New small-scale cross-axis coil planet centrifuge

Shinomiya, Kazufusa; Kobayashi, Hiroko; Inokuchi, Norio; Kobayashi, Kōji; Oshima, Hisashi; Kitanaka, Susumu; Yanagidaira, Kazuhiro; Sasaki, Haruo; Muto, Minoru; Okano, M.; Ito, Yoichiro

doi:10.1016/j.chroma.2007.02.116

Cited by 13 publications

(9 citation statements)

References 17 publications

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“…When using an aqueous-aqueous polymer phase system composed of 16.0% (w/w) PEG 1000 – 6.3% (w/w) dibasic potassium phosphate — 6.3% (w/w) monobasic potassium phosphate (pH 6.6) with upper phase mobile, the type I-A sample gave a small impurity peak near the solvent front (Fig. 2A) as observed in the previous study [12]. The purity of RNase A in the type I-A sample was calculated at almost 88.0% from the chromatogram.…”

Section: Resultssupporting

confidence: 76%

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Countercurrent chromatographic separation and purification of various ribonucleases using a small-scale cross-axis coil planet centrifuge with aqueous–aqueous polymer phase systems

Shinomiya

Kobayashi

Motoyoshi

et al. 2009

Journal of Chromatography B

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Countercurrent chromatographic separation and purification of various ribonucleases (RNases) was performed using small-scale cross-axis coil planet centrifuge (X-axis CPC) with aqueous-aqueous polymer phase systems. RNases B and A were well resolved from each other with an aqueous-aqueous polymer phase system composed of 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate (pH 9) as the lower phase mobile. The commercial RNase A samples obtained from three different companies were also highly purified using the 16.0% (w/w) PEG 1000 – 6.3% (w/w) dibasic potassium phosphate — 6.3% (w/w) monobasic potassium phosphate system (pH 6.6) using the upper phase as the mobile phase. Recombinant RNase Po1, an RNase T1 family enzyme, was further successfully separated from the crude extract using the same solvent system with the lower phase used as the mobile phase. The RNase activities were well preserved during the CCC separation. The overall results demonstrate that small-scale X-axis CPC is useful for a simple and rapid purification of various RNases in a preparative-scale

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Section: Resultssupporting

confidence: 76%

“…In our previous studies [12], a small amount of impurity was found as a single peak in the CCC separation of the commercial RNase sample (type I-A produced by Sigma). This result indicates that the commercial RNase A sample may contain some impurity.…”

Section: Resultsmentioning

confidence: 99%

Countercurrent chromatographic separation and purification of various ribonucleases using a small-scale cross-axis coil planet centrifuge with aqueous–aqueous polymer phase systems

Shinomiya

Kobayashi

Motoyoshi

et al. 2009

Journal of Chromatography B

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“…The design and fabrication of the apparatus and the column configuration were previously described in detail [8, 9]. …”

Section: Methodsmentioning

confidence: 99%

“…In our laboratory, the floor model of the cross-axis CPC has been built and successfully applied to the protein separation using aqueous-aqueous polymer phase system [7]. Recently, a new-small-scale cross-axis CPC has been further developed in our laboratory to improve the partition efficiency [8, 9]. This small-scale cross-axis CPC is a compact model where four coiled columns of similar weight are mounted symmetrically around the rotary frame to achieve stable balancing of the centrifuge system even under a high revolution speed.…”

Section: Introductionmentioning

confidence: 99%

Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns

et al. 2016

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1) Background Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of partition efficiency and shortening theseparation time. 2) Methods The locular multilayer coils modified with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl (MU) sugar derivatives using the small-scale cross-axis coil planet centrifuge. 3) Results Proteins were well separated from each other and the separation was improved at a low flow rate of the mobile phase. On the other hand, 4-MU sugar derivatives were sufficiently resolved with short separation time at a highflow rate of the mobile phase under satisfactory stationary phase retention. 4) Conclusion Effective separations were achieved using the locular multilayer coil for proteins with aqueous-aqueous polymer phase systems and for 4-MU sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule.

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“…The details of the design and rotation mechanism of this apparatus were described in our previous publications. 10,21 Two different types of column holders, the circular and the elliptic cylindrical column holders, were used in the present study as illustrated in Fig. 1.…”

Section: Apparatus and Column Holdersmentioning

confidence: 99%

Partition Efficiency of Eccentric Coil for Countercurrent Chromatographic Separation of Proteins Using Small-scale Cross-axis Coil Planet Centrifuge with Circular and Elliptic Cylindrical Columns

et al. 2020

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The partition efficiency of the double-spaced coil for eccentric and toroidal coils on countercurrent chromatographic separation of proteins was evaluated using the small-scale cross-axis coil planet centrifuge (CPC) equipped with circular and elliptic cylindrical columns. Standard cytochrome C, myoglobin and lysozyme samples were used for separation with the 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate system. In the circular column, the double-spaced eccentric coil yielded better peak resolution than the double-spaced toroidal coil, and the double-spaced eccentric coil than the single-spaced eccentric coil. In the elliptic column, the double-spaced eccentric coil also produced better peak resolution than the double-spaced toroidal coil, but the single-spaced eccentric coil than the double-spaced eccentric coil. The overall results indicated that the double-spaced eccentric coil for the circular column and the single-spaced eccentric coil for the elliptic column yielded better protein separation using the small-scale cross-axis CPC with aqueous two-phase solvent systems.

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New small-scale cross-axis coil planet centrifuge

Cited by 13 publications

References 17 publications

Countercurrent chromatographic separation and purification of various ribonucleases using a small-scale cross-axis coil planet centrifuge with aqueous–aqueous polymer phase systems

Countercurrent chromatographic separation and purification of various ribonucleases using a small-scale cross-axis coil planet centrifuge with aqueous–aqueous polymer phase systems

Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns

Partition Efficiency of Eccentric Coil for Countercurrent Chromatographic Separation of Proteins Using Small-scale Cross-axis Coil Planet Centrifuge with Circular and Elliptic Cylindrical Columns

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