Nicking enzyme-free strand displacement ampliﬁcation-assisted CRISPR-Cas-based colorimetric detection of prostate-specific antigen in serum samples

Wang, Wanhe; Liu, Jianhua; Wu, Lian; Ko, Chung-Nga; Wang, Xueliang; Lin, Chuankai; Jing-qi, Liu; Ling, Liansheng; Wang, Jing

doi:10.1016/j.aca.2022.339479

Cited by 19 publications

(8 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also has good compatibility with various signal probes. These attributes render SDA an innovative signal amplification methodology with substantial potential for numerous types of biosensors, encompassing colorimetry, fluorescence, and chemiluminescence ( Wang et al, 2022 ).…”

Section: Pathogen Detection Based On Isothermal Nucleic Acid Amplific...mentioning

confidence: 99%

Combination of nucleic acid amplification and CRISPR/Cas technology in pathogen detection

Zeng,

Jiao,

2024

Front. Microbiol.

View full text Add to dashboard Cite

Major health events caused by pathogenic microorganisms are increasing, seriously jeopardizing human lives. Currently PCR and ITA are widely used for rapid testing in food, medicine, industry and agriculture. However, due to the non-specificity of the amplification process, researchers have proposed the combination of nucleic acid amplification technology with the novel technology CRISPR for detection, which improves the specificity and credibility of results. This paper summarizes the research progress of nucleic acid amplification technology in conjunction with CRISPR/Cas technology for the detection of pathogens, which provides a reference and theoretical basis for the subsequent application of nucleic acid amplification technology in the field of pathogen detection.

show abstract

Section: Pathogen Detection Based On Isothermal Nucleic Acid Amplific...mentioning

confidence: 99%

Combination of nucleic acid amplification and CRISPR/Cas technology in pathogen detection

Zeng,

Jiao,

2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…For example, AuNPs coupled with Cas12a collateral digestion and RCA amplification were used for colorimetric CRISPR-Cas sensing, where aptamer/crRNA/Cas12a ternary complexes cleave primer sequences and padlock probes modified on AuNPs. Wang et al [ 69 ] used distance-dependent optical properties of AuNPs and nicking enzyme-free amplification to produce more acDNA and detected aflatoxin M1 (AFM1) with ppb level of accuracy and sensing of serum PSA [ 69 , 70 ].…”

Section: Colorimetry-based Sensingmentioning

confidence: 99%

Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

et al. 2023

View full text Add to dashboard Cite

CRISPR-Cas systems have been widely used in genome editing and transcriptional regulation. Recently, CRISPR-Cas effectors are adopted for biosensor construction due to its adjustable properties, such as simplicity of design, easy operation, collateral cleavage activity, and high biocompatibility. Aptamers’ excellent sensitivity, specificity, in vitro synthesis, base-pairing, labeling, modification, and programmability has made them an attractive molecular recognition element for inclusion in CRISPR-Cas systems. Here, we review current advances in aptamer-based CRISPR-Cas sensors. We briefly discuss aptamers and the knowledge of Cas effector proteins, crRNA, reporter probes, analytes, and applications of target-specific aptamers. Next, we provide fabrication strategies, molecular binding, and detection using fluorescence, electrochemical, colorimetric, nanomaterials, Rayleigh, and Raman scattering. The application of CRISPR-Cas systems in aptamer-based sensing of a wide range of biomarkers (disease and pathogens) and toxic contaminants is growing. This review provides an update and offers novel insights into developing CRISPR-Cas-based sensors using ssDNA aptamers with high efficiency and specificity for point-of-care setting diagnostics.

show abstract

“…A prostate-specific antigen was used in Wang's experiment to design a unique sequence that would be vulnerable to CRISPR-Cas12a cleavage. This method produces a product that can be quantified via the colorimetric method and used to diagnose prostate cancer [118].…”

Section: Enzyme-dependent Biosensingmentioning

confidence: 99%

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Chan

Chang

et al. 2023

Molecules

View full text Add to dashboard Cite

Molecular biology applications based on gold nanotechnology have revolutionary impacts, especially in diagnosing and treating molecular and cellular levels. The combination of plasmonic resonance, biochemistry, and optoelectronic engineering has increased the detection of molecules and the possibility of atoms. These advantages have brought medical research to the cellular level for application potential. Many research groups are working towards this. The superior analytical properties of gold nanoparticles can not only be used as an effective drug screening instrument for gene sequencing in new drug development but also as an essential tool for detecting physiological functions, such as blood glucose, antigen-antibody analysis, etc. The review introduces the principles of biomedical sensing systems, the principles of nanomaterial analysis applied to biomedicine at home and abroad, and the chemical surface modification of various gold nanoparticles.

show abstract

Nicking enzyme-free strand displacement ampliﬁcation-assisted CRISPR-Cas-based colorimetric detection of prostate-specific antigen in serum samples

Cited by 19 publications

References 54 publications

Combination of nucleic acid amplification and CRISPR/Cas technology in pathogen detection

Combination of nucleic acid amplification and CRISPR/Cas technology in pathogen detection

Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Contact Info

Product

Resources

About