NF-κB/Rel-Mediated Regulation of the Neural Fate in Drosophila

Ayyar, Savita; Pistillo, Daniela; Calleja, M.; Brookfield, Anna; Gittins, Kelly; Goldstone, Claire; Simpson, Patricia

doi:10.1371/journal.pone.0001178

Cited by 32 publications

(34 citation statements)

References 42 publications

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“…1D) sc activation and bristle patterning and they are expressed for a considerable period before ac-sc gene products are detected (Calleja et al, 2000;Klein, 2001;Calleja et al, 2002;Cavodeassi et al, 2002;Ghazi, 2003;Ikmi et al, 2008). Furthermore, although activation of ac-sc in proneural clusters by Pnr and Iro-C dramatically increases transcription at these sites, the ac-sc genes are also expressed at low levels over the entire disc epithelium, presumably through activity of the basal promoters (Martinez and Modolell, 1991;Ayyar et al, 2007). Indeed maintenance of proneural genes in an active state of basal transcription is a general feature of neuroepithelia (Bertrand et al, 2002).…”

Section: Research Articlementioning

confidence: 99%

“…Other factors must be involved on the lateral notum. One possibility is NFk-B/Rel, a factor that is required for functioning of the SOPE and singling out of precursors, and that also indirectly affects the 331 RESEARCH ARTICLE Phosphorylation of Scute by Sgg stability of sc transcripts (Culi and Modolell, 1998;Ayyar et al, 2007). Another event that coincides with the accumulation of acsc products at mid third larval instar is a small peak of 20H-ecdysteroid (not associated with a moult) (Berreur et al, 1979;Riddiford, 1993).…”

Section: Research Articlementioning

confidence: 99%

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The kinase Sgg modulates temporal development of macrochaetes inDrosophilaby phosphorylation of Scute and Pannier

2012

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SUMMARYEvolution of novel structures is often made possible by changes in the timing or spatial expression of genes regulating development. Macrochaetes, large sensory bristles arranged into species-specific stereotypical patterns, are an evolutionary novelty of cyclorraphous flies and are associated with changes in both the temporal and spatial expression of the proneural genes achaete (ac) and scute (sc). Changes in spatial expression are associated with the evolution of cis-regulatory sequences, but it is not known how temporal regulation is achieved. One factor required for ac-sc expression, the expression of which coincides temporally with that of ac-sc in the notum, is Wingless (Wg; also known as Wnt). Wingless downregulates the activity of the serine/threonine kinase Shaggy (Sgg; also known as GSK-3). We demonstrate that Scute is phosphorylated by Sgg on a serine residue and that mutation of this residue results in a form of Sc with heightened proneural activity that can rescue the loss of bristles characteristic of wg mutants. We suggest that the phosphorylated form of Sc has reduced transcriptional activity such that sc is unable to autoregulate, an essential function for the segregation of bristle precursors. Sgg also phosphorylates Pannier, a transcriptional activator of ac-sc, the activity of which is similarly dampened when in the phosphorylated state. Furthermore, we show that Wg signalling does not act directly via a cis-regulatory element of the ac-sc genes. We suggest that temporal control of ac-sc activity in cyclorraphous flies is likely to be regulated by permissive factors and might therefore not be encoded at the level of ac-sc gene sequences.

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Section: Research Articlementioning

confidence: 99%

Section: Research Articlementioning

confidence: 99%

The kinase Sgg modulates temporal development of macrochaetes inDrosophilaby phosphorylation of Scute and Pannier

2012

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“…Cleaved Spätzle acts as a ligand for the receptor Toll and stimulates the assembly of the receptor-adaptor complex (7)(8)(9). These signaling events target the transcription factors Dorsal and DIF, as well as the inhibitor Cactus, which are proteins related to those within the mammalian NF-κB/IκB complex (10)(11)(12)(13). Toll signaling increases the nuclear localization of Dorsal and DIF, which in turn bind to κB motifs on the promoters of antimicrobial peptide genes (3,13).…”

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confidence: 99%

Heterodimers of NF-κB transcription factors DIF and Relish regulate antimicrobial peptide genes in Drosophila

Tanji

Yun

2010

Proc. Natl. Acad. Sci. U.S.A.

