New Principles of Ion-Exchange Techniques Suitable to Sample Preparation and Group Separation of Natural Products Prior to Liquid Chromatography

Bjerg, Birthe; Olsen, Ole H.; Rasmussen, Kim Wedel; S⊘rensen, Hilmer

doi:10.1080/01483918408073996

Cited by 59 publications

(25 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HPLC lines for sinapine were performed according to a modified method of Clausen et al (1983) under isocratic conditions as described by Clausen et al (1985). The extraction of sinapine was achieved with 70% methanol as recommended by Bjerg et al (1984).…”

Section: Methodsmentioning

confidence: 99%

Anti-nutritional factors in canola produced in the Western and Southern Cape areas of South Africa

Brand¹,

Smith²,

Hoffman³

2008

SA J. An. Sci.

View full text Add to dashboard Cite

The development of low erucic acid, low glucosinolate cultivars of canola seed has led to the availability of a feed ingredient with considerable potential to replace soyabean meal in diets for all classes of farm animals. The sinapine and glucosinolate content of various canola cultivars cultivated in two areas of the Western Cape, South Africa were compared. There were no significant differences in sinapine content between the canola produced in the Western and Southern Cape (mean value of 9.95 mg sinapine/g grain).There were cultivar differences, with Varola 54 and Rainbow cultivars having significantly higher sinapine concentrations than Varola 50. There were no significant differences between the aliphatic, indolyl or aromatic glucosinolate content of the canola originating from either the Swartland or the Rûens areas in South Africa (mean value of 17.84 µmol total glucosinolates/g grain). There were significant differences in the total glucosinolate content of the various canola cultivars. Varola 44 and Hylite 200TT had the lowest total glucosinolate concentration and Varola 50 had the highest glucosinolate concentration. The results obtained seem to indicate a maximum dietary inclusion level of up to 14% for South African produced canola with an average of 17.83 µmol glucosinolates per gram to ensure optimal animal production.

show abstract

Section: Methodsmentioning

confidence: 99%

Anti-nutritional factors in canola produced in the Western and Southern Cape areas of South Africa

Brand¹,

Smith²,

Hoffman³

2008

SA J. An. Sci.

View full text Add to dashboard Cite

show abstract

“…Sinapine was extracted from defatted flours with 70% methanol as recommended by Bjerg et al [19] for 30 min at 75˚C and the samples were then centrifuged The HPLC analysis for sinapine was basically the method described by Clausen et al [20] slightly modified by us. Sinapine was separated by isocratic HPLC and detection at 330 nm.…”

Section: Extraction and Separation Of Sinapinementioning

confidence: 99%

Glucosinolates and Sinapine in Camelina Meal

Russo¹,

Reggiani²

2017

FNS

View full text Add to dashboard Cite

Forty seven accessions of camelina (Camelina sativa L. Crantz) were analyzed for glucosinolates (GSLs) and sinapine in defatted meal. These antinutritional compounds are undesirable in camelina meal for use in animal feeding and therefore we show their variability to identify the best varieties for future breeding programs. Total GSLs ranged from 19.6 to 40.3 mmol Kg −1 dry weight (DW) with an average of 30.3 mmol kg −1 DW. Great variability has also been observed in the levels of individual GSLs (GSL1, GSL2 and GSL3), so that the content of GSL1 and GSL3 were not correlated to each other in the accessions of camelina. Five out of six winter forms of camelina showed low content of GSLs. Sinapine ranged from 1.09 to 4.75 g Kg −1 DW with an average of 2.57 g kg −1 DW. The sinapine content was not correlated with that of GSLs. The use of camelina meal is only limited by the presence of GSLs while sinapine content can be ignored in camelina varieties.

show abstract

“…The extraction of sinapine was achieved with 70% (v/v) methanol as recommended by Bjerg et al [23]. The centrifuged extract was diluted and then injected without further purification [24].…”

Section: Determination Of Sinapinementioning

confidence: 99%

Effect of dehulling on the composition of antinutritive compounds in various cultivars of rapeseed

Matthäus¹

1998

Fett/Lipid

View full text Add to dashboard Cite

show abstract

New Principles of Ion-Exchange Techniques Suitable to Sample Preparation and Group Separation of Natural Products Prior to Liquid Chromatography

Cited by 59 publications

References 13 publications

Anti-nutritional factors in canola produced in the Western and Southern Cape areas of South Africa

Anti-nutritional factors in canola produced in the Western and Southern Cape areas of South Africa

Glucosinolates and Sinapine in Camelina Meal

Effect of dehulling on the composition of antinutritive compounds in various cultivars of rapeseed

Contact Info

Product

Resources

About