NF-κB dependent anti-inflammatory activity of chlorojanerin isolated from Saussurea heteromalla

Saklani, Arvind; Hegde, Bindu; Mishra, Prabha B.; Singh, Ruchi; Mendon, Monica; Chakrabarty, Debarshi; Kamath, Divya; Lobo, Aurelio; Prabhu, K. Narayan; Dagia, Nilesh M.; Padigaru, Muralidhara; Kulkarni-Almeida, Asha

doi:10.1016/j.phymed.2012.05.016

Cited by 18 publications

(7 citation statements)

References 25 publications

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“…1 H NMR (700 MHz, DMSO- d 6 ) δ 6.17 (s, Ha-18), 5.99 (s, Ha-13), 5.90 (s, Hb-18), 5.49 (s, Hb-13), 5.17 (s, Ha-14), 4.99 (m, H-8), 4.85 (s, Hb-14), 4.63 (t, J = 9.9 Hz, H-6), 4.17 (s, H-19), 3.22 (m, H-7), 3.10 (m, H-1), 3.06 (s, Ha-15), 2.96 (s, Hb-15), 2.69 (d, J = 14.3 Hz, Ha-9), 2.27 (d, J = 14.6 Hz, Hb-9), 2.21 (m, H-2). 1 H NMR and 13 C NMR was also reported by Saklani et al [ 15 ].…”

Section: Methodssupporting

confidence: 69%

“…Molecules 2023, 28, 3061 2 of 13 Chlorojanerin (a guaianolide-type sesquiterpene lactone) was previously isolated from several species of the Centaurea genus including Centaurea hermannii [12], Centaurea musimomum [13], Centaurea solstitialis [14], as well as Centaurothamnus maximus [10]. The biological studies of chlorojanerin have revealed that it possesses various effects such as anti-ulcerogenic [14], antiinflammatory [15] and antiviral and antimicrobial activities [16]. Additionally, chlorojanerin has showed promising effectiveness in preventing the growth of various cancer cells [10,17].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the Anticancer Effect of Chlorojanerin Isolated from Centaurothamnus maximus on A549 Lung Cancer Cells

et al. 2023

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The goal of this study was to assess the anticancer efficacy of chlorojanerin against various cancer cells. The effects of chlorojanerin on cell cytotoxicity, cell cycle arrest, and cell apoptosis were examined using MTT assay, propidium iodide staining, and FITC Annexin V assay. RT-PCR was employed to determine the expression levels of apoptosis-related genes. Furthermore, docking simulations were utilized to further elucidate the binding preferences of chlorojanerin with Bcl-2. According to MTT assay, chlorojanerin inhibited the proliferation of all tested cells in a dose-dependent manner with a promising effect against A549 lung cancer cells with an IC50 of 10 µM. Cell growth inhibition by chlorojanerin was linked with G2/M phase cell cycle arrest in A549 treated cells. Flow cytometry analysis indicated that the proliferation inhibition effect of chlorojanerin was associated with apoptosis induction in A549 cells. Remarkably, chlorojanerin altered the expression of many genes involved in apoptosis initiation. Moreover, we determined that chlorojanerin fit into the active site of Bcl-2 according to the molecular docking study. Collectively, our results demonstrate that chlorojanerin mediated an anticancer effect involving cell cycle arrest and apoptotic cell death and, therefore, could potentially serve as a therapeutic agent in lung cancer treatment.

