Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model

Mq, Xie; Zc, Chen; Zhang, P; Hj, Huang; Tt, Wang; Ding, Yu; Ss, Qi; Zhang, C; Sx, Chen; Zhou, Peng; Cc, Shao; Liao, Min; Cy, Sun

doi:10.1016/j.neuroscience.2017.03.045

Cited by 16 publications

(51 citation statements)

References 69 publications

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“…The authors showed a significant increase in the SVZ‐derived NSCs of the third ventricle (3V) and Aq‐surrounding regions. Hence, the SN newborn DAergic neurons were mainly derived from the migration and differentiation of the NSCs in the SVZ of 3V‐ and Aq‐adjacent regions (Xie et al, ), thus supporting our indication that quiescent neuroprogenitors of mNSCs can be activated not only in vitro (L'Episcopo et al, ), but more importantly, in vivo , upon SNpc‐DAergic lesions with PD neurotoxins (L'Episcopo et al, ).…”

Section: Therapeutic Modulation Of Wnt/β‐catenin Signallingsupporting

confidence: 84%

“…Otherwise “dormant” NSC subpopulations can be activated under specific CNS injuries and via cell‐specific signalling mechanisms (Llorenz‐Bobadilla et al, ). After pathological stimulation, neuroprogenitors can be activated in regions otherwise considered to be non‐neurogenic, such as the Str (Bedard, Cossette, Levesque, & Parent, ; Dayer, Cleaver, Abouantoun, & Cameron, ; Inta, Cameron, & Gass, ; Luzzati et al, ; Nato et al, ), while the presence of neurogenesis in the SN still remains a matter of debate (Arzate, Guerra‐Crespob, & Covarrubiasa, ; Barker, Götz, & Parmar, ; Farzanehfar, ; Klaissle et al, ; L'Episcopo et al, ; Lie et al, ; Xie et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Evidence is available that quiescent neuroprogenitors reside in the tegmental aqueduct periventricular region (Aq‐PVRs), which is close to the SNpc and harbors clonogenic NSCs endowed with DAergic potential (Hermann et al, , ; Hermann & Storch, ; L'Episcopo et al, ). Additionally, adult Aq‐PVR NSCs can be activated and induced to differentiate into DAergic neurons, both in vitro and after PD injury in vivo (Hermann et al, , ; Hermann & Stork, ; L'Episcopo et al, , ; L'Episcopo, Tirolo, Peruzzotti‐Jametti, et al, ; Xie et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…SVZ-migrating doublecortin-positive (DCX + ) neuroblasts in red, and dividing, bromodeoxyuridine-positive (BrdU + ) NSCs in green, are seen forming chains traveling along the RMS to the OB where they become granular and periglomerular interneurons involved in odor discrimination. Magnifications of DCX + / BrdU + NSCs are shown in the boxed areas can be activated and induced to differentiate into DAergic neurons, both in vitro and after PD injury in vivo (Hermann et al, 2006(Hermann et al, , 2009Hermann & Stork, 2008;L'Episcopo et al, 2011aL'Episcopo et al, , 2014aL'Episcopo, Tirolo, Peruzzotti-Jametti, et al, 2018a;Xie et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Parkinson's disease, aging and adult neurogenesis: Wnt/β‐catenin signalling as the key to unlock the mystery of endogenous brain repair

et al. 2020

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A common hallmark of age‐dependent neurodegenerative diseases is an impairment of adult neurogenesis. Wingless‐type mouse mammary tumor virus integration site (Wnt)/β‐catenin (WβC) signalling is a vital pathway for dopaminergic (DAergic) neurogenesis and an essential signalling system during embryonic development and aging, the most critical risk factor for Parkinson's disease (PD). To date, there is no known cause or cure for PD. Here we focus on the potential to reawaken the impaired neurogenic niches to rejuvenate and repair the aged PD brain. Specifically, we highlight WβC‐signalling in the plasticity of the subventricular zone (SVZ), the largest germinal region in the mature brain innervated by nigrostriatal DAergic terminals, and the mesencephalic aqueduct‐periventricular region (Aq‐PVR) Wnt‐sensitive niche, which is in proximity to the SNpc and harbors neural stem progenitor cells (NSCs) with DAergic potential. The hallmark of the WβC pathway is the cytosolic accumulation of β‐catenin, which enters the nucleus and associates with T cell factor/lymphoid enhancer binding factor (TCF/LEF) transcription factors, leading to the transcription of Wnt target genes. Here, we underscore the dynamic interplay between DAergic innervation and astroglial‐derived factors regulating WβC‐dependent transcription of key genes orchestrating NSC proliferation, survival, migration and differentiation. Aging, inflammation and oxidative stress synergize with neurotoxin exposure in “turning off” the WβC neurogenic switch via down‐regulation of the nuclear factor erythroid‐2‐related factor 2/Wnt‐regulated signalosome, a key player in the maintenance of antioxidant self‐defense mechanisms and NSC homeostasis. Harnessing WβC‐signalling in the aged PD brain can thus restore neurogenesis, rejuvenate the microenvironment, and promote neurorescue and regeneration.

