Nickel Nanoparticle Modified BDD Electrode Shows an Electrocatalytic Response to Adenine and DNA in Aqueous Alkaline Media

Harfield, John C.; Toghill, Kathryn E.; Batchelor‐McAuley, Christopher; Downing, Clive; Compton, Richard G.

doi:10.1002/elan.201000658

Cited by 36 publications

(25 citation statements)

References 37 publications

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“…Sensing the repair activity of this damage is complicated by the fact that repair enzymes that fix 8-oxoguanine base lesions bind to DNA nonspecifically, such that enzymatic binding and excision activity are decoupled. 8-Oxoguanine itself has been distinguished by GC-MS and HPLC-coupled electrochemical detection (Abalea et al 1999; Ivandini et al 2007; Ravanat et al 1998; Rebelo et al 2004), and electrochemical devices were used to indirectly study DNA damage (Cahova-Kucharikova et al 2005; Harfield et al 2011; Havran et al 2008; Oliveira-Brett et al 2002; Wang et al 1997). However, these techniques and devices have not offered a real-time quantitative measure of repair of oxidative DNA damage.…”

Section: Introductionmentioning

confidence: 99%

Using DNA devices to track anticancer drug activity

Kahanda

Chakrabarti

McWilliams

et al. 2016

Biosensors and Bioelectronics

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It is beneficial to develop systems that reproduce complex reactions of biological systems while maintaining control over specific factors involved in such processes. We demonstrated a DNA device for following the repair of DNA damage produced by a redox-cycling anticancer drug, beta-lapachone (β-lap). These chips supported ß-lap-induced biological redox cycle and tracked subsequent DNA damage repair activity with redox-modified DNA monolayers on gold. We observed drug-specific changes in square wave voltammetry from these chips at therapeutic ß-lap concentrations of high statistical significance over drug-free control. We also demonstrated a high correlation of this change with the specific ß-lap-induced redox cycle using rational controls. The concentration dependence of ß-lap revealed significant signal changes at levels of high clinical significance as well as sensitivity to sub-lethal levels of ß-lap. Catalase, an enzyme decomposing peroxide, was found to suppress DNA damage at a NQO1/catalase ratio found in healthy cells, but was clearly overcome at a higher NQO1/catalase ratio consistent with cancer cells. We found that it was necessary to reproduce key features of the cellular environment to observe this activity. Thus, this chip-based platform enabled tracking of ß-lap-induced DNA damage repair when biological criteria were met, providing a unique synthetic platform for uncovering activity normally confined to inside cells.

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Section: Introductionmentioning

confidence: 99%

Using DNA devices to track anticancer drug activity

Kahanda

Chakrabarti

McWilliams

et al. 2016

Biosensors and Bioelectronics

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“…[33] The catalytic features of Ni electrodes are directly related to the electrochemistry of Ni(OH) 2 film layers that form spontaneously on the Ni surface. [34] Ni electrodes, or in situ generated Ni-nanoparticle-modified electrodes, have been previously used for the analysis of methanol, [35] sulfide, [36] and adenine [37] by exploiting the catalytic features of the Ni(OH) 2 /NiOOH redox couple. It is surprising that such catalytic effects have not been explored in correlation with the Ni-based metallic impurities present in CNTs.…”

Section: Introductionmentioning

confidence: 99%

Redox‐Active Nickel in Carbon Nanotubes and Its Direct Determination

Ambrosi

Pumera

2012

Chemistry A European J

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The presence of residual metal-catalyst impurities in carbon nanotubes is responsible for their toxicity. It is important to differentiate between the total amount of impurities and the redox-active (bioavailable) amount of such impurities because only the bioavailable impurities exhibit toxic effects. Herein, we report a simple and specific method for quantifying the amount of redox-active Ni present in various commercial samples of CNTs. It is based on the electrochemical oxidation of Ni(OH)(2) that is formed in alkaline solutions when Ni impurities are opened to the surrounding environment. Metallic Ni impurities play an extremely active role in toxicological assays as well as in undesired catalytic processes, and thus a method to rapidly quantify the amount of redox-active Ni is of great importance.

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“…Eithne et al have testified both Ni/C and Co-Ni/C electrodes have catalytic properties towards uric acid [21]. Richard and his group members have verified the nickel nanoparticle modified boron-doped diamond electrodes' electrooxidation property of G and A in aqueous alkaline media [22]. Nickel or nickel based nanoparticles revealed extraordinary electrocatalyitc properties in the direct oxidation of small molecules and exhibited a good compatibility when embedding them with carbon based materials.…”

Section: G] = [C] and [A] = [T]mentioning

confidence: 97%

“…It has been reported that the electrochemical oxidation of G and A both followed a two-step mechanism and the first 2e À oxidation process was rate determining step [35][36][37][38]. Scheme 1 is the mechanism of electrochemical oxidation process of G [22].…”

Section: The Optimization Of Buffer Phmentioning

confidence: 99%