Nichtenzymatische TNBT-F�rbung von Gewebsstrukturen

Wohlrab, F; Fuchs, U

doi:10.1007/bf00294393

Cited by 14 publications

(1 citation statement)

References 33 publications

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“…Melnick [8] when comparing a number of fixatives, found that pure cold acetone yields best results in a compromise between enzyme in activation and maintenance of cell morphology. Apart from the mainte nance of cellular appearance, the acetone fixation is advantageous, since the extraction of lipoids from the cells reduces formazan precipitation at enzyme free locations through absoption at lipid containing structures [4,6,9,15,16].…”

Section: Discussionmentioning

confidence: 99%

Enzyme Cytochemistry of the Energy Metabolism in the Cells of Normal Human Bone Marrow

Zurwehme¹,

Mitzkat²,

Dommes³

1971

Acta Haematol

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Using a gel film technique 11 key enzymes of the energy metabolism were histochemically demonstrated in the bone marrow cells of healthy persons or patients suffering from non-haematological disorders. The enzyme activity was assessed by a semiquantitative scoring method. A reduction of all enzymatic activities could be seen in the erythropoiesis during maturation. No histochemically visible change of enzymatic activity was observed with increasing differentiation of the granulopoiesis in the LDH, GAPDH, MDH, ME, GLUDH, GDH and HK. However, the enzymatic activity increased in the PGLUM and G6PDH whereas the SDH- and GPOX-activity diminished with the maturation of the granulopoiesis. It was concluded that the gel film technique may reveal increasing or diminishing alterations in the enzymatic pattern in diseases of the bone marrow.

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