Denmark. Seeds ofEruca vesicaria (L.) Cav. em. Thell. ssp. sutiva Thell. were obtained from (RAHMAN et JI. 1986). Seeds ot' Crumbc riruritimu L. were collected troni the natural sites in Denmark. Seeds ofEruca vesicaria (L.) Cav. em. Thell. ssp. sativa Thell. were obtained from U S . and multiplied by growing of the plants at Hojbakkegaard, Taastrup, Denmark. Seeds of Iberis amara L., Tropaeolum majm L. cv. Grandiflorum and Cheiruntus cheiri L. were purchased from J. E. Ohlsens Enke A/S, Taastrup, Denmark. Seeds of Sinapis alba L. were obtained from Trifolium-Silo A/S, Taastrup, Denmark. General methods and instrumentationMethods and equipment used for paper chromatography (PC), high voltage electrophoresis (HVE), 'H-and I3C-NMR have been described previously (OLSEN and SORENSEN 1980). The glucosinolate analysis was performed on intact glucosinolates and desulfoglucosinolates using reversed phase high performance liquid chromatography (HPLC) as described elesewhere (BJERG and SORENSEN 1987a). Isolation of intact glucosinolatesThe techniques used for isolation and characterization of the glucosinolates have followed the principles described previously (BJERG and S~R E N S E N 1987 b). Seeds (100 g) were homogenized with an Ultra-Turrax homogenizer in boiling methanol: water (7: 3; 0.6 1) cooled and filtered. The residue was treated twice in this way and the combined filtrates were concentrated in vacuum to about 30 ml and extracted with 2 x 30 ml chloroform. After centrifugation the water phase was transferred to a column ofAmberlite IR-120 (H+; 5 x 40 cm) connected in series to a column ofEcteola-cellulose (AcO-; 5 x 100 cm). The two columns were flushed with water (2 1) after which the glucosinolates were eluted from the Ecteola column with 1 M pyridine. Glucosinolate containing fractions were pooled, concentrated and transferred to a column ofAmberlite IR-120 (K+; 5 x 40 cm). The column was flushed with water and the fractions with potassium salts of the glucosinolates were take to dryness before final purification on Sephadex, Polyclar AT and/or SPE columns as described elsewhere (BJERG and SORENSEN 1987 b). The purity of the isolated glucosinolates was determined by the previously described methods (BJERG and SORENSEN 1987b) including PC, HVE, HPLC, 'Hand 13C-NMR. In this way seeds of C. maritima (83 g) afforded epiprogoitrin (4.6 9); seeds of E. vesicaria (100 g) afforded glucoraphanin (2.8 g), glucoerucin and lower amounts of some other glucosinolates; seeds of B. cumpestris cv. Sampad (200 g) afforded gluconapin (5.9 8); seeds of I. urnara (200 g) afforded glucoiberin (12 g); seeds of T. majzs cv. grandiflorum (200 g) afforded glucotropaeolin (8 g); seeds of C. cheiri (200 g) afforded glucocheirolin (10 8).The isolated crystalline glucosinolates were stored under vacuum in a desiccator until use. Preparation of myrosinasesThe thioglucoside glucohydrolase EC 3.2.3.1 (myronisases) were extracted from seeds of Sinapis ulba L. according to the procedure of BILLE et al. (1983 b), with centrifugations carried...

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Ten mbred lines of tabe beans (Vicia {aha L.) selected according to their quality characters have been investigated for carbohydrates, proteins and antinutritional compounds. Digestible energy, Nbaiance trials with growing rats comprising determination of the protein digestibility and biological value were used as criteria in connection -n-ith comprehensive chemical-biochemical analysis.The chemical composition of the ten lines show-ed a considerable diversity as did the results from the rat trials. However, the content of i-icine and convieine in all of the investigated lines were below the level previously tound to have effect,^ on the nutritive value. Starch, protein and fibre were the quantitativeh-dominating seed constituents, and all showed great variation among the lines.The starch content was not correlated to the quaiity or nutritive value of the seed, whereas the prote-in content was negatively correlated to the biological value and net protein utilization. These correlations followed the content of essential amino acids, lysine, threonine .and methionine in the faba bean proteins. The content of the sulfur-containing amino acids cysteine and methionine are especially dominant factors for protein quality. A simple method for total sulfur determination was found nor to be a sufficiently reliable technique for evaluating the content of methionine and cysteine in the faba beans.Tannin, insoluble-and total dietary fibre are pnenolie aromatic compounds which were negatively eorrelated with the faba bean quality as expressed by digestible energy, the protein digestibility and the biological value of the faba beans. The results obtained have also revealed, that it is not sufficient to consider tannin as a group m relation to the faba bean quality. We need to separate and evaluate the different types of phenolics in relation to the variations in quality of faba beans. Some of the low molecular weight (LMW) phenolics in the faba beans seem to be involved in inhibitor)' effects on hydrolase enzymes, chymotr}'psin and try-psm. Trypsin and chymotrypsin from different animals were difterent in their sensitivity" to faba bean inhibitors and additional experiments are required to reveal details about these effects. It l:ias .also been revealed that iTuet-osans and LMW' carbohydrates (oligosaccharides) are important in relation to the quality of faba beans.Key words: Vieia faba -inbred lines -nutritive value -antinutritional compounds -protein quality -vieine -convieine -tannin -hydrolase inhibitors -trypsin -chymotrypsin -carbohydrates -fibres -fructosans -oligosaccharidesstarch Faba bean varieties h.ave a high yield capacity compared to other grain legumes (HA^STIN and STE^S-ART 1979, DANTUMA et al 1983), Thencarbo-hydrate content is high, but the nutritive value and quality are negatively affected by antinutritional and/or toxic compounds which occur m too-high concentrations m some faba bean lines. In addition, the content ol sulfurcontaming ammo acids is very low (BJERG et al, 1984 a, BJERG et al, 1984 c and ref...