NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis

Chen, Zhi; Liang, Yusheng; Zong, Weicheng; Guo, Jiahe; Zhou, Jingpeng; Mao, Yongjiang; Ji, Dejun; Jiao, Peixin; J, LOOR Juan; Yang, Zhangping

doi:10.1016/s2095-3119(21)63812-1

Cited by 5 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The metabolism of glutamine to glutamate leads to the production of NH 4 + . Previous studies have shown that NH 4 + promotes apoptosis and inflammation [ 53 , 54 ], and this study provides the first evidence that NH 4 + induces MMPs in NP cells via the MAPK pathway. However, glutamine can be metabolized into α-ketoglutarate and enter the TCA cycle within the cell.…”

Section: Discussionsupporting

confidence: 61%

D-mannose alleviates intervertebral disc degeneration through glutamine metabolism

Dong,

Jiao,

Wang

et al. 2024

Military Med Res

View full text Add to dashboard Cite

Background Intervertebral disc degeneration (IVDD) is a multifaceted condition characterized by heterogeneity, wherein the balance between catabolism and anabolism in the extracellular matrix of nucleus pulposus (NP) cells plays a central role. Presently, the available treatments primarily focus on relieving symptoms associated with IVDD without offering an effective cure targeting its underlying pathophysiological processes. D-mannose (referred to as mannose) has demonstrated anti-catabolic properties in various diseases. Nevertheless, its therapeutic potential in IVDD has yet to be explored. Methods The study began with optimizing the mannose concentration for restoring NP cells. Transcriptomic analyses were employed to identify the mediators influenced by mannose, with the thioredoxin-interacting protein (Txnip) gene showing the most significant differences. Subsequently, small interfering RNA (siRNA) technology was used to demonstrate that Txnip is the key gene through which mannose exerts its effects. Techniques such as colocalization analysis, molecular docking, and overexpression assays further confirmed the direct regulatory relationship between mannose and TXNIP. To elucidate the mechanism of action of mannose, metabolomics techniques were employed to pinpoint glutamine as a core metabolite affected by mannose. Next, various methods, including integrated omics data and the Gene Expression Omnibus (GEO) database, were used to validate the one-way pathway through which TXNIP regulates glutamine. Finally, the therapeutic effect of mannose on IVDD was validated, elucidating the mechanistic role of TXNIP in glutamine metabolism in both intradiscal and orally treated rats. Results In both in vivo and in vitro experiments, it was discovered that mannose has potent efficacy in alleviating IVDD by inhibiting catabolism. From a mechanistic standpoint, it was shown that mannose exerts its anti-catabolic effects by directly targeting the transcription factor max-like protein X-interacting protein (MondoA), resulting in the upregulation of TXNIP. This upregulation, in turn, inhibits glutamine metabolism, ultimately accomplishing its anti-catabolic effects by suppressing the mitogen-activated protein kinase (MAPK) pathway. More importantly, in vivo experiments have further demonstrated that compared with intradiscal injections, oral administration of mannose at safe concentrations can achieve effective therapeutic outcomes. Conclusions In summary, through integrated multiomics analysis, including both in vivo and in vitro experiments, this study demonstrated that mannose primarily exerts its anti-catabolic effects on IVDD through the TXNIP-glutamine axis. These findings provide strong evidence supporting the potential of the use of mannose in clinical applications for alleviating IVDD. Compared to existing clinically invasive or pain-relieving therapies for IVDD, the oral administration of mannose has characteristics that are more advantageous for clinical IVDD treatment.

show abstract

Section: Discussionsupporting

confidence: 61%

D-mannose alleviates intervertebral disc degeneration through glutamine metabolism

Dong,

Jiao,

Wang

et al. 2024

Military Med Res

View full text Add to dashboard Cite

show abstract

“…Compared with the control, overexpression of circRNA-08409 significantly downregulated miR-133a and upregulated mRNA TGFB2 while inducing apoptosis. Subsequently, a NH 4 Cl-induced mastitis model was established, and the role of circRNA in regulating apoptosis and inflammation in bovine mammary epithelial cells was verified via the circ02771/miR-194b/TGIF1 axis ( Chen et al, 2022 ). Both of the abovementioned studies not only illustrate that circRNAs can act as an indirect regulator in bovine mastitis by targeting complementary miRNAs, but also highlight that environmental chemicals could be potential factors for mammary gland inflammation in dairy bred management.…”

