New Taxanes From Taxus brevifolia

Kingston, David G. I.; Hawkins, Douglas R.; Ovington, Liza G.

doi:10.1021/np50022a019

Cited by 70 publications

(27 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On one hand, extensive experience relating to TCMs may facilitate the identification of novel active substances. This approach has already proven successful by many established cytostatic compounds of natural origin [27], e.g., Vinca alkaloids from Vinca major [28], epipodophyllotoxins from Podophyllum peltatum [29,30], taxanes from Taxus brevifolia [31,32], camptothecin from Camptotheca acuminate [33,34], and so on. On the other hand, TCMs in conjunction with chemotherapy or TCMs alone may be beneficial in terms of improvement in marrow suppression and immune system, and may improve overall state of the quality of life (see review by Zhang et al [6]).…”

Section: Discussionmentioning

confidence: 99%

Baicalin induces human mucoepidermoid carcinoma Mc3 cells apoptosis in vitro and in vivo

Cai

Guan

et al. 2010

Invest New Drugs

View full text Add to dashboard Cite

Mucoepidermoid carcinoma (MEC) is the most common malignant tumor in salivary glands and high-grade MEC in particular demonstrates little response to chemotherapy which has been used largely for palliative treatment of metastatic disease. Baicalin, one of the main active compounds of Scutellaria baicalensis, possesses anti-inflammatory, antioxidant and antitumor properties. In the present study, we investigated the growth inhibiting and apoptosis-inducing effects of baicalin on a highly metastatic human mucoepidermoid carcinoma cell line Mc3 for the first time. Baicalin exerted dose- and time-dependent antiproliferative potential against Mc3 cells as assessed by MTT assay. Baicalin treatment of Mc3 cells resulted in an accumulation of cells at the G₀/G₁ and G₂/M phase with a concomitant decrease in cells processing to S phase as assessed by flow cytometry. Furthermore, baicalin induced apoptosis of Mc3 cells as determined by annexin V binding and PI dual staining, DNA fragmentation, nuclear condensation and in vivo tumor inhabitation. Rhodamine 123 assay indicated that baicalin caused cytotoxicity and induced apoptosis through decreasing the mitochondrial membrane potential in Mc3 cells. Our results suggest that baicalin seems to be very attractive as a new anticancer drug and a potential chemotherapeutic agent against human high-grade mucoepidermoid carcinoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Baicalin induces human mucoepidermoid carcinoma Mc3 cells apoptosis in vitro and in vivo

Cai

Guan

et al. 2010

Invest New Drugs

View full text Add to dashboard Cite

show abstract

“…A wide range of compounds interact with the α and β tubulin subunits and some function as antimitotic agents, inhibiting cellular replication (Jordan and Wilson 2004; by Wall and Wani (Kingston et al 1982;Parekh and Simpkins 1997;Roytta et al 1984;Wani et al 1971), is such a compound with an ability to stabilize polymerized α-β tubulin heterodimers in microtubules and inhibit polymer disassembly (Orr et al 2003;Schiff and Horwitz 1980). Because of the requirement for microtubule rapid disassembly and dynamic behavior during cell division (Reinecke et al 1997), paclitaxel is effective as a cancer treatment drug (Ramalingam and Belani 2008).…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for Analyzing Mitochondrial Movement: Inhibition by Paclitaxel in a Pheochromocytoma Cell Model

Shprung

Gozes

2008

J Mol Neurosci

View full text Add to dashboard Cite

A method was developed to assess mitochondrial movement in the living cell that is dependent, in part, on microtubule and/or associating protein interactions. The leader sequence from cytochrome-c was used to drive DsRed2 fluorescent proteins to accumulate in the mitochondria, thus enabling to follow mitochondrial (cytochrome-c's) movement. For calculating the percentage of mitochondrial movement, an image-processing program was used (ImageJ). Paclitaxel, an antitumor agent, is a potent microtubule-stabilizing agent that increases the stability of tubulin polymers, inhibiting mitosis and mitochondrial activity in dividing cells. Here, we tested whether paclitaxel inhibits mitochondrial movement in pheochromocytoma cells (a neuronal model, when tested in a differentiated state). While a 2-day exposure to paclitaxel resulted in cellular toxicity (measured as inhibition of mitochondrial activity), 2-3 h exposure to paclitaxel were sufficient to inhibit mitochondrial movement as assessed in 10-20-s imaging sessions in living cells. Mitotracker deep-red staining validated the staining obtained with DsRed2-cytochrome-c and identified intact mitochondria. Results showed a significant paclitaxel dose-dependent inhibition of mitochondrial movement. This new method should enable further assessment of microtubule-interacting drugs and other cytoskeletal components for their potential influence of mitochondrial movement as a test for activity and side effects.

show abstract

“…The C-13 side chain was retained based on the knowledge that this unit, and especially the C-2 0 hydroxyl group, is essential to bioactivity [183,184]. These simplified analogs were reported to show reduced activity in inhibition of tubulin depolymerization, but no detailed data were presented.…”

Section: Synthetic Attempts To Make Simplified Paclitaxel Analogsmentioning

confidence: 99%

Recent Progress in the Chemistry and Biology of Paclitaxel (TaxolTM) and Related Taxanes

Zhao

Kingston

2012

Plant Bioactives and Drug Discovery

Self Cite

View full text Add to dashboard Cite

New Taxanes From Taxus brevifolia

Cited by 70 publications

References 6 publications

Baicalin induces human mucoepidermoid carcinoma Mc3 cells apoptosis in vitro and in vivo

Baicalin induces human mucoepidermoid carcinoma Mc3 cells apoptosis in vitro and in vivo

A Novel Method for Analyzing Mitochondrial Movement: Inhibition by Paclitaxel in a Pheochromocytoma Cell Model

Recent Progress in the Chemistry and Biology of Paclitaxel (TaxolTM) and Related Taxanes

Contact Info

Product

Resources

About