New observations on β-glucuronidase in human cervical cancer

CHARACTERIZATION OF TWO HELA SUBLINES: TCRC-1 PRODUCES REGAN ISOENZYME AND TCRC-2, NON-REGAN ISOENZYME

“…With regard to the enrichment of TCRC-1 with ß-glucuronidase, current studies are directed to evaluating the significance of this finding in relation to expression of Regan isoenzyme and to its well-known existence in neoplastic tissue (5,18) .…”

Lysosomes in cancer cells

“…There is also evidence that invasiveness of tumours shows a relationship to their content of lysosomal enzymes. Increased invasiveness and a high mitotic index of tumours of the female genital tract is correlated with levels of cathepsin Bi (Blackwood, Mandl, and Long, 1965) and beta-glucuronidase (Watts and Goldberg, 1969). Transplantable tumours grown subcutaneously showed higher activities of beta-N-acetylglucosaminidase and beta-glucuronidase than when grown intraperitoneally in a mainly non-invasive form.…”

“…As recently reviewed by Poole (1973), several studies have revealed higher specific activities of lysosomal enzymes in solid tumours than in their tissues of origin. These include beta-glucuronidase in tumours of the mouse (Karunairatnam, Kerr, and Levvy, 1949;Cohen and Bittner, 1951) and humans (Fishman and Anlyan, 1947;Boyland, Wallace, and Williams, 1955;Watts and Goldberg, 1969), and beta-N-acetylglucosaminidase (Conchie and Levvy, 1957) and arylsulphatases in human tumours (Boyland et al, 1955;Dzialoszynski, Frohlich, and Kroll, 1966). Shamberger and Rudolph (1967) have found increased acid phosphatase, cathepsin D, and betaglucuronidase in mouse skin cancers as compared with normal skin.…”

Lysosomes in cancer cells

“…Increased invasiveness and a high mitotic index of tumours of the female genital tract is correlated with levels of cathepsin Bi (Blackwood, Mandl, and Long, 1965) and beta-glucuronidase (Watts and Goldberg, 1969). Transplantable tumours grown subcutaneously showed higher activities of beta-N-acetylglucosaminidase and beta-glucuronidase than when grown intraperitoneally in a mainly non-invasive form.…”

“…These include beta-glucuronidase in tumours of the mouse (Karunairatnam, Kerr, and Levvy, 1949;Cohen and Bittner, 1951) and humans (Fishman and Anlyan, 1947;Boyland, Wallace, and Williams, 1955;Watts and Goldberg, 1969), and beta-N-acetylglucosaminidase (Conchie and Levvy, 1957) and arylsulphatases in human tumours (Boyland et al, 1955;Dzialoszynski, Frohlich, and Kroll, 1966). Shamberger and Rudolph (1967) have found increased acid phosphatase, cathepsin D, and betaglucuronidase in mouse skin cancers as compared with normal skin.…”