Section: Circular Rnasmentioning

confidence: 99%

“…Bovine mastitis, an inflammatory disease of the mammary gland, is recognized as one of the most troublesome and costly problems in modern dairy industry. There are several factors contributing to mastitis that is notorious for its high morbidity, including pathogenetic microbial infections ( Nash et al, 2003 ), physical trauma and environmental hazards ( Chen et al, 2021 , 2022 ). Hitherto over 130 pathogens are demonstrated to be related to mastitis ( Bradley, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Emerging roles of noncoding micro RNAs and circular RNAs in bovine mastitis: Regulation, breeding, diagnosis, and therapy

et al. 2022

Self Cite

View full text Add to dashboard Cite

Bovine mastitis is one of the most troublesome and costly problems in the modern dairy industry, which is not only difficult to monitor, but can also cause economic losses while having significant implications on public health. However, efficacious preventative methods and therapy are still lacking. Moreover, new drugs and therapeutic targets are in increasing demand due to antibiotic restrictions. In recent years, noncoding RNAs have gained popularity as a topic in pathological and genetic studies. Meanwhile, there is growing evidence that they play a role in regulating various biological processes and developing novel treatment platforms. In light of this, this review focuses on two types of noncoding RNAs, micro RNAs and circular RNAs, and summarizes their characterizations, relationships, potential applications as selection markers, diagnostic or treatment targets and potential applications in RNA-based therapy, in order to shed new light on further research.

show abstract

“…The 4D properties in ABS-based composite and industrial uses of NH4Cl and ZnCl2 electrolytes have been reported for energy harvesting in nano-power generator devices and ESD. This indicates that such materials may also be used as sensors to monitor the health of ESD prepared by 3D printing processes [19][20][21][22][23]. Some studies outlined that the functional prototypes of ABS and its electro-active composites fabricated by single or multi-material 3D printing processes outlined good mechanical, structural, thermal, and electrical properties in ABS for one-way programmable sensing applications [24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

3D printed sensor for online condition monitoring of energy storage device

et al. 2022

View full text Add to dashboard Cite

In the past two decades' significant studies have been reported on electrically conducting ther-moplastic composites of acrylonitrile butadiene styrene (ABS), polyvinylidene fluoride (PVDF), etc. for the fabrication of novel energy storage devices (ESD) by 3D printing. But hitherto little has been reported on online condition monitoring of ESD prepared by secondary (2°) recycling of ABS. This study reports the investigations on mechanical and electrical properties of NH4Cl-ZnCl2 (electrolyte) reinforced ABS composite (as 3D printed sensor) for online condition monitoring of ESD. In a typical dry cell, the electrolyte is one of the integral parts, and the change in its dielectric properties with the time/ applied electric load has been used to ascertain the health of ESD (online) as the internet of things (IoT) based solution (Bluetooth application) in industry sports and medicine (ISM) band (2.4 GHz). Based on melt flow index (MFI), 10% NH4Cl and 10% ZnCl2 (by weight%) were reinforced in ABS for preparing 3D printed rectangular substrates as ring resonators for cal-culating dielectric constant (er) and loss tangent/dissipation factor (tand) for the resonant frequency. Trans-mission line parameters (S21) were observed using a vector network analyzer (VNA), and a high-frequency structure simulation (HFSS) software package. The results are supported by morphological analysis of ABS composite based on scanning electron microscopy (SEM), energy dispersion spectroscopy (EDS), 3D rendering, surface roughness (Ra), area mapping, current (I)-voltage (V), and Fourier transformed infrared (FTIR) characterization.

show abstract

NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis

Cited by 5 publications

References 38 publications

D-mannose alleviates intervertebral disc degeneration through glutamine metabolism

D-mannose alleviates intervertebral disc degeneration through glutamine metabolism

Emerging roles of noncoding micro RNAs and circular RNAs in bovine mastitis: Regulation, breeding, diagnosis, and therapy

3D printed sensor for online condition monitoring of energy storage device

Contact Info

Product

Resources

About