2021
DOI: 10.1007/s43032-021-00519-0
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Next-Generation Sequencing (NGS)-Based Preimplantation Genetic Testing for Aneuploidy (PGT-A) of Trophectoderm Biopsy for Recurrent Implantation Failure (RIF) Patients: a Retrospective Study

Abstract: Recurrent implantation failure (RIF) is an intrigue condition during in vitro fertilization (IVF) cycles or intracytoplasmic sperm injection (ICSI) treatments. The purpose of this retrospective study is to explore the value of next-generation sequencing (NGS)-based preimplantation genetic testing for aneuploidy (PGT-A) of trophectoderm biopsy in the clinical outcomes for RIF patients with advanced age. A total of 265 RIF patients, who underwent 346 oocyte retrieval cycles and 250 PGT-A cycles, were classified … Show more

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Cited by 27 publications
(30 citation statements)
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“…Illumina twelve with MiSeq [ 11 , 13 , 14 , 16 , 24 , 27 , 29 , 33 , 34 , 38 , 43 , 44 ]; three with HiSeq [ 15 , 28 , 32 ]; two with NextSeq [ 31 , 41 ]; one with sequencing-by-synthesis [ 45 ]; …”
Section: Resultsmentioning
confidence: 99%
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“…Illumina twelve with MiSeq [ 11 , 13 , 14 , 16 , 24 , 27 , 29 , 33 , 34 , 38 , 43 , 44 ]; three with HiSeq [ 15 , 28 , 32 ]; two with NextSeq [ 31 , 41 ]; one with sequencing-by-synthesis [ 45 ]; …”
Section: Resultsmentioning
confidence: 99%
“…Following the allocation of the articles that met the eligibility criteria, we extracted several strong points: The body mass index (BMI) and obesity influence the chances of implantation and amplify the risk of miscarriage [ 40 , 46 ], also dependent on the couple’s age [ 27 ], cesarean section (C-section) [ 22 ], and ovarian reserve [ 23 ], rather than correlated with previous unsuccessful pregnancies [ 36 ]; While age exerts a detrimental effect, it is mitigated through SNP-based PGT-A [ 39 ], and the embryos’ morphology possesses a significant threat with greater impact [ 11 , 37 , 47 ], but contradicted on several occasions [ 21 , 29 , 38 ]; Embryo morphokinetic [ 11 , 34 , 37 ] and inner cell mass (ICM) morphology constitute an optimal predictor of sustained implantation [ 48 ]; Mitochondrial DNA (mtDNA) copy numbers are higher in day 5 blastocysts of older women than day 6 blastocysts, further associated with a low chance of ongoing pregnancies [ 14 , 24 ]; The content of mtDNA is unable to predict the miscarriage risk [ 12 ] and additionally refuted when comparing the outcome differences between them [ 19 ] despite the cryo-storage [ 49 ]; Despite the sensitivity of platforms, errors still might occur, and their optimization is mandatory; PGT-NGS significantly improves the clinical outcomes in mosaic embryos [ 28 , 41 , 50 ], FAST-SeqS being a reliable and scalable PGT-A method [ 15 ]; Mosaic embryos have poor reproductive potential but retain the ability to result in live births [ 13 , 33 ], further sustaining that TE biopsy did not add detectable adverse effects [ 42 ] but as a supplement for the management of recurrent implantation failure (RIF) [ 45 ...…”
Section: Resultsmentioning
confidence: 99%
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“…The assessment of embryo ploidy status allowed for improved assessment of endometrial contribution for RIF. NGS-based embryo PGT-A represents a valuable supplement to the current management of RIF [11]. Our study assessed endometrial receptivity and embryo ploidy status in RIF patients by ERA and PGT-A, respectively, and assessed their reliability.…”
Section: Discussionmentioning
confidence: 99%
“…The current study has a prospective design and aims to help people with advanced age and RIF by determining the effectiveness of next-generation sequencing (NGS) based PGT-Aof blastocysts' trophectoderm biopsy on their clinical outcomes. The study recruited 265 couples who suffered from RIF in previous cycles and underwent 250cycles of PGT-A and 346 cycles of follicle aspiration and they were divided into two groups, those in which the female partner is 38 years old or older, and those in which the females are less than 38 years old (16) . Findings showed a significantly lower aneuploidy rate in the embryos of the younger group compared to the older group (39.9 vs. 68.9 %, P< 0.001).…”
Section: Discussionmentioning
confidence: 99%