatory mechanisms of microalgal metabolites and provide a detailed coverage of the genetic engineering strategies to enhance the yields of endogenous compounds and to develop innovative bioproducts.

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“…Tandem mass spectrometry (LC-MS/MS) was performed using a ThermoFisher Scientific LTQ XL linear ion trap mass spectrometer with an electrospray ionization (ESI) source. Analytical HPLC was carried out using a Dionex UltiMate 3000 HPLC system equipped with a micro vacuum degasser, an autosampler, and a diode-array detector [25]. Samples were separated on a Phenomenex Luna C 18 column (5 µm; 150 × 4.6 mm).…”

Section: Lc-ms/ms Analysis and Molecular Networkingmentioning

confidence: 99%

What Is Authentic Maple Water? A Twelve-Month Shelf-Life Study of the Chemical Composition of Maple Water and Its Biological Activities

Torrey

Liu

et al. 2023

Foods

Self Cite

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Maple water (maple sap) products are produced from sap tapped directly from maple trees, but there is confusion and lack of industry consensus and consumer knowledge as to what constitutes ‘authentic’ maple water. With an immense potential for growth in the multi-billion dollar functional beverage market, the market promotion of maple water products hinges on establishing standards of identity (SI), which are currently lacking. Herein, we aim to provide publishable SI and compositional chemistry findings of maple water. The chemical composition (including polyphenols, sugars, amino acids, and organic acids) of a pasteurized maple water was monitored over a 12-month (at 0, 4, 8, and 12 months) shelf-life. Furthermore, LC-MS/MS and molecular networking-based methods were developed to identify the phytochemical profile of a maple water extract (MWX) and to compare it to a previously chemically characterized phenolic-enriched maple syrup extract (MSX). Both MSX and MWX have similar phytochemical profiles and chemical characteristics. In addition, MSX and MWX showed moderate antioxidant capacity (in free radical scavenging and anti-tyrosinase assays) and anti-inflammatory effects (in soluble epoxide hydrolase and cyclooxygenase-2 inhibition assays). Our findings provide critical information on the SI and stability (in chemical composition) of maple water, which will help define, authenticate, and distinguish it from other functional beverages, thereby positioning the maple industry for promotion and growth in this market sector.

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New Micropeptins with Anti-Neuroinflammatory Activity Isolated from a Cyanobacterial Bloom

Cited by 5 publications

References 29 publications

Temporal Dynamics of Cyanobacterial Bloom Community Composition and Toxin Production from Urban Lakes

Temporal Dynamics of Cyanobacterial Bloom Community Composition and Toxin Production from Urban Lakes

The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics

What Is Authentic Maple Water? A Twelve-Month Shelf-Life Study of the Chemical Composition of Maple Water and Its Biological Activities

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