New roles for CDC25 in growth control, galactose regulation and cellular differentiation in Saccharomyces cerevisiae

Abstract

Living organisms display large differences in stress resistance throughout their life cycles. To study the coordinated regulation of development and stress responses in exponentially growing yeast, mutants that displayed elevated heat-shock resistance at this stage were screened for. Here, two new mutant alleles of CDC25 in Saccharomyces cerevisiae, cdc25-21 and cdc25-22, are described. During exponential growth in glucose at 25 6C, these mutants are resistant to heat, oxidative, osmotic and ionic shock, accumulate stress-protein transcripts, show slow growth rates, thick cell walls and glycogen hyperaccumulation and lack cAMP signalling in response to glucose. Genetic and cellular analyses revealed that the stationary-phase phenotypes of cdc25-21 and cdc25-22 mutants are not due to entrance to a G 0 state during exponential growth, but are the result of a prolonged G 1 phase. It was found that, in the W303 background, CDC25 is dispensable for growth in glucose media. However, CDC25 is essential for growth in galactose, in non-fermentable carbon sources and under continuous incubation at 38 6C. In conclusion, the function of the catalytic, C-terminal domain of Cdc25p is not only important for fermentative growth, but also for growth in non-fermentable carbon sources and to trigger galactose derepression.

CDC25 protein expression and interaction with DAZL in human corpus luteum

“…The results of this study are in agreement with recent studies that revealed the existence of dual-function regulators that can participate in controlling both cell proliferation and differentiation (50). In Saccharomyces cerevisiae, CDC25 has been found to contribute growth control galactose regulation and cellular differentiation (51). In Caenorhabditis elegans, DNA damage checkpoint proteins have been found to control survival of the postmitotic cells (52).…”

Wee1 and Cdc25 control morphogenesis, virulence and multistress tolerance of Beauveria bassiana by balancing cell cycle‐required cyclin‐dependent kinase 1 activity

“…The common functions of Wee1 and Cdc25 in the yeasts are also found in the smut fungus Ustilago maydis, in which both the Wee1 overexpression and the Cdc25 inactivation led to specific G2 arrest that correlated with the high level of Cdk1 phosphorylation at Tyr 15 (Sgarlata and Pérez-Martín, 2005a,b). Aside from an involvement in cell cycle, Cdc25 has proved to participate in the regulation of yeast cell responses to heat, oxidation, high osmolarity and ionic shock (Folch-Mallol et al, 2004).…”

Discovering pathways by orienting edges in protein interaction networks

“…Another cascade starts with Ras2 and Cdc25 instead of Rga1. These proteins work together and are important for the exit from a G0 state (52). Along with Cdc28 they allow the G1/S transition by increasing Cln2 levels (53).…”