New role of phenylpropanoid compounds during sugarcane micropropagation in Temporary Immersion Bioreactors (TIBs)

An Approach for Micropropagation of Blueberry (<i>Vaccinium corymbosum</i> L.) Plants Mediated by Temporary Immersion Bioreactors (TIBs)

“…In this paper, CO 2 concentrations in TIBs were similar to those reported for sugarcane [19,21]; however, both sucrose reduction and luminosity were dissimilar according to the blueberry genotypes, and to the laboratory facilities, respectively. Considering photosynthesis as a complex process involving a range of environmental factors determining carbon assimilation, these results should be the tip of the iceberg toward further research on V. corymbosum (L.) micropropagation in TIBs, in this case as model specie of shrub crops.…”

“…Altogether, results demonstrate the plasticity of the TIBs technology to manage the major environmental factors toward an improvement of plant physiological processes, in this case the in vitro photosynthesis. Previously both the enhancement of the plant-air contact in a 0.4 MPa CO 2 enrichment atmosphere and placement under a light intensity of 110 mM m −2 ·s −1 were significant factors to improve the phenolic metabolites secretion in sugarcane multiplication in TIBs [19]. The differential expression of Rubisco transcripts in increased CO 2 concentration in parallel with a reduction of sucrose in the culture medium indicated the change from a heterotrophic to a photomixotrophic metabolic stage in vitro plantlets micropropagated in TIBs [21].…”

“…Because the high production costs render conventional micropropagation systems (agar-based) less suitable for large-scale production, liquid cultures and automation has the potential to resolve the manual handling of the various stages of micropropagation [15]. Bioreactor cultures also permit an additional management of both physical and chemical environments, such as air exchange, photosynthetic photon flux, and CO 2 content, resulting in more efficient plant performance during the in vitro micropropagation [19][20][21].…”

Sugarcane metabolites produced in CO2-rich temporary immersion bioreactors (TIBs) induce tomato (Solanum lycopersicum) resistance against bacterial wilt (Ralstonia solanacearum)

“…The use of bioreactors has led to the development of technologies suitable for large-scale in vitro plant propagation. Currently, various plant species are propagated using bioreactors for biomass production as well as large-scale micropropagation (Escalona et al amount of secondary metabolites, mostly phenolic compounds, has been observed in sugarcane micropropagated in TIBs (Arencibia et al 2008). It is known that physical wounding (such as those produced during plant culture manipulation) triggers several important changes in plant metabolism.…”

Cardiotonic glycosides from biomass of Digitalis purpurea L. cultured in temporary immersion systems

“…Temporary immersion system (TIS) is a cheap technology for automation of the processes of in vitro plant tissue culture and has been employed in several cultures for production of microtubers (Jiménez et al 1999;Cabrera et al 2005), somatic embryos (Niemenak et al 2008) and shoots (Roels et al 2005;Hajari et al 2008;Arencibia et al 2008;Yang and Yeh 2008). Due to the facilities of TIS for large scale culturing of plant organs, it also represents an attractive alternative for the production of plant secondary metabolites, avoiding the disadvantages of other techniques such as standard bioreactors.…”