New phosphorylated derivatives of carboxymethylcellulose with osteogenic activity

Leone, Gemma; Torricelli, Paola; Giardino, Roberto; Barbucci, Rolando

doi:10.1002/pat.1041

Cited by 41 publications

(41 citation statements)

References 25 publications

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“…Sodium trimetaphosphate (STMP) is a chemical phosphorylation agent for food proteins [19], carboxymethylcelluose [20] and type I collagen [21]. However, when this simple cyclic polyphosphate molecule is used as a templating analogue without a sequestration agent to stabilise ACP in the form of nanoprecursors, either large extrafibrillar mineral spheres are produced in the vicinity of the collagen fibrils [22] or extrafibrillar apatite crystals are precipitated in a non-oriented manner along the fibrillar surface [23].…”

Section: Introductionmentioning

confidence: 99%

Hierarchical and non-hierarchical mineralisation of collagen

et al. 2011

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Biomineralisation of collagen involves functional motifs incorporated in extracellular matrix protein molecules to accomplish the objectives of stabilising amorphous calcium phosphate into nanoprecursors and directing the nucleation and growth of apatite within collagen fibrils. Here we report the use of small inorganic polyphosphate molecules to template hierarchical intrafibrillar apatite assembly in reconstituted collagen in the presence of polyacrylic acid to sequester calcium and phosphate into transient amorphous nanophases. The use of polyphosphate without a sequestration analogue resulted only in randomly-oriented extrafibrillar precipitations along the fibrillar surface. Conversely, the use of polyacrylic acid without a templating analogue resulted only in non-hierarchical intrafibrillar mineralisation with continuous apatite strands instead of discrete crystallites. The ability of using simple non-protein molecules to recapitulate different levels of structural hierarchy in mineralised collagen signifies the ultimate simplicity in Nature's biomineralisation design principles and challenges the need for using more complex recombinant matrix proteins in bioengineering applications.

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Section: Introductionmentioning

confidence: 99%

Hierarchical and non-hierarchical mineralisation of collagen

et al. 2011

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“…Inorganic polyphosphates play an important role in guiding the nucleation and growth of apatite within the gap zones of collagen fibrils during biomineralization [14]. Sodium trimetaphosphate (STMP, Na 3 P 3 O 9 ), which has been frequently employed as a chemical phosphorylating reagent in the food industry [15–17], has the potential for phosphorylating type I collagen [18]. Chemical phosphorylation of collagen has been shown to be a possible strategy for directing biomimetic growth of bone-like apatite in simulated body fluid (SBF) [19].…”

Section: Introductionmentioning

confidence: 99%

The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin

Liu

et al. 2011

Dental Materials

100

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Objectives-This study examined the use of sodium trimetaphosphate (STMP) as a biomimetic analog of matrix phosphoproteins for remineralization of artificial carious-affected dentin.Methods-Artificial carious lesions with lesion depths of 300±30 µm were created by pHcycling. 2.5% hydrolyzed STMP was applied to the artificial carious lesions to phosphorylate the partially-demineralized collagen matrix. Half of the STMP-treated specimens were bonded with One-Step. The adhesive and non-adhesive infiltrated specimens were remineralized in a Portland cement-simulated body fluid system containing polyacrylic acid (PAA) to stabilize amorphous calcium phosphate as nanoprecursors. Micro-computed tomography (micro-CT) and transmission electron microscopy (TEM) were used to evaluate the results of remineralization after a 4-month period.Results-In absence of PAA and STMP as biomimetic analogs (control groups), there was no remineralization irrespective of whether the lesions were infiltrated with adhesive. For the STMPtreated experimental groups immersed in PAA-containing simulated body fluid, specimens without adhesive infiltration were more heavily remineralized than those infiltrated with adhesive. Statistical analysis of the 4-month micro-CT data revealed significant differences in the lesion Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public AccessAuthor Manuscript Dent Mater. Author manuscript; available in PMC 2012 May 1. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript depth, relative mineral content along the lesion surface and changes in ΔZ between the nonadhesive and adhesive experimental groups (p<0.05 for all the three parameters). TEM examination indicated that collagen degradation occurred in both the non-adhesive and adhesive control and experimental groups after 4 months of remineralization.Significance-Biomimetic remineralization using STMP is a promising method to remineralize artificial carious lesions particularly in areas devoid of seed crystallites. Future studies should consider the incorporation of MMP-inhibitors within the partially-demineralized collagen matrix to prevent collagen degradation during remineralization.

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“…Cyclic trimetaphosphate acts as a functional link in pre-biotic chemistry as a phosphorylation agent of bio-organic molecules (Ozawa et al ., 2004). Sodium trimetaphosphate (Na 3 P 3 O 9 ) is a chemical phosphorylation reagent for food proteins (Lee et al ., 2005; Leone et al ., 2008) and type I collagen (Gunasekaran, 2003). However, in the absence of an agent to stabilize ACP, the use of as a matrix phosphoprotein analog for collagen mineralization Na 3 P 3 O 9 resulted only in the large extrafibrillar mineral spheres deposited around the collagen matrix (Li and Chang, 2008).…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Analogs for Collagen Biomineralization

Kim

Liu

et al. 2010

J Dent Res

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Inability of chemical phosphorylation of sodium trimetaphosphate to induce intrafibrillar mineralization of type I collagen may be due to the failure to incorporate a biomimetic analog to stabilize amorphous calcium phosphates (ACP) as nanoprecursors. This study investigated adsorption/desorption characteristics of hydrolyzed and pH-adjusted sodium trimetaphosphate (HPA-Na3P3O9) to collagen. Based on those results, a 5-minute treatment time with 2.8 wt% HPA-Na3P3O9 was used in a single-layer reconstituted collagen model to confirm that both the ACP-stabilization analog and matrix phosphoprotein analog must be present for intrafibrillar mineralization. The results of that model were further validated by complete remineralization of phosphoric-acid-etched dentin treated with the matrix phosphoprotein analog and lined with a remineralizing lining composite, and with the ACP-stabilization analog supplied in simulated body fluid. An understanding of the basic processes involved in intrafibrillar mineralization of reconstituted collagen fibrils facilitates the design of novel tissue engineering materials for hard tissue repair and regeneration.

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New phosphorylated derivatives of carboxymethylcellulose with osteogenic activity

Cited by 41 publications

References 25 publications

Hierarchical and non-hierarchical mineralisation of collagen

Hierarchical and non-hierarchical mineralisation of collagen

The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin

Biomimetic Analogs for Collagen Biomineralization

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