New perspectives on the evolutionary history of hepatitis B virus genotype F

Torres, Carolina; Leone, Flavia Guadalupe Piñeiro y; Pezzano, Silvana Claudia; Mbayed, Viviana Andrea; Campos, Rodolfo Héctor

doi:10.1016/j.ympev.2011.01.010

Cited by 40 publications

(46 citation statements)

References 48 publications

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“…This gt has been found in Native American populations of Alaska, Central and South America (Arauz-Ruiz et al, 1997;Devesa et al, 2008;Livingston et al, 2007b). In Argentina, the Northern region presents the highest prevalence of the country, mainly by the presence of sgt\ \F1b and F4, related to a major Native American ethnical background in this region (Piñeiro y Leone et al, 2008;Torres et al, 2011). Their circulation has been previously reported in the Province of Misiones, both in blood donor population and Native American communities, although the low number of sequences obtained makes the prevalence analysis difficult (Delfino et al, 2014b(Delfino et al, , 2012.…”

Section: Discussionmentioning

confidence: 78%

Molecular epidemiology of hepatitis B virus in Misiones, Argentina

Mojsiejczuk

Torres

Sevic

et al. 2016

Infection, Genetics and Evolution

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Section: Discussionmentioning

confidence: 78%

Molecular epidemiology of hepatitis B virus in Misiones, Argentina

Mojsiejczuk

Torres

Sevic

et al. 2016

Infection, Genetics and Evolution

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“…The full-length genome of Panamanian sequences clustering in particular clades of genotype F, were amplified using two protocols previously published [15], [16]. When a sample failed to yield a good quality product with the one-step protocol, the nested approach was used.…”

Section: Methodsmentioning

confidence: 99%

“…Amino acid substitutions in polymerase, PreS2-S, HBsAg and X-gene were determined by translating the Panamanian nucleotide sequences and comparing them against reference sub-genotype F amino acid sequences [16], [23], using MEGA 5.2 [21].…”

Section: Methodsmentioning

confidence: 99%

“…Full-length genome and partial polymerase (nt 177 to 990) gene datasets were constructed including published reference sequences for sub-genotypes of genotype F [16]. An uncorrelated lognormal relaxed clock (UCLN) [24] was used to evaluate the time of the most recent common ancestor (tMRCA) of each node.…”

Section: Methodsmentioning

confidence: 99%

“…An uncorrelated lognormal relaxed clock (UCLN) [24] was used to evaluate the time of the most recent common ancestor (tMRCA) of each node. Sample collection date was used as a calibration to calculate the substitution rates; additionally, a fixed medium rate of 1.5×10 −5 [16] was used to calculate the tMRCA of each node. The best-fit demographic tree model was selected calculating the path sampling (PS) and stepping stone sampling methods [25].…”

Section: Methodsmentioning

confidence: 99%

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High Diversity of Hepatitis B Virus Genotypes in Panamanian Blood Donors: A Molecular Analysis of New Variants

et al. 2014

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Hepatitis B Virus (HBV) is an infectious agent that causes more than half of the cases of liver disease and cancer in the world. Globally there are around 250 million people chronically infected with this virus. Despite 16% of the cases of liver disease in Central America are caused by HBV, the information regarding its genetic diversity, genotypes and circulation is scarce. The purpose of this study was to evaluate the genetic variability of the HBV genotypes from HBV-DNA positive samples obtained from screening blood donors at the Social Security System of Panama and to estimate its possible origin. From 59,696 blood donors tested for HBV infection during 2010–2012, there were 74 HBV-DNA positive subjects. Analysis of the partial PreS2-S region of 27 sequences shows that 21% of the infections were caused by genotype A, 3% by genotype D and 76% by genotype F. In addition, we were able to confirm circulation of six sub-genotypes A1, A2, A3, D4, F3, F1 and a proposed new sub-genotype denominated F5pan. We found a confinement of sub-genotypes F1 and F5pan to the western area of Panama. The tMRCA analysis suggests a simultaneous circulation of previously described sub-genotypes rather than recent introductions of the Panamanian sub-genotypes in the country. Moreover, these results highlight the need of more intensive research of the HBV strains circulating in the region at the molecular level. In conclusion, Panama has a high HBV genotype diversity that includes a new proposed sub-genotype, an elevated number of PreCore-Core mutations, and confinement of these variants in a specific geographical location.

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