“…Following the allocation of the articles that met the eligibility criteria, we extracted several strong points: - The body mass index (BMI) and obesity influence the chances of implantation and amplify the risk of miscarriage [ 40 , 46 ], also dependent on the couple’s age [ 27 ], cesarean section (C-section) [ 22 ], and ovarian reserve [ 23 ], rather than correlated with previous unsuccessful pregnancies [ 36 ];
- While age exerts a detrimental effect, it is mitigated through SNP-based PGT-A [ 39 ], and the embryos’ morphology possesses a significant threat with greater impact [ 11 , 37 , 47 ], but contradicted on several occasions [ 21 , 29 , 38 ]; Embryo morphokinetic [ 11 , 34 , 37 ] and inner cell mass (ICM) morphology constitute an optimal predictor of sustained implantation [ 48 ];
- Mitochondrial DNA (mtDNA) copy numbers are higher in day 5 blastocysts of older women than day 6 blastocysts, further associated with a low chance of ongoing pregnancies [ 14 , 24 ]; The content of mtDNA is unable to predict the miscarriage risk [ 12 ] and additionally refuted when comparing the outcome differences between them [ 19 ] despite the cryo-storage [ 49 ];
- Despite the sensitivity of platforms, errors still might occur, and their optimization is mandatory; PGT-NGS significantly improves the clinical outcomes in mosaic embryos [ 28 , 41 , 50 ], FAST-SeqS being a reliable and scalable PGT-A method [ 15 ];
- Mosaic embryos have poor reproductive potential but retain the ability to result in live births [ 13 , 33 ], further sustaining that TE biopsy did not add detectable adverse effects [ 42 ] but as a supplement for the management of recurrent implantation failure (RIF) [ 45 ...
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