New Variants of Pseudomonas aeruginosa High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital

Aguilar-Rodea, Pamela; Estrada-Javier, Elia L.; Jiménez-Rojas, Verónica; Gomez-Ramirez, Uriel; Nolasco-Romero, Carolina G.; Rodea, Gerardo E.; Rodríguez-Espino, Benjamín Antonio; Mendoza-Elizalde, Sandra; Arellano, Cesar; López-Marcelino, Beatriz; Zamboni, Daniela de la Rosa; Gamiño-Arroyo, Ana Estela; Mora-Suárez, Rosalia; García, Margarita Torres; Hernández, Isabel Franco; Parra-Ortega, Israel; Campos-Valdéz, Guillermina; Velázquez-Guadarrama, Norma; Rosas‐Pérez, Irma

doi:10.3390/microorganisms10081533

Cited by 6 publications

(5 citation statements)

References 54 publications

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“…The observations of potential high-risk and locally distributed clones underscore the relevance of continued inspection of the population structure in clinical settings in different parts of the world. Although previous studies had showed that high-risk clones were predominant among multidrug-resistance strains (del Barrio-Tofiño et al, 2020;Hu et al, 2021;Teo et al, 2021;Aguilar-Rodea et al, 2022), we found that 50/212 isolates (23.58%) belong to the 18 previously reported high-risk clones, and that the remaining isolates showed high diversity together with other reported STs in all strains.…”

Section: Discussioncontrasting

confidence: 76%

“…One ST298 isolate and one ST446 isolate, both with MDR/XDR phenotype, were identified in different hospitals. In some regional research projects, the clonal diversity of P. aeruginosa is much lower among multidrug-resistant isolates (del Barrio-Tofiño et al, 2020;Hu et al, 2021;Teo et al, 2021;Aguilar-Rodea et al, 2022). This is not entirely the case among our MDR subsets from 2018-2020.…”

Section: Discussionmentioning

confidence: 78%

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Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China

Zhao

Chen

et al. 2023

Front. Microbiol.

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The ever-increasing prevalence of infections produced by multidrug-resistant or extensively drug-resistant Pseudomonas aeruginosa is commonly linked to a limited number of aptly-named epidemical ‘high-risk clones’ that are widespread among and within hospitals worldwide. The emergence of new potential high-risk clone strains in hospitals highlights the need to better and further understand the underlying genetic mechanisms for their emergence and success. P. aeruginosa related high-risk clones have been sporadically found in China, their genome sequences have rarely been described. Therefore, the large-scale sequencing of multidrug-resistance high-risk clone strains will help us to understand the emergence and transmission of antibiotic resistances in P. aeruginosa high-risk clones. In this study, 212 P. aeruginosa strains were isolated from 2 tertiary hospitals within 3 years (2018–2020) in Guangdong Province, China. Whole-genome sequencing, multi-locus sequence typing (MLST) and antimicrobial susceptibility testing were applied to analyze the genomic epidemiology of P. aeruginosa in this region. We found that up to 130 (61.32%) of the isolates were shown to be multidrug resistant, and 196 (92.45%) isolates were Carbapenem-Resistant Pseudomonas aeruginosa. MLST analysis demonstrated high diversity of sequence types, and 18 reported international high-risk clones were identified. Furthermore, we discovered the co-presence of exoU and exoS genes in 5 collected strains. This study enhances insight into the regional research of molecular epidemiology and antimicrobial resistance of P. aeruginosa in China. The high diversity of clone types and regional genome characteristics can serve as a theoretical reference for public health policies and help guide measures for the prevention and control of P. aeruginosa resistance.

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Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 78%

Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China

Zhao

Chen

et al. 2023

Front. Microbiol.

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“…However, the association between ICs and STs, which could make the assignment easier, was established by computational, rather than molecular, methods, with the introduction of the eBURST algorithm, which divides an MLST data set of any size into groups of related isolates and CCs and predicts the founding (ancestral) genotype of each CC [ 58 ]. This algorithm was widely used for defining CCs of various bacterial species [ 59 , 60 ], and it was later applied to associate A. baumannii ICs with the corresponding dominant STs from both the Pasteur and Oxford schemes developed [ 61 ].…”

Section: International Clone (Ic) Definitions Attributes and Propertiesmentioning

confidence: 99%

International Clones of High Risk of Acinetobacter Baumannii—Definitions, History, Properties and Perspectives