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Section: Therapeutic Modulation Of Wnt/β‐catenin Signallingsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Parkinson's disease, aging and adult neurogenesis: Wnt/β‐catenin signalling as the key to unlock the mystery of endogenous brain repair

et al. 2020

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“…In this study, all reagents, materials, and chemicals were analytical grade and highest purity, including human neuroblastoma SH-SY5Y cell lines (SCSP-5014, Chinese Academy of Sciences, China); Dulbecco's modified Eagle's medium (DMEM, 11965092), Neurobasal (21103049), B27 (17504044), fetal bovine serum (FBS), glutamax (35050061), trypsin-EDTA (25300062) and sodium pyruvate (11360070; Gibco Invitrogen, MA, USA); APα (3653) and Bic (485494; Tocris, USA); Nifedipine (ab120135, Abcam, Burlingame, CA, USA); RA (R2500) and 6-OHDA (H4381; Sigma-Aldrich, St. Louis, MI, USA); penicillin and streptomycin (Solarbio, Beijing, China); bovine serum albumin (BSA) and BrdU (Bio-Sharp, China); DMSO (ST038), EGTA (ST068), phenylmethanesulfonyl fluoride (PMSF, ST506), TritonX-100, cytosolic and nuclear extraction kit (P0028; Beyotime, China). The concentration of key chemicals was chosen according to the relevant references (Liu et al, 2002;Miloso et al, 2004;Wang et al, , 2010Gilany et al, 2008;Wang and Brinton, 2008;Fernandes et al, 2017;Xie et al, 2017;Taleb et al, 2018). All experiments should comply with the standard biosecurity and safety procedures from the ethical committee of Wenzhou Medical University according to the National Institute of Health Guide for Care and Use of Laboratory Animals (NIH Publications No.…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

RETRACTED: Allopregnanolone Modulates GABAAR-Dependent CaMKIIδ3 and BDNF to Protect SH-SY5Y Cells Against 6-OHDA-Induced Damage

Wang

Bian

et al. 2020

Front. Cell. Neurosci.

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Allopregnanolone (APα), as a functional neurosteroid, exhibits the neuroprotective effect on neurodegenerative diseases such as Parkinson's disease (PD) through γ-aminobutyric acid A receptor (GABAAR), but it has not been completely understood about its molecular mechanisms. In order to investigate the neuroprotective effect of APα, as well as to clarify its possible molecular mechanisms, SH-SY5Y neuronal cell lines were incubated with 6-hydroxydopamine (6-OHDA), which has been widely used as an in vitro model for PD, along with APα alone or in combination with GABAAR antagonist (bicuculline, Bic), intracellular Ca 2+ chelator (EGTA) and voltage-gated L-type Ca 2+ channel blocker (Nifedipine). The viability, proliferation, and differentiation of SH-SY5Y cells, the expression levels of calmodulin (CaM), Ca 2+ /calmodulin-dependent protein kinase II δ3 (CaMKIIδ3), cyclin-dependent kinase-1 (CDK1) and brain-derived neurotrophic factor (BDNF), as well as the interaction between CaMKIIδ3 and CDK1 or BDNF, were detected by morphological and molecular biological methodology. Our results found that the cell viability and the number of tyrosine hydroxylase (TH), bromodeoxyuridine (BrdU) and TH/BrdU-positive cells in 6-OHDA-treated SH-SY5Y cells were significantly decreased with the concomitant reduction in the expression levels of aforementioned proteins, which were ameliorated following APα administration. In addition, Bic could further increase the number of TH or BrdU-positive cells as well as the expression levels of aforementioned proteins except for TH/BrdU-double positive cells, while EGTA and Nifedipine could attenuate the expression levels of CaM, CaMKIIδ3 and BDNF. Moreover, there existed a direct interaction between CaMKIIδ3 and CDK1 or

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Ultrasound‐triggered effects of the microbubbles coupled to GDNF‐ and Nurr1‐loaded PEGylated liposomes in a rat model of Parkinson's disease

Yue

Gao

Wang³

et al. 2018

J of Cellular Biochemistry

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The purpose of this study was to investigate ultrasound-triggered effects of the glial cell line-derived neurotrophic factor (GDNF) + nuclear receptor-related factor 1 (Nurr1)-polyethylene glycol (PEG)ylated liposomes-coupled microbubbles (PLs-GDNF + Nurr1-MBs) on behavioral impairment and neuron loss in a rat model of Parkinson's disease (PD). The unloaded PEGylated liposomes-coupled microbubbles (PLs-MBs) were characterized for zeta potential, particle size, and concentration. 6-hydroxydopamine (6-OHDA) was used to establish the PD rat model. Rotational, climbing pole, and suspension tests were used to detect behavioral impairment. The immunohistochemical staining of tyrosine hydroxylase (TH) and dopamine transporter (DAT) was used to assess the neuron loss. Western blot and quantitative real-time PCR (qRT-PCR) analysis were used to measure the expression levels of GDNF and Nurr1. The particle size of PLs-MBs was gradually increased, while the concentration and absolute zeta potential were gradually decreased as the time prolongs. 6-OHDA increased amphetamine-induced rotations and loss of dopaminergic neurons as compared to sham group. Interestingly, PLs-GDNF-MBs or PLs-Nurr1-MBs decreased rotations and increased the TH and DAT immunoreactivity. Combined of both genes resulted in a robust reduction in the rotations and a greater increase of the dopaminergic neurons. The delivery of PLs-GDNF + Nurr1-MBs into the brains using magnetic resonance imaging (MRI)-guided focused ultrasound may be more efficacious for the treatment of PD than the single treatment.

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Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model

Cited by 16 publications

References 69 publications

Parkinson's disease, aging and adult neurogenesis: Wnt/β‐catenin signalling as the key to unlock the mystery of endogenous brain repair

Parkinson's disease, aging and adult neurogenesis: Wnt/β‐catenin signalling as the key to unlock the mystery of endogenous brain repair

RETRACTED: Allopregnanolone Modulates GABAAR-Dependent CaMKIIδ3 and BDNF to Protect SH-SY5Y Cells Against 6-OHDA-Induced Damage

Ultrasound‐triggered effects of the microbubbles coupled to GDNF‐ and Nurr1‐loaded PEGylated liposomes in a rat model of Parkinson's disease

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