New or little-known species of Chaetocladius s. str. Kieffer, 1911 (Diptera: <br />Chironomidae: Orthocladiinae) from the Amur River basin (Russian Far East)

Abstract

Chironomids of the subgenus Chaetocladius s. str. from the Amur River basin are revised using both morphological characters and molecular data. Three new species, C. egorych sp. nov., C. lopatinskiy sp. nov. and C. yavorskayae sp. nov., are described and figured. The pupa of C. fedotkin is described for the first time. Adult males of C. ligni and C. piger, little-known in the Far East, are redescribed and annotated, and key to males of the Chaetocladius s. str. from the Amur River basin is provided. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. Comparisons with corresponding regions of COI between 5 species in the subgenus produced K2P genetic distances of 8.3-12.6%, values well associated with interspecific variation. Molecular data were also used for the reconstruction of the phylogenetic relationships within the subgenus Chaetocladius s. str.

On the genus Chaetocladius s. str. Kieffer, 1911 from Switzerland with descriptions of five new relic species occurring in glacial alpine springs and streams (Diptera, Chironomidae)

“…Although some resemblance can be found between the five new described species and other related members of the Chaetocladius genus, the taxonomic position cannot be clarified until current comprehensive work on the genus is complete. Worldwide there are actually about 79 known Chaetocladius species, which include the hitherto listed 69 species by Ashe and O'Connor (2012) and the recently described 10 species by: Makarchenko et al (2017), Moubayed-Breil (2017), Moubayed-Breil and Dia (2017), Rossaro et al (2017). Consequently, the updated previous list of 79 species is upgraded to 84, which currently include the five new described species in this paper.…”

“…Based on knowledge provided on the taxonomy, geographical distribution and ecology of the known Chaetocladius species from Europe and the Palaearctic Region (Brundin 1947(Brundin , 1956Pankratova 1970;Saether 1980Saether , 1990Caspers 1987, Cranston et al 1989, Bhattacharyay et al 1993, Lods-Crozet 1998, Moubayed 1989, Makarchenko and Makarchenko 2001, 2004, 2007, 2011a-b, Langton and Pinder 2007, Lencioni et al 2007, Zelentsov 2007, Stur and Spies 2011, Ashe and O'Connor 2012, Kobayashi 2012, Wang et al 2012, Saether and Spies 2013, Makarchenko et al 2017, Moubayed-Breil 2017, Rossaro et al 2017, the genus Chaetocladius Kieffer, 1911 currently comprises worldwide about 79 valid species.…”

Chaetocladius berythensis sp. n., C. callauensis sp. n., C. guardiolei sp. n. and C. parerai sp. n., four relict species inhabiting glacial springs and streams in eastern Pyrenees and Lebanon (Diptera: Chironomidae)

“…Knowledge on the taxonomy, geographical distribution and ecology of the known Chaetocladius s. str. species from Europe and neighbouring areas are included in Goetghebuer (1940Goetghebuer ( -1950, Brundin (1947Brundin ( , 1956, Pankratova (1970), Caspers (1987), Cranston et al (1989), Moubayed (1989), Saether (1990), Langton (1991), Makarchenko & Makarchenko (2001, 2003, 2004, 2006a-b, 2007, 2009, 2011a-b, 2013a-b, 2018, Langton & Pinder (2007), Zelentsov (2007), Langton & Armitage (2010), Stur & Spies (2011), Ashe & O'Connor (2012), Kobayashi (2012); Wang et al (2012); Saether & Spies (2013), Makarchenko et al (2014), Moubayed-Breil (2017), Moubayed-Breil & Dia (2017), Rossaro et al (2017), Moubayed-Breil & Lods-Crozet (2017), , Moubayed-Breil & Bitusik (2019). Worldwide, the genus Chaetocladius Kieffer, 1911 comprises about 75 species of which, only about 42 are reported from Europe.…”

Morphological description and DNA barcoding of Chaetocladius (Chaetocladius) elisabethae sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Moscow Region

“…Twenty five species of Chaetocladius s. str. are known from Russia (Zelentsov & Shilova 1996;Zelentsov 2007;Ashe & O'Connor 2012;Makarchenko et al 2017a), of which 17 species were described as new from the Russian Far East and bordering territories (Makarchenko & Makarchenko 2001, 2004, 2006a-b, 2009, 2011a-b, 2012, 2017Makarchenko et al 2017a).…”

“…nov. from the Moscow Region are provided in comparison with closely related species of Chaetocladius s. str. The DNA barcode corresponding to the 650-bp fragment of the mitochondrial gene cytochrome c oxidase I (COI) has been identified as the core of a global bioidentification system at the species level (Hebert et al 2003) and has proved to be useful in the subfamily Orthocladiinae (Silva & Wiedenbrug 2014, Makarchenko et al 2015, 2017a, 2017b.…”