Effect of the burial depth and environmental factors on the seasonal germination of bearded sprangletop (Leptochloa fusca [L.] Kunth ssp. fascicularis [Lam.] N. Snow)Bearded sprangletop recently was introduced to Turkey, where it has adapted to rice cultivation and become an important weed in many regions of the country. Experiments were conducted to determine the effects of light, temperature, osmotic stress, salt stress, pH and flooding on bearded sprangletop germination and emergence. Mature bearded sprangletop seeds were collected from rice fields in August 2008. Approximately 1250 seeds were wrapped in plastic fabric and buried at 2 or 10 cm in pots in flooded or non-flooded conditions. In this experiment, seed germination in the light from the flooded and non-flooded treatments began in the spring, peaked in summer and decreased in the fall. This pattern was repeated the following year after exposing the seeds to natural seasonal temperature changes. As either the level of water stress or NaCl concentration increased, cumulative seed germination decreased.No seed germinated when the NaCl concentration exceeded 400 mole. The level of seedling emergence decreased with an increasing burial depth. Under the flooded conditions, emergence began in late April and continued until early July, with peak emergence in the first week of June. Under the non-flooded conditions, bearded sprangletop emerged later than under the flooded conditions and the overall level of emergence was lower. The depth of burial and water stress appear to be the most important factors that limit seed germination. Germination was stimulated by light, suggesting that the seeds are positively photoblastic.
Echinochloa crus-galli (L.) P. Beauv. (barnyardgrass) is an annual weed that is native to Asia and found throughout the world. The broad ecological tolerance and competitive ability of E. crus-galli makes it the most important weed species in rice. Genetic studies of plants are becoming increasingly common because reliable information is necessary to better understand population dynamics, occurrence of herbicide resistance, and demographic data. Echinochloa crus-galli populations from 34 different locations in Turkey were compared with respect to morphological differences and genetic variation. For morphological variation, five seeds of each population were sown in pots and grown in a screenhouse using a randomized block design. Morphological parameters such as germination speed, flowering time, leaf area, plant height, spikelet length, above-ground biomass, root dry weight and number of seeds were measured. Distinct differences among populations with respect to hierarchical cluster analysis were observed. Genetic variations among populations were performed using random amplified polymorphic DNA (RAPD) markers. The seven RAPD primers amplified 55 bands whose molecular weight varied between 200 and 4000 bp. The percentage of polymorphic bands was 74.54%. Results showed high morphological and genetic variability among individual genotypes within geographic locations. Phenotypic and genetic variability among E. crus-galli populations would be influenced by agricultural practices, crop characteristics, geographic location and herbicide pressure. Differences between weed populations may affect response to chemical or biological control.
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