New syntheses of 1d- and 1l-1,2-anhydro-myo-inositol and assessment of their glycosidase inhibitory activities

Falshaw, Andrew; Hart, Joanne B.; Tyler, Peter C.

doi:10.1016/s0008-6215(00)00192-0

Cited by 30 publications

(13 citation statements)

References 20 publications

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“…Terpene metabolism was also modified according to our data, with increased abundance of transcripts for terpene synthases VIT_18s0001g04120 and VIT_00s0692g00020, while reducing that of 10 others with VIT_19s0014g04930 and VIT_00s0271g00010 most intensively. Enrichment of compounds known to inhibit pathogen growth and biofilm formation was also detected in our metabolome data such as erythritol and 2-deoxyerythritol ( Ghezelbash et al, 2012 ), 1,2-anhydro-myo-inositol and arbutin; these latter glycosidase and tyrosinase inhibitors, respectively ( Falshaw et al, 2000 ; Supplementary Table S10 and Figure 7 ). The pathogen might also benefit from increased concentration of nutritional compounds such as fructose, tryptophan, and glutamine.…”

Section: Resultssupporting

confidence: 57%

Molecular Profiling of Pierce’s Disease Outlines the Response Circuitry of Vitis vinifera to Xylella fastidiosa Infection

et al. 2018

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Pierce’s disease is a major threat to grapevines caused by the bacterium Xylella fastidiosa. Although devoid of a type 3 secretion system commonly employed by bacterial pathogens to deliver effectors inside host cells, this pathogen is able to influence host parenchymal cells from the xylem lumen by secreting a battery of hydrolytic enzymes. Defining the cellular and biochemical changes induced during disease can foster the development of novel therapeutic strategies aimed at reducing the pathogen fitness and increasing plant health. To this end, we investigated the transcriptional, proteomic, and metabolomic responses of diseased Vitis vinifera compared to healthy plants. We found that several antioxidant strategies were induced, including the accumulation of gamma-aminobutyric acid (GABA) and polyamine metabolism, as well as iron and copper chelation, but these were insufficient to protect the plant from chronic oxidative stress and disease symptom development. Notable upregulation of phytoalexins, pathogenesis-related proteins, and various aromatic acid metabolites was part of the host responses observed. Moreover, upregulation of various cell wall modification enzymes followed the proliferation of the pathogen within xylem vessels, consistent with the intensive thickening of vessels’ secondary walls observed by magnetic resonance imaging. By interpreting the molecular profile changes taking place in symptomatic tissues, we report a set of molecular markers that can be further explored to aid in disease detection, breeding for resistance, and developing therapeutics.

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Section: Resultssupporting

confidence: 57%

Molecular Profiling of Pierce’s Disease Outlines the Response Circuitry of Vitis vinifera to Xylella fastidiosa Infection

et al. 2018

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“…34 The data set for CBE cannot be used for fitting because CBE is an irreversible inhibitor, but the observed inhibition is consistent with microtiter plate readings (cf., Figure S13). 37 …”

Section: Resultsmentioning

confidence: 99%

A Fully Unsupervised Compartment-on-Demand Platform for Precise Nanoliter Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition

et al. 2013

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The ability to miniaturize biochemical assays in water-in-oil emulsion droplets allows a massive scale-down of reaction volumes, so that high-throughput experimentation can be performed more economically and more efficiently. Generating such droplets in compartment-on-demand (COD) platforms is the basis for rapid, automated screening of chemical and biological libraries with minimal volume consumption. Herein, we describe the implementation of such a COD platform to perform high precision nanoliter assays. The coupling of a COD platform to a droplet absorbance detection set-up results in a fully automated analytical system. Michaelis–Menten parameters of 4-nitrophenyl glucopyranoside hydrolysis by sweet almond β-glucosidase can be generated based on 24 time-courses taken at different substrate concentrations with a total volume consumption of only 1.4 μL. Importantly, kinetic parameters can be derived in a fully unsupervised manner within 20 min: droplet production (5 min), initial reading of the droplet sequence (5 min), and droplet fusion to initiate the reaction and read-out over time (10 min). Similarly, the inhibition of the enzymatic reaction by conduritol B epoxide and 1-deoxynojirimycin was measured, and Ki values were determined. In both cases, the kinetic parameters obtained in droplets were identical within error to values obtained in titer plates, despite a >104-fold volume reduction, from micro- to nanoliters.

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“…In order to evaluate the sensitivity of the assay, the inhibitor conduritol β-epoxide (Falshaw et al, 2000) was spotted in decreasing amounts onto the TLC layer and a minimum detection limit of 0.1 µg was observed. The method was compatible with different TLC stationary phases, each showing clear spots indicating the presence of the inhibitor in all cases; however, the detection limit was different for the different supports (Table 1).…”

Section: Resultsmentioning

confidence: 99%

A rapid TLC autographic method for the detection of glucosidase inhibitors

Salazar

Furlán

2007

Phytochemical Analysis

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New syntheses of 1d- and 1l-1,2-anhydro-myo-inositol and assessment of their glycosidase inhibitory activities

Cited by 30 publications

References 20 publications

Molecular Profiling of Pierce’s Disease Outlines the Response Circuitry of Vitis vinifera to Xylella fastidiosa Infection

Molecular Profiling of Pierce’s Disease Outlines the Response Circuitry of Vitis vinifera to Xylella fastidiosa Infection

A Fully Unsupervised Compartment-on-Demand Platform for Precise Nanoliter Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition

A rapid TLC autographic method for the detection of glucosidase inhibitors

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