New spectrophotometric method for simultaneous determination of metoprolol tartarate and hydrochlorthiazide in tablets

Abstract

The present work describes a two-wavelength method for simultaneous determination of metoprolol and hydrochlorthiazide in fixed dose combination tablet. The wavelengths selected for method were 257.8 nm, 282.9 nm and 315.0 nm. The absorbance difference at first two wavelengths was used for determination of metoprolol and the latter was used for determination of hydrochlorthiazide. The recovery value for the drugs from the tablet matrix was found to be 100.55% (metoprolol) and 99.97% (comparison with standard) and 98.09% (E1%, 1cm) for hydrochlorthiazide. The method has an advantage that hydrochlorthiazide can be estimated in combination, as there is no interference of metoprolol at 315.0 nm. The method was evaluated statistically for its accuracy and precision.

Physico-Chemical and Analytical Study of Metoprolol Tartrate: An Antihypertensive Drug

“…Combination is official in the United States Pharmacopoeia which recommended liquid chromatographic assay with two different chromatographic conditions for each of drugs [3]. Various methods have been reported in the literature for simultaneous determination of metoprolol and hydrochrothizen in tablet dosage forms which includes spectrophotometry [4][5][6][7][8][9], high performance liquid chromatography [8][9][10][11][12][13][14][15] and electrophoresis [16]. Chemistry;Vol.…”

An Improved Rapid HPLC Method for the Separation of Five Anti-Hypertensive Agents Using C18 Column: Application to Hydrochlorothiazide Determination in Bulk and Tablet Dosage Form

“…Literature survey reveals that for the determination of above said five anti-hypertensive agents in biological fluids like plasma, blood, urine and pharmaceutical dosage forms by spectrophotometry [1][2][3][4] , spectrofluorimetry [5] , TLC [6][7] , HPTLC [8] , High Performance Liquid Chromatography (RP-HPLC) with UV detection [9][10][11][12][13][14][15][16][17][18] , fluorimetric detection [19] , ion-pair HPLC [20] , RP-UPLC [21] and Liquid Chromatography-Mass Spectrometry [22][23] , Capillary electrophoresis [24] .In fact there is a need for the development of a novel, simple, rapid, efficient RP-HPLC analytical method with reproducibility for determination of HCT in bulk and pharmaceutical dosage forms. A group of five anti-hypertensive drugs investigated in the present study are listed in Figure 1.…”

Isocratic Separation of Four Beta Blockers With Amlodipine by C18 Rp-Hplc: Application to Amlodipine Determination in Pharmaceutical Dosage Forms

“…NEBI is chemically1-(6-fluorochroman-2-yl)-{[2-(6-fluorochroman-2-yl)-2-hydroxy ethyl] amino} ethanol ( Figure 1e). Literature survey reveals that for the determination of above said five anti-hypertensive agents in biological fluids like plasma, blood, urine and pharmaceutical dosage forms by spectrophotometry [1][2][3][4] , spectrofluorimetry 5 , TLC [6][7] , HPTLC [8][9] , High Performance Liquid Chromatography (RP-HPLC) with UV detection [10][11][12][13][14][15][16][17][18][19][20][21] , fluorimetric detection 22 , ion-pair HPLC 23 , RP-UPLC 24 and Liquid Chromatography-Mass Spectrometry [25][26] . As a matter of fact there is a need for the development of a novel, simple, rapid, efficient RP-HPLC analytical method with reproducibility for determination of AMLO in bulk and pharmaceutical dosage forms.…”