NgR1 Expressed in P19 Embryonal Carcinoma Cells Differentiated by Retinoic Acid Can Activate STAT3

Lee, Su-In; Yun, Jieun; Baek, Joon Sang; Jeong, Yun-Ji; Kim, Jinah; Kang, Jong Soon; Park, Sun Hong; Kim, Sang Kyum; Park, Song‐Kyu

doi:10.4196/kjpp.2015.19.2.105

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…Nogo-66 influences embryonic stem cell self-renewal and differentiation via Stat3 signaling pathway [56] . Nogo-P4, an active segment of Nogo-66, is able to stimulate the phosphorylation of STAT3 and induce the expression of inducible nitricoxide synthase and cyclooxygenase-2 and the release of proinflammatory cytokines [57] , [58] . The Nogo-induced activation of NgR on microglia promotes the expression of proinflammatory cytokines and inhibits cell adhesion and migration behaviors [59] .…”

Section: Discussionmentioning

confidence: 99%

Knockout of the Nogo-B Gene Attenuates Tumor Growth and Metastasis in Hepatocellular Carcinoma

Zhu

Chen

et al. 2017

Neoplasia

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Human hepatocellular carcinoma (HCC) is a malignant cancer. It is a challenge to develop anti-HCC drugs due to HCC's extreme aggressiveness and with the sensitivity of the liver to show severe adverse effects. More importantly, the precise mechanisms causing HCC pathogenicity are not known. Our previous study disclosed Nogo-B as a reticulon 4 (Rtn4) family member. In the present study, we first identified that Nogo-B played a critical role in HCC progression. We found, via in vitro and in vivo assays, that Nogo-B was expressed aberrantly in primary HCC tumor tissues and immortal HCC cells but was relatively scarce in the normal liver tissues or cells. Nogo-B knockout, via the CRISPR-Cas9 technique, resulted in significant suppression of HCC cell proliferation and tumor growth. Next-generation sequencing analysis showed that Nogo-B knockout have effects on interleukin-6 (IL-6) signaling pathway. Furthermore, we observed that IL-6 induced phosphorylation of STAT3 (pSTAT3) in wild-type HCC cells, but Nogo-B knockout could reduce IL-6–induced increase of pSTAT3, supporting that Nogo-B affects HCC tumor progression possibly via regulating the IL-6/STAT3 signaling pathway. In conclusion, Nogo-B is expressed aberrantly in HCCs and plays an oncogenic role. These findings support that Nogo-B may be a novel anti-HCC therapeutic target.

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Section: Discussionmentioning

confidence: 99%

Knockout of the Nogo-B Gene Attenuates Tumor Growth and Metastasis in Hepatocellular Carcinoma

Zhu

Chen

et al. 2017

Neoplasia

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“…Excitability of the cells should also be considered, depending on the studied endpoint: even if they show excitability to some neuroactive molecules such as ACh, undifferentiated SH-SY5Y cells lack excitability to other ones such as glutamate and NMDA [14,15]. This lack of excitability may be attenuated in differentiated SH-SY5Y cells regarding their electrophysiological properties [41] and expression of neurotransmitter receptors such as glutamate [35,227]. Moreover, the central nervous system is insulated from the bloodstream by the blood-brain barrier that limits the passage of compounds such as neurotoxicants between both blood and neural tissues.…”

Section: Discussionmentioning

confidence: 99%

The SH-SY5Y human neuroblastoma cell line, a relevant in vitro cell model for investigating neurotoxicology in human: Focus on organic pollutants

et al. 2022

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“…The pluripotent mouse embryonal carcinoma cell line P19 can be induced to differentiate into neurons in the presence of all-trans-retinoid acid (RA). 1 P19 cells are widely used as an in vitro model for research on neuronal differentiation and its underlying molecular mechanism. 2 RA is a metabolite of retinol and plays an important role in the development of the central nervous system.…”

Section: Introductionmentioning

confidence: 99%

All‐trans‐retinoid acid induces the differentiation of P19 cells into neurons involved in the PI3K/Akt/GSK3β signaling pathway

et al. 2020

J of Cellular Biochemistry

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The pluripotent mouse embryonal carcinoma cell line P19 is widely used as a model for research on all‐trans‐retinoid acid (RA)‐induced neuronal differentiation; however, the signaling pathways involved in this process remain unclear. This study aimed to reveal the molecular mechanism underlying the RA‐induced neuronal differentiation of P19 cells. Real‐time quantitative polymerase chain reaction and Western blot analysis were used to determine the expression of neuronal‐specific markers, whereas flow cytometry was used to analyze cell cycle and cell apoptosis. The expression profiles of messenger RNAs (mRNAs) in RA‐induced neuronal differentiation of P19 cells were analyzed using high‐throughput sequencing, and the functions of differentially expressed mRNAs (DEMs) were determined by bioinformatics analysis. RA induced an increase in both class III β‐tubulin (TUBB3) and neurofilament medium (NEFM) mRNA expression, indicating that RA successfully induces neuronal differentiation of P19 cells. Cell apoptosis was not affected; however, cell proliferation decreased. We found 4117 DEMs, which were enriched in the phosphoinositide 3‐kinase/protein kinase B (PI3K/Akt) signaling pathway, Wnt signaling pathway, and cell cycle. Particularly, a few DEMs could be identified in the PI3K/Akt signaling pathway networks, such as PI3K, Akt, glycogen synthase kinase‐3β (GSK3β), cyclin‐dependent kinase 4 (CDK4), P21, and Bax. RA significantly increased the protein expression of PI3K, Akt, phosphorylated Akt, GSK3β, phosphorylated GSK3β, CDK4, and P21, but it reduced Bax protein expression. The Akt inhibitor affected the increase of TUBB3 and NEFM mRNA expression in RA‐induced P19 cells. The molecular mechanism underlying the RA‐induced neuronal differentiation of P19 cells is potentially involved in the PI3K/Akt/GSK3β signaling pathway. The decreased cell proliferation ability of neuronally differentiated P19 cells could be associated with the expression of cell cycle proteins.

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NgR1 Expressed in P19 Embryonal Carcinoma Cells Differentiated by Retinoic Acid Can Activate STAT3

Cited by 4 publications

References 28 publications

Knockout of the Nogo-B Gene Attenuates Tumor Growth and Metastasis in Hepatocellular Carcinoma

Knockout of the Nogo-B Gene Attenuates Tumor Growth and Metastasis in Hepatocellular Carcinoma

The SH-SY5Y human neuroblastoma cell line, a relevant in vitro cell model for investigating neurotoxicology in human: Focus on organic pollutants

All‐trans‐retinoid acid induces the differentiation of P19 cells into neurons involved in the PI3K/Akt/GSK3β signaling pathway

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