NEW miniSTR loci D10S1248, D14S1434, D22S1045, D4S2364, D2S441, D1S1677 validation and optimization on blood samples

Ünsal, Tuğba; Filoğlu, Gönül; Sipahi, Elif; Altuncul, H.; Rayimoğlu, Gülten

doi:10.1016/j.fsigss.2011.09.098

Cited by 1 publication

(2 citation statements)

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“…Due to this reason, fabrics such as nylon and cotton, which are mostly encountered at the scene, were chosen in this study. It is known that in washed blood-stained nylon or cotton fabric samples, a genotyping problem can be experienced with classical STR loci, which occupy more space on DNA, and so generally no results can be obtained (Unsal et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, by the help of developing technology, DNA profiles which allow individualization serve as a significant evidence to investigate criminal cases. Due to this reason, analyses of biological materials which are obtained from the crime scene are extremely important and it is possible to carry these analyses to better points (Bond and Hammond 2008;Kamphausen et al 2015;Unsal et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Human identification from washed blood stains

2021

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Background Among the physical evidence, bloodstain is one of the most common biological pieces of evidence at the crime scene, especially in violent crimes. Bloodstains are frequently seen at the crime scene and can be easily detected due to their color and structure. Because blood and bloodstains can potentially be evidence of a crime, offenders often tend to clean or wash them to get rid of relevant evidence. Some researchers think that washed bloodstains cannot obtain enough quality DNA for DNA profiling. However, some studies have shown that bloodstains on a piece of fabric can be used for DNA profiling even after washing. This study, it is aimed to determine whether a sufficient amount of DNA can be obtained for DNA profiling used for forensic purposes from blood-stained nylon and cotton fabrics washed at different temperatures such as 40 °C, 60 °C and 90 °C. Results In this study, bloodstains were created on cotton and nylon fabrics in a representative crime scene. After washing with laundry detergent at 40 °C, 60 °C and 90 °C was performed, studies were conducted in order to make bloodstains visible and obtain DNA for genetic profiling. The result is that more DNA is lost due to easy exposure to external factors in the non-absorbent nylon fabric type compared to the absorbent cotton fabric. Moreover, the rates of obtaining DNA from bloodstains on different types of fabrics washed at several temperatures are shown in this study. Conclusions It was determined that while a sufficient amount of DNA for the forensic genetic profiling can be obtained from cotton and nylon fabrics washed at 40 and 60 °C, a sufficient amount could not be obtained from the ones washed at 90 °C. And that shows even if bloodstained fabrics are washed at 60 °C, the fabrics still can be considered as physical evidence of the crime and can be associated with the scene, perpetrator and victim triangle.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%