New oxandrolone derivatives by biotransformation using Rhizopus stolonifer

Efficient hydroxylation of functionalized steroids by Colletotrichum lini ST-1

“…5, using 15␣-hydroxy-androst-4-en-3,17-dione (8) or 15␣-hydroxy-16␣,17␣-epoxyprogesterone (13) as substrate, resulted in the accumulation of 11␣,15␣-dihydroxy-androst-4-en-3,17-dione (9) or 11␣,15␣-dihydroxy-16␣,17␣-epoxyprogesterone (14) with extended transformation time. After incubated with C. lini ST-1 for 48 h, the substrates of (8) and (13) could be converted almost completely to (9) and (14). From another perspective, these results indicated that 11␣,15␣-dihydroxy-steroids were formed from steroidal compounds following the subsequent introduction of hydroxyl groups to the 15␣-position and then to the 11␣-position of the steroid molecule.…”

Biotransformation of dehydroepiandrosterone with Macrophomina phaseolina and β-glucuronidase inhibitory activity of transformed products

“…β-Glucuronidase inhibitory activity was evaluated by a biochemical assay, based on the measurement of the absorbance of p-nitrophenol at 405 nm, produced from the enzymatic hydrolysis of chromogenic substrate 36,41 . The reaction mixture, consisting of 5 µL of test compound solution, 185 µL of 0.1 M acetate buffer (pH 7.0) and 10 µL β-glucuronidase solution, was kept for 30 min at 37°C.…”

“…In continuation of our work on biotransformation of steroidal drugs [33][34][35][36] , we screened DHEA (1) against several enzymes, and it was found to be active against β-glucuronidase. We therefore carried out the biotransformation of 1 to produce its transformed products.…”

Cunninghamella blakesleeana-mediated biotransformation of a contraceptive drug, desogestrel, and anti-MDR-Staphylococcus aureus activity of its metabolites

“…In continuation of our work on fungal-mediated synthesis of bioactive steroids [17][18][19][20], including 17a-ethynyl substituted steroidal drugs [21][22][23], an oral contraceptive drug, desogestrel…”