Nickel oxide (NiO) nanoparticles disturb physiology and induce cell death in the yeast Saccharomyces cerevisiae

Sousa, Cátia A.; Soares, Helena; Soares, Eduardo V.

doi:10.1007/s00253-018-8802-2

Cited by 19 publications

(32 citation statements)

References 47 publications

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“…cerevisiae (Sousa, Soares, & Soares, 2018). The ability of NiO-NPs to induce apoptosis, DNA damage, oxidative stress and alterations in the transcriptome of human liver cells (HepG2) has been well documented (Saquib et al, 2018).…”

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confidence: 99%

Retracted: In vitro genotoxicity assessment of nickel(II) oxide nanoparticles on lymphocytes of human peripheral blood

Dumala

Mangalampalli

Grover

2019

J of Applied Toxicology

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The current study was intended to elucidate the cytotoxicity, genotoxicity ability of nickel oxide (NiO) nanoparticles (NPs) and assessment of preliminary mechanism of the toxicity. Characterization studies showed that NiO‐NPs have a particle size of 17.94 (±3.48) nm. The particle size of the NPs obtained by dynamic light scattering method in Milli‐Q and RPMI 1640 media was 189.9 (±17.1) and 285.9 (±19.6) nm, respectively. The IC50 concentration for NiO‐NPs after 24 hours of treatment was estimated as 23.58 μg/mL. Comet and cytokinesis‐block micronucleus assays revealed a significant dose‐ and time‐dependent genotoxic potential of NiO‐NPs. Morphological assessment of the lymphocytes upon exposure to NiO‐NPs showed that the mechanism of toxicity was apoptosis. Reactive oxygen species analysis and lipid peroxidation patterns were aligned with the cytotoxicity and genotoxicity endpoints. Thus, the preliminary mechanism of NiO‐NPs for cytotoxicity on lymphocytes was assumed to be oxidative stress‐mediated apoptosis and DNA damage. Furthermore, these NiO‐NPs are considered a potentially hazardous substance at environmentally significant levels. Further investigations are suggested to understand the immunotoxic effects of NiO‐NPs.

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confidence: 99%

Retracted: In vitro genotoxicity assessment of nickel(II) oxide nanoparticles on lymphocytes of human peripheral blood

Dumala

Mangalampalli

Grover

2019

J of Applied Toxicology

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“…NiO NPs are used in solar cells, lithium-ion batteries, diodes and biosensors (Ates, Demir, Arslan, Camas, & Celik, 2016). Furthermore, NiO NPs have been the material of interest for their diesel-fuel additive and pigment properties (Sousa, Soares, & Soares, 2018). Although Ni and its compounds were classified as class 1 carcinogens (IARC, 1990), increase in the use of NiO NPs makes it necessary to have a clear understanding of their probable effect on the health of humans and the environment.…”

Section: Introductionmentioning

confidence: 99%

Repeated oral dose toxicity study of nickel oxide nanoparticles in Wistar rats: a histological and biochemical perspective

Dumala

Mangalampalli

Kamal

et al. 2019

J of Applied Toxicology

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Despite the increasing use of nickel oxide (NiO) nanoparticles (NPs), limited information is available on their toxicological effects. Health consequences of 28 days repeated oral exposure to NiO NPs have not been explored thoroughly. Hence, toxicity investigations were performed after 28‐day daily exposure in albino Wistar rats with NiO NPs following Organization for Economic Co‐operation and Development test guideline 407. Histopathology, biochemical indices including oxidative stress and biodistribution patterns were evaluated to decipher the toxicological impact of NiO NPs. NiO NP characterization by transmission electron microscopy showed an average size of 12.9 (±3.4) nm. Histological studies depicted a prominent impact on the vital organs of the rats. A dose‐dependent rise in both aminotransferase enzyme values was recorded in the homogenates of liver and kidney tissues. A significant decrease in superoxide dismutase activity and increase in catalase activity was noted. Further, a dose‐dependent decrease in reduced glutathione content was recorded in rats, which suggested generation of reactive oxygen species and oxidative stress. Increase in the malondialdehyde levels was observed with an increase in the dose substantiating the antioxidant enzyme activity profiles. Biodistribution studies indicated maximum accumulation of Ni content in liver followed by kidney. Excretion of Ni was predominantly through feces and a little through renal clearance. Our study indicated that NiO NPs adversely alter the biochemical profile of the rats and cause histological damage. Further investigations are warranted to address the mechanism by which physiological path these NiO NPs exhibit their toxic nature in in vivo.

