Spinach is an important winter vegetable and it is produced in all regions in Turkey. There are many diseases affecting the yield and quality in its production. Viruses have an important role among them. One of these virus diseases is Beet necrotic yellow vein virus (BNYVV), called Rhizomania disease. The presence of BNYVV in the spinach plant was determined by serological methods in Turkey, but no studies were conducted at the molecular level. For this purpose, field studies were carried out in 2017-2018 in order to determine the presence of BNYVV in the fields of spinach cultivation at the level of Çanakkale province and its districts. In these field studies, 66 samples were collected from spinach plants showing typical virus symptoms. The presence of BNYVV in the samples was determined by DAS-ELISA test, a method based on virus protein. According to the DAS-ELISA test, 12 out of 66 samples were infected with BNYVV. One of the infected samples was selected (CNKSpinach39) and the coat protein gene (CP) was amplified by RT-PCR. Its RT-PCR product was purified and sequenced. The amino acid (aa) sequences of this isolate was deduced based on its nt sequence. These nt and aa sequences were compared with the related sequences of the world BNYVV isolates and their similarities and phylogenetic relationships were determined. As a result of multiple sequence comparisons based on nt and aa, it was determined that CNKSpinach39 isolates showed similarities with 95-99% of the world isolates. As a result of the phylogenetic analysis, it was determined that the isolate CNKSpinach39 was found in the B pathotype. In this study, the first BNYVV B pathotype was found in spinach in our country.