New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids

Covalently immobilized chemically modified lysozyme as a sorbent for bacterial endotoxins (lipopolysaccharides)

“…Chicken egg lysozyme is the most well-known and the most used bacteriolytic enzyme in modern medicine and biotechnology. In our recent work we have shown that immobilized lysozyme can not only destroy bacteria but also effectively adsorbs the endotoxins (bacteria lipopolysaccharides) [3]. Endotixin adsorbtion is required for the extracorporeal treatment of sepsis [4,5].…”

“…Endotixin adsorbtion is required for the extracorporeal treatment of sepsis [4,5]. In our recent work it was also shown that the new sorbent is blood-compatible so it can potentially be used in extracorporeal therapy technologies to remove pathogenic components from the patient’s bloodstream [3,6]. The practice of using extracorporeal therapy in the treatment of severe diseases including sepsis has significantly expanded in recent years [4,5,7].…”

“…The practice of using extracorporeal therapy in the treatment of severe diseases including sepsis has significantly expanded in recent years [4,5,7]. In addition we have shown that immobilized lysozyme absorbs not only endotoxins but also a certain amount of immunoglobulin G (IgG) [3]. Binding to IgG may explain the possible additional physiological role of lysozyme as opsonin [3,8], but this binding is not required in the treatment of sepsis.…”

Lipopolysaccharide increases lysozyme adhesion to chitin

“…More recently, a group of Moscow biochemists [8] found that lysozyme binds to the intestinal endotoxin lipopolysaccharide, which is secreted by gram-negative bacteria and has a very strong pro-inflammatory effect [9]. On this basis, they proposed the use of immobilized lysozyme for sorbtion of lipopolysaccharide from the blood of patients with sepsis [8].…”

Preventive Effect on the Gingiva of Rats Phytogel “Lysozyme-Forte” With Indomethacin Intoxication

“…О. В. Богатського НАН України» (м. Одеса) 2 Харківський національний медичний університет 3 Державна установа «Інститут стоматології та щелепно-лицевої хірургії Національної академії медичних наук України» Лізоцим, як один з найбільш важливих факторів неспецифічного імунітету, знайшов широке застосування в медицині в якості лікувальнопрофілактичного засобу [1,2]. Розроблена велика кількість лізоцимвмісних лікарських препаратів та гігієнічних засобів для використання в стоматології [3][4][5].…”