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“…The main characteristics of Al 2 O 3 , In 2 O 3 , Mn 3 O 4 , SiO 2 and SnO 2 NPs used in the present work (namely, purity, particle size and surface area) can be found in Supplementary Table S.1. The purity of NPs was determined by their digestion with aqua regia, as previously described (Sousa et al 2018); metal (Al, In, Mn and Sn) content was determined by atomic absorption spectroscopy with flame atomization (AAS-FA) in an Analytik Jena novAA 350 spectrometer (Analytik Jena; Jena, Germany).…”

Section: Nps Characteristics and Stock Suspension Preparationmentioning

confidence: 99%

“…NPs stock suspensions at 0.5 g/L were prepared in deionized water. The suspensions were vigorously shaken, sonicated for 15 min in an ultrasonic bath (80-160 W; Bandelin, Sonorex RK 100; Berlin, Germany) and sterilized by UV light, as previously described (Sousa et al 2018). NPs stock suspensions were stored in the dark for up to 1 month, at 4°C.…”

Section: Nps Characteristics and Stock Suspension Preparationmentioning

confidence: 99%

“…MOx NPs were suspended at a final concentration of 100 mg/L and incubated in the same conditions of the assays with yeast cells, as described below. The Z-average diameter and the zeta potential were measured using a Zetasizer Nano ZS (Malvern Instruments; Malvern, UK), coupled with Zetasizer Software version 7.11, as previously described (Sousa et al 2018).…”

Section: Characterization Of Nps Suspensions In Different Mediamentioning

confidence: 99%

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Metal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen species

Sousa

Soares

2019

Appl Microbiol Biotechnol

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In this work, the physicochemical characterization of five (Al 2 O 3 , In 2 O 3 , Mn 3 O 4 , SiO 2 and SnO 2) nanoparticles (NPs) was carried out. In addition, the evaluation of the possible toxic impacts of these NPs and the respective modes of action were performed using the yeast Saccharomyces cerevisiae. In general, in aqueous suspension, metal(loid) oxide (MOx) NPs displayed an overall negative charge and agglomerated; these NPs were practically insoluble (dissolution < 8%) and did not generate detectable amounts of reactive oxygen species (ROS) under abiotic conditions. Except In 2 O 3 NPs, which did not induce an obvious toxic effect on yeast cells (up to 100 mg/L), the other NPs induced a loss of cell viability in a dose-dependent manner. The comparative analysis of the loss of cell viability induced by the NPs with the ions released by NPs (NPs supernatant) suggested that SiO 2 toxicity was mainly caused by the NPs themselves, Al 2 O 3 and SnO 2 toxic effects could be attributed to both the NPs and the respective released ions and Mn 3 O 4 harmfulness could be mainly due to the released ions. Al 2 O 3 , Mn 3 O 4 , SiO 2 and SnO 2 NPs induced the loss of metabolic activity and the generation of intracellular ROS without permeabilization of plasma membrane. The co-incubation of yeast cells with MOx NPs and a free radical scavenger (ascorbic acid) quenched intracellular ROS and significantly restored cell viability and metabolic activity. These results evidenced that the intracellular generation of ROS constituted the main cause of the cytotoxicity exhibited by yeasts treated with the MOx NPs. This study highlights the importance of a ROS-mediated mechanism in the toxicity induced by MOx NPs.

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Nickel oxide (NiO) nanoparticles disturb physiology and induce cell death in the yeast Saccharomyces cerevisiae

Cited by 19 publications

References 47 publications

Retracted: In vitro genotoxicity assessment of nickel(II) oxide nanoparticles on lymphocytes of human peripheral blood

Retracted: In vitro genotoxicity assessment of nickel(II) oxide nanoparticles on lymphocytes of human peripheral blood

Repeated oral dose toxicity study of nickel oxide nanoparticles in Wistar rats: a histological and biochemical perspective

Metal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen species

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