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Section: Methodssupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Assessment of the Anticancer Effect of Chlorojanerin Isolated from Centaurothamnus maximus on A549 Lung Cancer Cells

et al. 2023

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“…Himalaiella heteromalla (D.Don) Raab-Straube ( Asteraceae ), commonly known as Batula, is found in low-temperature regions of Asia, Europe, and North America [ 1 ]. Himalaiella heteromalla is a rich source of chlorojanerin, arctigenin [ 2 ] glycosides, alkaloids, terpenoids, saponins, flavonoids, sesquiterpene lactones, and arctiin [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Antidiarrheal and Cardio-Depressant Effects of Himalaiella heteromalla (D.Don) Raab-Straube: In Vitro, In Vivo, and In Silico Studies

Saqib

Usman

Malik

et al. 2021

Plants

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Himalaiella heteromalla (D.Don) Raab-Straube is a commonly used remedy against various diseases. Crude extract and fractions of H. heteromalla were investigated for a gastrointestinal, bronchodilator, cardiovascular, and anti-inflammatory activities. H. heteromalla crude extract (Hh.Cr) relaxed spontaneous contractions and K+ (80 mM)-induced contraction in jejunum tissue dose-dependently. The relaxation of K+ (80 mM) indicates the presence of Ca++ channel blocking (CCB) effect, which was further confirmed by constructing calcium response curves (CRCs) as they caused rightward parallel shift of CRCs in a manner comparable to verapamil, so the spasmolytic effect of Hh.Cr was due to its CCB activity. Application of Hh.Cr on CCh (1 µM) and K+ (80 mM)-induced contraction in tracheal preparation resulted in complete relaxation, showing its bronchodilator effect mediated through Ca++ channels and cholinergic antagonist activity. Application of Hh.Cr on aortic preparations exhibited vasorelaxant activity through angiotensin and α-adrenergic receptors blockage. It also showed the cardio suppressant effect with negative chronotropic and inotropic response in paired atrium preparation. Similar effects were observed in in vivo models, i.e., decreased propulsive movement, wet feces, and inhibition of edema formation.

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“…NFkB is a transcription factor which is cell specific, has an important role in cell differentiation and regulates specific cellular responses. In the monocyte-macrophage lineage, NFkB is a master transcriptional factor which regulates TNFa and IL-6 secretion (Saklani et al 2012). From the above-mentioned studies, we can deduce that the action of (2 )hinokinin in the inhibition of the cytokines TNFa and IL-6 may be mediated through the NFkB signalling pathway, whereas aristolactam I could be acting through signalling mechanisms independent of NFkB activation.…”

Section: Resultsmentioning

confidence: 99%

Isolation, structural elucidation and anti-inflammatory activity of astragalin, ( − )hinokinin, aristolactam I and aristolochic acids (I & II) fromAristolochia indica

Desai

Jacob

Almeida

et al. 2014

Natural Product Research

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Astragalin was isolated for the first time along with (-)hinokinin, aristolactam I and aristolochic acids (I & II) from the extracts of Aristolochia indica L. using a new, efficient preparative HPLC method. A reversed-phase HPLC method of analysis was developed to analyse the isolated compounds. The crude extracts and the isolated compounds were tested for their anti-inflammatory potential. We report here for the first time the anti-inflammatory effects of (-)hinokinin and aristolactam I against IL-6 (IC50 = 20.5 ± 0.5 and 52 ± 8 μM) and TNFα (IC50 = 77.5 ± 27.5 and 116.8 ± 83.25 μM), respectively. (-)Hinokinin exerted its anti-inflammatory effects via NFκB-dependent mechanism whereas aristolactam I may be effective via a mechanism independent of NFκB.

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NF-κB dependent anti-inflammatory activity of chlorojanerin isolated from Saussurea heteromalla

Cited by 18 publications

References 25 publications

Assessment of the Anticancer Effect of Chlorojanerin Isolated from Centaurothamnus maximus on A549 Lung Cancer Cells

Assessment of the Anticancer Effect of Chlorojanerin Isolated from Centaurothamnus maximus on A549 Lung Cancer Cells

Antidiarrheal and Cardio-Depressant Effects of Himalaiella heteromalla (D.Don) Raab-Straube: In Vitro, In Vivo, and In Silico Studies

Isolation, structural elucidation and anti-inflammatory activity of astragalin, ( − )hinokinin, aristolactam I and aristolochic acids (I & II) fromAristolochia indica

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