Shelenkov,

Akimkin,

Mikhaylova

2023

Microorganisms

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Acinetobacter baumannii is a Gram-negative coccobacillus with exceptional survival skills in an unfavorable environment and the ability to rapidly acquire antibiotic resistance, making it one of the most successful hospital pathogens worldwide, representing a serious threat to public health. The global dissemination of A. baumannii is driven by several lineages named ‘international clones of high risk’ (ICs), two of which were first revealed in the 1970s. Epidemiological surveillance is a crucial tool for controlling the spread of this pathogen, which currently increasingly involves whole genome sequencing. However, the assignment of a particular A. baumannii isolate to some IC based on its genomic sequence is not always straightforward and requires some computational skills from researchers, while the definitions found in the literature are sometimes controversial. In this review, we will focus on A. baumannii typing tools suitable for IC determination, provide data to easily determine IC assignment based on MLST sequence type (ST) and intrinsic blaOXA-51-like gene variants, discuss the history and current spread data of nine known ICs, IC1-IC9, and investigate the representation of ICs in public databases. MLST and cgMLST profiles, as well as OXA-51-like presence data are provided for all isolates available in GenBank. The possible emergence of a novel A. baumannii international clone, IC10, will be discussed.

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“…The highly variable genome empowers MDR P. aeruginosa to survive and disseminate successfully by clonal expansion in addition to the acquisition of various resistance genes [37]. Therefore, the genetic diversity of P. aeruginosa helped the global dispersion of high-risk clones, such as ST235, ST111, ST233, ST244, ST357, ST308, ST175, ST277, ST654 and ST298 [38,39]. Most of the high-risk clones have metallo β-lactamase (MBL) carbapenemase production; however, ST111 and ST235 have multiple carbapenemase genes besides MBL [40].…”

Section: Introductionmentioning

confidence: 99%

Molecular Analysis of Carbapenem and Aminoglycoside Resistance Genes in Carbapenem-Resistant Pseudomonas aeruginosa Clinical Strains: A Challenge for Tertiary Care Hospitals

Gondal,

Choudhry,

Niaz

et al. 2024

Antibiotics

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Carbapenem-resistant Pseudomonas aeruginosa (P. aeruginosa) strains have become a global threat due to their remarkable capability to survive and disseminate successfully by the acquisition of resistance genes. As a result, the treatment strategies have been severely compromised. Due to the insufficient available data regarding P. aeruginosa resistance from Pakistan, we aimed to investigate the resistance mechanisms of 249 P. aeruginosa strains by antimicrobial susceptibility testing, polymerase chain reaction for the detection of carbapenemases, aminoglycoside resistance genes, extended-spectrum beta-lactamases (ESBLs), sequence typing and plasmid typing. Furthermore, we tested silver nanoparticles (AgNPs) to evaluate their in vitro sensitivity against antimicrobial-resistant P. aeruginosa strains. We observed higher resistance against antimicrobials in the general surgery ward, general medicine ward and wound samples. Phenotypic carbapenemase-producer strains comprised 80.7% (201/249) with 89.0% (179/201) demonstrating genes encoding carbapenemases: blaNDM-1 (32.96%), blaOXA48 (37.43%), blaIMP (7.26%), blaVIM (5.03%), blaKPC-2 (1.12%), blaNDM-1/blaOXA48 (13.97%), blaOXA-48/blaVIM (1.68%) and blaVIM/blaIMP (0.56%). Aminoglycoside-modifying enzyme genes and 16S rRNA methylase variants were detected in 43.8% (109/249) strains: aac(6′)-lb (12.8%), aac(3)-lla (12.0%), rmtB (21.1%), rmtC (11.0%), armA (12.8%), rmtD (4.6%), rmtF (6.4%), rmtB/aac(3)-lla (8.2%), rmtB/aac(6′)-lla (7.3%) and rmtB/armA (3.6%). In total, 43.0% (77/179) of the strains coharbored carbapenemases and aminoglycoside resistance genes with 83.1% resistant to at least 1 agent in 3 or more classes and 16.9% resistant to every class of antimicrobials tested. Thirteen sequence types (STs) were identified: ST235, ST277, ST234, ST170, ST381, ST175, ST1455, ST1963, ST313, ST207, ST664, ST357 and ST348. Plasmid replicon types IncFI, IncFII, IncA/C, IncL/M, IncN, IncX, IncR and IncFIIK and MOB types F11, F12, H121, P131 and P3 were detected. Meropenem/AgNPs and Amikacin/AgNPs showed enhanced antibacterial activity. We reported the coexistence of carbapenemases and aminoglycoside resistance genes among carbapenem-resistant P. aeruginosa with diverse clonal lineages from Pakistan. Furthermore, we highlighted AgNP’s potential role in handling future antimicrobial resistance concerns.

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New Variants of Pseudomonas aeruginosa High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital

Cited by 6 publications

References 54 publications

Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China

Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China

International Clones of High Risk of Acinetobacter Baumannii—Definitions, History, Properties and Perspectives

Molecular Analysis of Carbapenem and Aminoglycoside Resistance Genes in Carbapenem-Resistant Pseudomonas aeruginosa Clinical Strains: A Challenge for Tertiary Care Hospitals

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