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The innate immune response in Drosophila involves the inducible expression of antimicrobial peptide genes mediated by the Toll and IMD signaling pathways. Dorsal and DIF act downstream of Toll, whereas Relish acts downstream of IMD to regulate target gene expression. Dorsal, DIF, and Relish are NF-κB-related transcription factors and function as obligate dimers, but it is not clear how the various dimer combinations contribute to the innate immune response. We systematically examined the dimerization tendency of these proteins through the use of transgenic assays. The results show that all combinations of homo-and heterodimers are formed, but with varying degrees of efficiency. The formation of the DIFRelish heterodimer is particularly interesting because it may mediate signaling for the seemingly independent Toll and IMD pathways. By incorporating a flexible peptide linker, we specifically tested the functions of the DIF^Relish (a^sign represents the peptide linker) linked heterodimer. Our results demonstrate that the linked heterodimer can activate target genes of both the Toll and IMD pathways. The DIF and Relish complex is detectable in whole animal extracts, suggesting that this heterodimer may function in vivo to increase the spectrum and level of antimicrobial peptide production in response to different infections.IMD | innate immunity | Toll

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“…Транскрипция AS-C инициируется транскрипционны-ми факторами семейства NF-kB/Rel (Ayyar et al, 2007(Ayyar et al, , 2010, белками комплексов iroquois (Araucan, Caupolican и Mirror) (Leyns et al, 1996) и Bar (BarH1 и BarH2) (Sato et al, 1999), а также дуэтом Chip/Pnr в составе гетеромера (de Navascués, Modolell, 2007;Bronstein et al, 2010).…”

Section: системная биология и моделированиеunclassified

Analysis of the Neurogenesis: Prepattern gene network сontrolling the first stage in Drosophila melanogaster bristle pattern development

Furman¹,

Бухарина²

2016

Vestn. VOGiS

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The external insect chitinous skeleton is unable to respond to stimuli; the external signals are received by specialized receptors. Drosophila perceives the tactile stimuli by its external sensory organs, the microchaetes and macrochaetes residing on the head and back (notum). The microchaetes (hairs) are numerous and arranged in perfect rows along the body. The macrochaetes (bristles) are rather few and are strictly positioned on the head and notum, being referred to as bristle pattern. Bristles act as mechanoreceptors, providing balance for flying dro sophila. The proper bristle pattern of an adult fly develops through several stages. Its basic stage is formation of prepattern for the future bristles, repre sented by proneural clusters. The proneural clusters separate from the ectodermal cells in imaginal discs in the third instar larvae and early prepupae. They are induced by prepattern factors, identified with the transcription factors driving expression of their target genes in certain disc regions. Reconstruction of the gene network controlling prepattern development and its analysis are for the first time described as well as the principles underlying arrangement and function of this network. The hierarchical structure of the net work, its key components, and regulatory circuits are identified. The network comprises 80 entities interconnected via 109 regulatory interactions. The key objects of the network, displaying the greatest connectivity with its other components, are the ASC proneural proteins encoded by the achaete and scute genes, and the proteins Decapentaplegic (Dpp) and Wingless (Wg). The structure of the network is hierarchical and has at least three control levels. The network acts as a gene ensemble owing to coordinated functioning of the regulatory circuits Хитиновый покров насекомых не способен реагировать на раз дражители, и приемниками сигналов из окружающей среды для них служат специализированные рецепторы. Дрозофила воспринимает тактильные стимулы посредством внешних сен сорных органов -микро и макрохет, расположенных на голове и спинке (нотуме). Микрохеты многочисленны и образуют пра вильные ряды, ориентированные вдоль тела. Число макрохет относительно невелико, и их расположение на голове и нотуме строго определено. Макрохеты выполняют функцию механо рецепторов, обеспечивающих мухе сохранение равновесия в полете. Набор макрохет называют щетиночным узором. Полно ценный щетиночный узор взрослой Drosophila melanogaster скла дывается в результате многоступенчатого процесса. Осно во по ла гающая его стадия состоит в создании прообраза расположения будущих макрохет -препаттерна (предструктуры), представлен ного пронейральными кластерами. Пронейральные кластеры обособляются из массы клеток крыловых имагинальных дисков на стадии личинки третьего возраста и ранней предкуколки под действием факторов предструктуры, отождествляемых с транс крипционными факторами, направленно регулирующими экспрессию своих геновмишеней в соответствующих районах. В статье впервые приведены результаты реконструкции...

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NF-κB/Rel-Mediated Regulation of the Neural Fate in Drosophila

Cited by 32 publications

References 42 publications

The kinase Sgg modulates temporal development of macrochaetes inDrosophilaby phosphorylation of Scute and Pannier

The kinase Sgg modulates temporal development of macrochaetes inDrosophilaby phosphorylation of Scute and Pannier

Heterodimers of NF-κB transcription factors DIF and Relish regulate antimicrobial peptide genes in Drosophila

Analysis of the Neurogenesis: Prepattern gene network сontrolling the first stage in Drosophila melanogaster bristle pattern development

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