Next-generation sequencing analysis of semen microbiome taxonomy in men with nonobstructive azoospermia vs. fertile controls: a pilot study

Campbell, Katherine; Arbeláez, María Camila Suárez; Ghomeshi, Armin; Ibrahim, Elsayed Mohammed; Roy, Sabita; Singh, Preetvanti; Khodamoradi, Kajal; Miller, Aaron; Lundy, Scott; Ramasamy, Ranjith

doi:10.1016/j.xfss.2023.06.001

Cited by 3 publications

(4 citation statements)

References 34 publications

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“…As metagenomics technology, this approach provides a massive molecular characterization and quantification without a previous selection of species and offers wide possibilities of subsequent bioinformatics analyses, like taxonomic diversity studies (alpha and beta diversity) or clustering. The characterization of semen microbiota through 16S rRNA sequencing has broadened the range of bacterial elements discovered and shed light on aspects like their potential association with health and fertility [ 9 , 25 , 26 , 34 , 79 , 87 , 89 ] or their possible inter-relations [ 22 , 57 ]. In addition, other NGS strategies have been applied in the study of seminal microbiota.…”

Section: Technical Considerations In the Study Of The Seminal Microbiomementioning

confidence: 99%

“…Low rates of normal morphology in sperm have been observed in samples with the abundance of Staphylococcus aureus, Ureaplasma urealyiticum, HCMV, and human polyomavirus 2 (JCPyV) [34,76,[83][84][85], and low sperm counts have been associated with the presence of Staphylococcus, Haemophilus, Klebsiella, Chlamydia trachomatis, and ZIKV [25,32,56,74,76,82]. Patients with azoospermia have shown more abundance of Mycoplasma and Ureaplasma [27], as well as an increase in Bacteroidetes, Firmicutes [86], Proteobacteria, and Actinobacteria [87]. Contrarily, Lactobacillus tends to predominate in the semen microbiota of healthy and fertile men with good quality semen parameters [22,31,32,57,78,88].…”

Section: The Semen Microbiome In Fertility Disordersmentioning

confidence: 99%

See 1 more Smart Citation

Unraveling the Intricacies of the Seminal Microbiome and Its Impact on Human Fertility

Corral-Vazquez,

Blanco,

Sarrate

et al. 2024

Biology

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Although the microbial communities from seminal fluid were an unexplored field some decades ago, their characteristics and potential roles are gradually coming to light. Therefore, a complex and specific microbiome population with commensal niches and fluctuating species has started to be revealed. In fact, certain clusters of bacteria have been associated with fertility and health, while the outgrowth of several species is potentially correlated with infertility indicators. This constitutes a compelling reason for outlining the external elements that may induce changes in the seminal microbiome composition, like lifestyle factors, gut microbiota, pathologies, prebiotics, and probiotics. In this review, we summarize the main findings about seminal microbiome, its origins and composition, its relationship with fertility, health, and influence factors, while reminding readers of the limitations and advantages introduced from technical variabilities during the experimental procedures.

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Section: Technical Considerations In the Study Of The Seminal Microbiomementioning

confidence: 99%

Section: The Semen Microbiome In Fertility Disordersmentioning

confidence: 99%

Unraveling the Intricacies of the Seminal Microbiome and Its Impact on Human Fertility

Corral-Vazquez,

Blanco,

Sarrate

et al. 2024

Biology

View full text Add to dashboard Cite

show abstract

“…They found that alpha diversity was similar between the groups. At genera level, Escherichia, Shigella, Sneathia and Raoutella differed significantly between groups [64]. Chen et al also investigated the microbiomes of men with NOA (n = 30) and normal controls (n = 30) via NGS of the V3-V4 16S RNA hypervariable region [9].…”

Section: Molina Et Al Assessed the V3-4 Hypervariable Region Of 16s R...mentioning

confidence: 99%

Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders

Davies,

Minhas,

Jayasena

2023

Medicina

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Mean sperm counts are declining at an accelerated rate and infertility is increasingly becoming a public health concern. It is now understood that human semen, previously considered to be sterile, harbours its own specific microbiome. Via activated leucocytes and the generation of reactive oxygen species, bacteria have the capability of evoking an immune response which may lead to sperm damage. Men with infertility have higher rates of both reactive oxygen species and sperm DNA damage. Due to the lack of sensitivity of routine culture and PCR-based methods, next-generation sequencing technology is being employed to characterise the seminal microbiome. There is a mounting body of studies that share a number of similarities but also a great range of conflicting findings. A lack of stringent decontamination procedures, small sample sizes and heterogeneity in other aspects of methodology makes it difficult to draw firm conclusions from these studies. However, various themes have emerged and evidence of highly conserved clusters of common bacteria can be seen. Depletion or over-representation of specific bacteria may be associated with aberrations in traditional and functional seminal parameters. Currently, the evidence is too limited to inform clinical practice and larger studies are needed.

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“…Their findings underscore differences in alpha and beta diversity among infertile men compared with healthy controls, in addition to a direct association between genus Pseudomonas and total motile sperm count. Another study with a similar sample size found that men with non-obstructive azoospermia compared to healthy controls, demonstrate changes in semen microbiome beta diversity and associated taxonomic differences 6 .…”

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confidence: 90%

Semen microbiota are dramatically altered in men with abnormal sperm parameters

Osadchiy,

Belarmino,

Kianian

et al. 2024

Sci Rep

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There has recently been an explosion of studies implicating the human microbiome in playing a critical role in many disease and wellness states. The etiology of abnormal semen analysis (SA) parameters is not identified in 30% of cases; investigations involving the semen microbiome may bridge this gap. Here, we explore the relationship between the semen microbiome and alterations of sperm parameters. We recruited men presenting for fertility evaluation or vasectomy consultation with proven biological paternity. SA and next generation sequencing was performed. Differential abundance testing using Analysis of composition of Microbiota with Bias Correction (ANCOM-BC) was performed along with canonical correlational analysis for microbial community profiling. Men with abnormal (N = 27) sperm motility showed a higher abundance of Lactobacillus iners compared to those with normal (N = 46) sperm motility (mean proportion 9.4% versus 2.6%, p = 0.046). This relationship persisted on canonical correlational analysis (r = 0.392, p = 0.011). Men with abnormal sperm concentration (N = 20) showed a higher abundance of Pseudomonas stutzeri (2.1% versus 1.0%, p = 0.024) and Pseudomonas fluorescens (0.9% versus 0.7%, p = 0.010), but a lower abundance of Pseudomonas putida (0.5% versus 0.8%, p = 0.020), compared to those with normal sperm concentration (N = 53). Major limitations are related to study design (cross-sectional, observational). Our results suggest that a small group of microorganisms may play a critical role in observed perturbations of SA parameters. Some of these microbes, most notably Lactobacillus iners, have been described extensively within other, fertility-related, contexts, whereas for others, this is the first report where they have potentially been implicated. Advances in our understanding of the semen microbiome may contribute to potentially new therapeutic avenues for correcting impairments in sperm parameters and improving male fertility.

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Next-generation sequencing analysis of semen microbiome taxonomy in men with nonobstructive azoospermia vs. fertile controls: a pilot study

Cited by 3 publications

References 34 publications

Unraveling the Intricacies of the Seminal Microbiome and Its Impact on Human Fertility

Unraveling the Intricacies of the Seminal Microbiome and Its Impact on Human Fertility

Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders

Semen microbiota are dramatically altered in men with abnormal sperm parameters

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