NFIB functions as an oncogene in estrogen receptor-positive breast cancer and is regulated by miR-205-5p

Chen, Hanxiao; Yu, Changqian; Shen, Lu; Wu, Yanqian; Wu, Deqi; Wang, Zhen; Song, Guangzhong; Chen, Linjie; Hong, Yeting

doi:10.1016/j.prp.2020.153236

Cited by 8 publications

(3 citation statements)

References 35 publications

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“…Besides that, recent studies also suggested that NFIB is involved in the tumorigenesis of many types of cancers by serving as an oncogene (Liu et al 2019b;Kashiwagi et al 2011;Dooley et al 2011). In BC, NFIB was also verified to function as a cancer promoter to induce BC cell proliferate and inhibit apoptosis (Moon et al 2011;Liu et al 2019c;Chen et al 2020). In the present work, we verified the binding between NFIB and miR-590-5p; moreover, circ_0069718 acted as a sponge for miR-590-5p to elevate NFIB expression.…”

Section: Discussionmentioning

confidence: 92%

Circ_0069718 promotes the progression of breast cancer by up-regulating NFIB through sequestering miR-590-5p

Zhao

Shi

2021

Mamm Genome

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Researches indicate that circular RNAs are dysregulated in breast cancer (BC) and play a critical role in regulating the malignant phenotype of cancer cells. Herein, the goal of this work was to investigate the role and mechanism of circ_0069718 in BC development. Levels of genes and proteins were detected by quantitative real-time polymerase chain reaction and western blot. In vitro experiments were performed using cell counting kit-8 assay, colony formation assay, EdU (5-ethynyl-2ʹ-deoxyuridine) assay, flow cytometry, western blot, and transwell assay, respectively. The dual-luciferase reporter and RNA immunoprecipitation assays were used to identify the target relationship between miR-590-5p and circ_0069718 or nuclear factor I/B (NFIB). In vivo experiments were conducted using Xenograft model in mice. Circ_0069718 was up-regulated in BC tissues and cells. Knockdown of circ_0069718 suppressed BC cell apoptosis, migration, and invasion in vitro effectively. Mechanistically, circ_0069718 directly targeted miR-590-5p to up-regulate its target NFIB. Rescue experiments showed that miR-590-5p inhibition reversed the inhibitory effects of circ_0069718 knockdown on BC cell-aggressive oncogenic phenotypes; moreover, miR-590-5p re-expression restrained BC cell proliferation and mobility, which were abolished by NFIB up-regulation. Besides that, circ_0069718 silencing hindered tumor growth via miR-590-5p/NFIB axis in vivo. Circ_0069718 promotes BC progression by up-regulating NFIB through sequestering miR-590-5p, suggesting a potential therapeutic strategy in BC.

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Section: Discussionmentioning

confidence: 92%

Circ_0069718 promotes the progression of breast cancer by up-regulating NFIB through sequestering miR-590-5p

Zhao

Shi

2021

Mamm Genome

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“…As has been reported, miR-205-5p is involved in the development of various types of cancers (31)(32)(33). For example, overexpression of miR-205-5p was detected in patients with non-small cell lung cancer in comparison with the contrast groups (34), miR-205-5p was signi cantly downregulated in estrogen receptor-positive breast cancer by targeting to NFIB (35). Related studies have shown the regulatory role of miR-205-5p in epithelial-mesenchymal transition by targeting PTEN via PI3K/AKT signaling pathway in cisplatin-resistant nasopharyngeal carcinoma cells (36), miR-205-5p inhibits PTK7, thereby involved in the proliferation, migration and invasion of CRC (37).…”

Section: Discussionmentioning

confidence: 57%

Tumor-exosomal miR-205-5p as a diagnostic biomarker for colorectal cancer

Zhao,

Liu

et al. 2024

Preprint

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Background: Tumor-exosomal miRNA play crucial roles in cancer diagnosis. The current reports aimed to found an exosomal miRNA for colorectal cancer(CRC) with non-invasiveness, sensitivity and specificity. Patients and methods: The exosome was extracted from CRC patients and healthy donors using ultracentrifugation to verify by TEM, qNano and western blot. Differential expression level and clinical characterization of miR-205-5p were analyzed in colorectal cancer via TCGA. Real Time Quantitative PCR was employed to determine the different expression levels of exosomal miRNAs in 157 primary CRC patients and 135 healthy donors. Predictions were made concerning target genes to determine the direction for further exploring the etiopathogenesis of the disease by means of bioinformatics. Results: The expression of miR-205-5p demonstrated a substantial downregulation in colorectal cancer compared to healthy donors, as determined through analysis of the TCGA database. We conducted a prediction and analysis of the functional enrichment of downstream target genes regulated by miR-205-5p. Reduced level of exosomal miR-205-5p in serum from CRC patients was found compared with healthy controls (p<0.0001, respectively) and benign disease (p<0.0001, respectively). The levels of expression of exosomal miR-205-5p were substantially lower in early stage CRC patients than in the contrast groups (p<0.0001, respectively). The expression levels of exosomal miR-205-5p saw significant increase postoperatively (p=0.0053, respectively). Conclusions: The present study demonstrated that serum exosomal miR-205-5p may be a diagnostic biomarker for colorectal cancer.

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“…MiR-205 has been also demonstrated to negatively regulate the oncogene Nuclear factor I/B (NFIB) in ER+ breast cancer [20]. Chen H et al demonstrated that Nuclear factor I/B (NFIB) was upregulated in ER+ breast cancer versus normal tissue and that NFIB promoted MCF-7 cell cycle progression and proliferation in in vitro experiments.…”

Section: Role Of Mir-205 In Cell Growth Proliferation and Responsiveness To Therapymentioning

confidence: 99%

miR-205 in Breast Cancer: State of the Art

Plantamura

Cataldo

Cosentino

et al. 2020

IJMS

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Despite its controversial roles in different cancer types, miR-205 has been mainly described as an oncosuppressive microRNA (miRNA), with some contrasting results, in breast cancer. The role of miR-205 in the occurrence or progression of breast cancer has been extensively studied since the first evidence of its aberrant expression in tumor tissues versus normal counterparts. To date, it is known that the expression of miR-205 in the different subtypes of breast cancer is decreasing from the less aggressive subtype, estrogen receptor/progesterone receptor positive breast cancer, to the more aggressive, triple negative breast cancer, influencing metastasis capability, response to therapy and patient survival. In this review, we summarize the most important discoveries that have highlighted the functional role of this miRNA in breast cancer initiation and progression, in stemness maintenance, in the tumor microenvironment, its potential role as a biomarker and its relevance in normal breast physiology—the still open questions. Finally, emerging evidence reveals the role of some lncRNAs in breast cancer progression as sponges of miR-205. Here, we also reviewed the studies in this field.

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NFIB functions as an oncogene in estrogen receptor-positive breast cancer and is regulated by miR-205-5p

Cited by 8 publications

References 35 publications

Circ_0069718 promotes the progression of breast cancer by up-regulating NFIB through sequestering miR-590-5p

Circ_0069718 promotes the progression of breast cancer by up-regulating NFIB through sequestering miR-590-5p

Tumor-exosomal miR-205-5p as a diagnostic biomarker for colorectal cancer

miR-205 in Breast Cancer: State of the Art

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