Nicotinamide mononucleotide supplementation protects the intestinal function in aging mice and <scp>d</scp>-galactose induced senescent cells

Ru, Meng; Wang, Wanwan; Zhai, Zhe; Wang, Ruxia; Li, Yumeng; Jiang, Liang; Kothari, Damini; Niu, Kai-Yang; Wu, Xin

doi:10.1039/d2fo00525e

Cited by 37 publications

(19 citation statements)

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“…The relative levels of gene expression were normalized with β-actin as a housekeeping gene and then calculated using the comparative CT method (2 −ΔΔCt ) as shown in our previous study. 20…”

Section: Methodsmentioning

confidence: 99%

Taurochenodeoxycholic acid inhibits intestinal epithelial cell proliferation and induces apoptosis independent of the farnesoid X receptor

et al. 2023

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Section: Methodsmentioning

confidence: 99%

Taurochenodeoxycholic acid inhibits intestinal epithelial cell proliferation and induces apoptosis independent of the farnesoid X receptor

et al. 2023

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“…We found that NMN supplementation restored the nuclear transfer of Nrf2 and effectively upregulated the expression of these antioxidant genes. Recent studies have also shown that NMN can upregulate the expression of Nrf2 and enhance the antioxidant ability of intestinal cells [ 39 ]. These results suggest that the protective effect of NMN against oxidative stress is regulated by Nrf2.…”

Section: Discussionmentioning

confidence: 99%

Nicotinamide Mononucleotide Ameliorates Silica-Induced Lung Injury through the Nrf2-Regulated Glutathione Metabolism Pathway in Mice

et al. 2022

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Nicotinamide mononucleotide (NMN) is a natural antioxidant approved as a nutritional supplement and food ingredient, but its protective role in silicosis characterized by oxidative damage remains unknown. In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis. We found that NMN mitigated lung damage at 7 and 28 days, manifested as a decreasing coefficient of lung weight and histological changes, and alleviated oxidative damage by reducing levels of reactive oxygen species and increasing glutathione. Meanwhile, NMN treatment also reduced the recruitment of inflammatory cells and inflammatory infiltration in lung tissue. Transcriptomic analysis showed that NMN treatment mainly regulated immune response and glutathione metabolism pathways. Additionally, NMN upregulated the expression of antioxidant genes Gstm1, Gstm2, and Mgst1 by promoting the expression and nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2). Gene interaction analysis showed that Nrf2 interacted with Gstm1 and Mgst1 through Gtsm2. Promisingly, oxidative damage mediated by these genes occurred mainly in fibroblasts. In summary, NMN alleviates silica-induced oxidative stress and lung injury by regulating the endogenous glutathione metabolism pathways. This study reveals that NMN supplementation might be a promising strategy for mitigating oxidative stress and inflammation in silicosis.

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“…The microbiota analyses mainly followed the methods described in previous studies. 18,19 The total genomic DNA (gDNA) of each colonic content sample was extracted using a QIAamp DNA isolation kit (Qiagen, Hilden, Germany). The concentration and the integrity of the isolated gDNA were assessed using a Nanodrop 2000 (TermoFisher Scientific, USA) and 1.5% agarose gel electrophoresis, respectively.…”

Section: Gut Microbiota Analysismentioning

confidence: 99%

Stepwise co‐fermented traditional Chinese medicine byproducts improve antioxidant and anti‐inflammatory effects in a piglet model

Niu,

Wang,

Kim

et al. 2023

J Sci Food Agric

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BackgroundLianhua‐Qingwen (LHQW) capsule is a Traditional Chinese Medicine (TCM) formula having anti‐viral and anti‐inflammatory activities. During the capsule production, huge byproducts will be yielded and disposed as waste by burying. Resourceful utilization of this kind of TCM byproducts as feed additives through stage‐based co‐fermentation using enzyme and probiotics could reduce environmental stress and resource shortage. The in vitro characterization and the supplementary effects of the fermented TCM byproducts (FTCM) for weaned piglets (initial body weight, 7.23 ± 0.33 kg; dose: basal diet + 300 mg/kg FTCM) were investigated.ResultsHigher reducing sugar content, total flavonoid content, flavonoid compounds (e.g. tectoridin, tricetin, flavone, apigenin, naringenin), and total antioxidant activity were determined in the FTCM compared to the spontaneously fermented and unfermented materials. Supplementation of the FTCM to piglets did not significantly affect the feed intake, body weight gain, and feed/gain ratio, but significantly decreased a pro‐inflammatory cytokine IL‐8 and increased intestinal total antioxidant activity (TAC) and superoxide dismutase (SOD) activity. Moreover, FTCM supplementation increased α‐diversity of the colonic microbiota accompanied with increased abundance of Prevotella genus and Treponema berlinense species. Correlation analysis indicates T. berlinense is responsible for the decreased IL‐8 level and enhanced intestinal TAC and SOD activities which might be mediated by a homoserine lactone molecule (3‐oxo‐C14).ConclusionOverall, the stepwise co‐fermentation enriched bioactive compounds within the TCM byproducts and its dietary supplementation did not generate any side effect on growth performance but displayed beneficial effects on enrichment of potential probiotic T. berlinense and relevant functions.This article is protected by copyright. All rights reserved.

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Nicotinamide mononucleotide supplementation protects the intestinal function in aging mice and d-galactose induced senescent cells

Abstract: Aging is concomitant with the decline of various physiological functions. Nicotinamide mononucleotide (NMN) is an important precursor of nicotinamide adenine dinucleotide (NAD+) that shows potential to retard aging. Herein, we...

Cited by 37 publications

References 50 publications

Taurochenodeoxycholic acid inhibits intestinal epithelial cell proliferation and induces apoptosis independent of the farnesoid X receptor

Taurochenodeoxycholic acid inhibits intestinal epithelial cell proliferation and induces apoptosis independent of the farnesoid X receptor

Nicotinamide Mononucleotide Ameliorates Silica-Induced Lung Injury through the Nrf2-Regulated Glutathione Metabolism Pathway in Mice

Stepwise co‐fermented traditional Chinese medicine byproducts improve antioxidant and anti‐inflammatory effects in a piglet model

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