1991
DOI: 10.1038/351456a0
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New use of BCG for recombinant vaccines

Abstract: BCG, a live attenuated tubercle bacillus, is the most widely used vaccine in the world and is also a useful vaccine vehicle for delivering protective antigens of multiple pathogens. Extrachromosomal and integrative expression vectors carrying the regulatory sequences for major BCG heat-shock proteins have been developed to allow expression of foreign antigens in BCG. These recombinant BCG strains can elicit long-lasting humoral and cellular immune responses to foreign antigens in mice.

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Cited by 1,405 publications
(1,026 citation statements)
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References 28 publications
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“…However, LM-OVA induced more numbers of annexin V ϩ OVA-specific CD8 ϩ T cells than those induced by BCG-OVA. Although it is not clear whether all annexin V ϩ CD8 ϩ T cells die, it is, however, quite possible that some level of apoptosis might be occurring during BCG-OVA infection, particularly during the peak effector phase (ϳdays [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. Indeed, as is evident in Fig.…”
Section: Discussionmentioning
confidence: 94%
See 1 more Smart Citation
“…However, LM-OVA induced more numbers of annexin V ϩ OVA-specific CD8 ϩ T cells than those induced by BCG-OVA. Although it is not clear whether all annexin V ϩ CD8 ϩ T cells die, it is, however, quite possible that some level of apoptosis might be occurring during BCG-OVA infection, particularly during the peak effector phase (ϳdays [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. Indeed, as is evident in Fig.…”
Section: Discussionmentioning
confidence: 94%
“…BCG-OVA is a previously described recombinant strain (19) engineered with a partial sequence of the OVA gene (codons 230 -359), downstream of the Ag 85B secretion signal (20), under the control of heat shock protein Laboratory of Cellular Immunology, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada 60 promoter (21). Codons 230 -359 of OVA gene encode the SIINFEKL epitope (OVA [257][258][259][260][261][262][263][264] ) and its flanking sequences (22).…”
Section: Bacterial Strainsmentioning
confidence: 99%
“…BCG Pasteur transformed with empty vector pMV261 was used as the control vaccine [37]. BCG strains and M. tuberculosis H37Rv (ATCC 27294) were propagated in Middlebrook 7H9 media supplemented with 10% albumin-dextrose-catalase enrichment (Difco Laboratories) or enumerated on oleic acid-albumin-dextrose-catalaseenriched Middlebrook 7H11 agar.…”
Section: Bacterial Culturementioning
confidence: 99%
“…The 1693 bp PCR-amplified DNA fragment was first cloned into a TA cloning vector (pDK101) and was subsequently moved into the ScaI site of pGOAL19, to generate the knockout construct pAZI0235. In order to convert pMV261 (Stover et al, 1991) into an integrating vector, a MluI-XbaI fragment from pYUB178 (Pascopella et al, 1994) containing att and int sites was used to replace a MluI-XbaI fragment (encompassing oriM) from pMV261. The resulting plasmid was designated pAZI272.…”
Section: Methodsmentioning
confidence: 99%